Macrowine 2021
IVES 9 IVES Conference Series 9 IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

Abstract

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA). The analyses of polyphenols and IBMP in model wine were all performed in 10ml vial flasks with volumes of 1ml of model wine volume. IBMP was quantified in Single Ion Monitoring (SIM) mode. The ions used for SIM mode were m/z 94, 121, 154. All the solutions were at fixed pH and ethanol concentration (pH 3.5, 12% Ethanol). For each experiment a control of IBMP, polyphenol, a blank and a mix between IBMP and polyphenol were prepared. 2. Sensory Analysis: Triangular tests were performed by trained panelists (n=26) to orthonasally differentiate between IBMP on its own and IBMP in the presence of a polyphenol. 3. NMR Spectroscopy : Spectra were performed on an Agilent 500 MHz DD2 NMR spectrometer (Agilent Technologies, Santa Clara, CA, USA).Titrations of polyphenols were obtained by addition of IBMP solubilized in CD3OD (~200mM) to quercetin or polydatin (~20mM) CD3OD solution. Absolute concentrations of both aroma and polyphenols were accurately determined from the surface integration of well-separated 1D 1H signal using qNMR method. Results: 1. The headspace analysis showed that polydatin and quercetin decreased the volatility of IBMP. 2. Compared to the control (IBMP without polyphenol), significant results were found in sensory analysis (triangular tests) for polytadin (P < 0030) and quercetin (P < 0000) in presence of IBMP at 8 ng/L in model wine solution 3. Variations in NMR 1H chemical shifts were observed in titration experiments which suggest different possible sites for the interactions of IBMP with the polyphenols

[1] D. Sidhu, J.Lund, Y. Kotseridis, C.Saucier (2015). Methoxypyrazine Analysis and influence of Viticultural and Enological Procedures on their Levels in Grapes, Musts, and Wines, Critical reviews in Food Science and Nutrition, 55:4, 485-502, [2]. Aronson, J., & Ebeler, S. E. (2004). Effect of polyphenol compounds on the headspace volatility of flavors. American Journal of Enology and Viticulture, 55 :1, 13-21.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Cédric Saucier*, Audrey Bloem, Christine Le Guernevé, Damien Lorenzi, Elizabeth Johansson Filote, Nicolas Bouvier, Peggy Rigou, Veronique Cheynier

*Université de Montpellier

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Removal of Fumonisin B1 and B2 from red wine using polymeric substances

The Ability of PVPP (Polyvinylpolypyrrolidone), PVP-DEGMA-TAIC (copolimerization of N-vinyl-2-pyrrolidinone with ethylene glycol dimethacrylate and triallyl isocyanurate) and PAEGDMA
(poly(acrylamide-co-ethylene glycol dimethacrylate)) polymers was tested as removal agents for Fumonisin B1 (FB1) and Fumonisin B2 (FB2) from model solutions and red wine. The polymers removal capacity was checked at three different resident times (2, 8 and 24 hours of contact time between the polymer and the sample), showing no differences in the percentage of FB1 and FB2 removal. Then, different polymer concentrations (1, 5 and 10 mg mL-1) were tested in model solution with and without phenolics (i.e. gallic acid and 4-methylcatechol).

How pressing techniques affect must composition and wine quality of Pinot blanc

This study investigates how the sensory profile of Pinot Blanc is affected from different maceration and pressing techniques. Grapes were sourced from four vineyards in the village Tramin in South Tyrol. For the experiment 200 kg of grapes from each vineyard site were hand picked the day before harvest for the commercial winery took place. Grapes were stored over night at 4°C, homogenized and processed in the experimental winery at Laimburg research centre the day after harvest. Four different pressing techniques were applied in duplicates of 100kg each.

Comparative proteomic analysis of wines made from Botrytis cinerea infected and healthy grapes reveal interesting parallels to the gushing phenomenon in sparkling wine

In addition to aroma compounds also protein composition strongly influences the quality of wines. Proteins of wine derive mainly from the plant Vitis vinifera and may be influenced by abiotic stress as well as fermentation conditions or fining. Additionally, fungal infections can affect the protein content as well by introducing fungal proteins or affecting grape protein composition. An infection of the vine with the plant pathogenic fungus Botrytis (B.) cinerea was shown to cause a degradation of proteins in the resulting wine. Moreover, it influences the foaming properties in sparkling wine.

Use of chitosan as a secondary antioxidant in juices and wines

Chitosan is a polysaccharide produced from the deacetylation of chitin extracted from crustaceous and fungi. In winemaking chitosan is mainly used in the clarification of grape juice and wine, stabilization of white wines, removal of metals and to prevent wine spoilage by undesired microorganisms. The addition of chitosan to model wine systems was able to retard browning, reduce levels of metallic ions (Fe and Cu) and to protect varietal thiols due to its antiradical activity1. The present experiment was planned in order to evaluate the use of chitosan as a secondary antioxidant at three different stages of Sauvignon blanc fermentation and winemaking. Sauvignon blanc juices from three different locations were obtained at a commercial winery in Marlborough, New Zealand. One lots of grapes was collected from a receival bin and pressed into juice with a water-bag press, and a further juice sample was collected from a commercial pressing operation. Chitosan (1 g/L, low molecular weight, 75 – 85% deacetylated) was added to the juice after pressing, after cold settling, after fermentation, or at all these stages. Controls without any chitosan additions were also prepared.

Monitoring of Pesticide Residues from Vine to Wine

Those previous years, pesticides are often brought to the forefront by media. Questions arose about their toxicity for growers and consumers. Even if a downward trend is underway, the use of pesticides is required to ensure steady quality and quantity of harvests. A large number of active ingredients are authorized but regarding viticulture, mainly insecticides and fungicides are applied, to control pests and diseases and to increase crop yield. Some phytosanitary products, principally fungicides, applied close to the harvest date may frequently be detected in wines.