Macrowine 2021
IVES 9 IVES Conference Series 9 IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

Abstract

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA). The analyses of polyphenols and IBMP in model wine were all performed in 10ml vial flasks with volumes of 1ml of model wine volume. IBMP was quantified in Single Ion Monitoring (SIM) mode. The ions used for SIM mode were m/z 94, 121, 154. All the solutions were at fixed pH and ethanol concentration (pH 3.5, 12% Ethanol). For each experiment a control of IBMP, polyphenol, a blank and a mix between IBMP and polyphenol were prepared. 2. Sensory Analysis: Triangular tests were performed by trained panelists (n=26) to orthonasally differentiate between IBMP on its own and IBMP in the presence of a polyphenol. 3. NMR Spectroscopy : Spectra were performed on an Agilent 500 MHz DD2 NMR spectrometer (Agilent Technologies, Santa Clara, CA, USA).Titrations of polyphenols were obtained by addition of IBMP solubilized in CD3OD (~200mM) to quercetin or polydatin (~20mM) CD3OD solution. Absolute concentrations of both aroma and polyphenols were accurately determined from the surface integration of well-separated 1D 1H signal using qNMR method. Results: 1. The headspace analysis showed that polydatin and quercetin decreased the volatility of IBMP. 2. Compared to the control (IBMP without polyphenol), significant results were found in sensory analysis (triangular tests) for polytadin (P < 0030) and quercetin (P < 0000) in presence of IBMP at 8 ng/L in model wine solution 3. Variations in NMR 1H chemical shifts were observed in titration experiments which suggest different possible sites for the interactions of IBMP with the polyphenols

[1] D. Sidhu, J.Lund, Y. Kotseridis, C.Saucier (2015). Methoxypyrazine Analysis and influence of Viticultural and Enological Procedures on their Levels in Grapes, Musts, and Wines, Critical reviews in Food Science and Nutrition, 55:4, 485-502, [2]. Aronson, J., & Ebeler, S. E. (2004). Effect of polyphenol compounds on the headspace volatility of flavors. American Journal of Enology and Viticulture, 55 :1, 13-21.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Cédric Saucier*, Audrey Bloem, Christine Le Guernevé, Damien Lorenzi, Elizabeth Johansson Filote, Nicolas Bouvier, Peggy Rigou, Veronique Cheynier

*Université de Montpellier

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Determination of metallic elements in Chilean wines by atomic absorption spectroscopy and inductively coupled plasma–mass spectrometry

The chemical composition of wines depends on series of variables such as the type of grape, edaphoclimatic conditions, and viticulture and winemaking practices employed during production. Metallic elements play a significant role during winemaking (e.g. as catalysts of oxidation reactions) and have been previously employed for the classification of wines according to provenance. In this work, we focused on the analysis of metallic elements (K, Na, Ca, Zn, Cu, Fe, Mg, Mn, Ni, Cr, Al, Pb, Cd, Hg, Se, Co, Sn and As) in 145 Chilean wine samples (102 reds and 43 white wines), of seven grape varieties, and five of the major wine producing regions in Chile.

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

Use of glutathione under different grape processing and winemaking conditions and its impact on the formation of sulfide off-flavors, colour, and sensory characteristics of Riesling, Sauvignon blanc, and Chardonnay

The use of glutathione (GSH) in winemaking has been legitimated recently, according to OIV resolutions OENO 445-2015 and OENO 446-2015 a maximum dose of 20 mg/L is now allowed to use in must and wine. Several studies have proven the benefits of GSH, predominantly in Sauvignon blanc. Thus, oxidative coloration of must and wine is limited, aroma compounds such as volatile thiols are preserved, and the development of ageing flavors such as sotolon and 2-aminoacetophenone is impeded. The protective effect may be explained by the high affinity of GSH to bind o-quinones which are formed during phenolic oxidation and which are known to initiate browning and other oxidative changes. Some researchers have proposed the hydroxycinnamic acid to GSH ratio (HGR) as an indicator of oxidation susceptibility of must and could show that lower ratios yielded lighter musts.

The impact of different yeasts and harvest time on the wine quality of Beihong and Beimei (<I>V. vinifera x V. amurensis</I>)

Beihong and Beimei are two wine cultivars from ‘Muscat Hamberg’ (V. vinifera L.) and wild V. amurensis Rupr., which were released in China in 2008. Here,two enology practices were reported. Firstly, the impact of different yeasts including D254, GRE, K1, D21 and BDX on dry wine quality of Beihong and Beimei was investigated. For Beihong, among wines fermented by all yeasts, residual sugar content was the lowest, total anthocyanin and resveratrol contents were the highest in the wine by D254. However, the wine by D254 had lower titrable acid than those by the other yeasts except BDX.

Influence of inactive dry yeast treatments during grape ripening on postharvest berry skin texture parameters and phenolic compounds extractability

Inactive dry yeast treatments in the vineyard are a tool used with the aim to improve the concentration and quality of secondary metabolites in grapes, leading to a better differentiation of the wines made from grapes differently treated. In this work, a foliar spraying treatment with yeast derivatives specifically designed to be used with the patent pending application technology of Lallemand Inc. Canada (LalVigne® Mature, Lallemand Inc., Montreal, Canada) was tested on Vitis vinifera L. cv. Barbera and Nebbiolo black winegrapes. The aim was to evaluate the effect of this treatment on the phenolic compounds accumulation, the skin physical-mechanical properties and the related phenolic extractability. Prior to analysis, the berries were sorted by flotation in order to evaluate their distribution by density class, and to determine the skin texture parameters of berries with different sugar contents, thus understanding also the ripening effect.