Macrowine 2021
IVES 9 IVES Conference Series 9 IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

Abstract

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA). The analyses of polyphenols and IBMP in model wine were all performed in 10ml vial flasks with volumes of 1ml of model wine volume. IBMP was quantified in Single Ion Monitoring (SIM) mode. The ions used for SIM mode were m/z 94, 121, 154. All the solutions were at fixed pH and ethanol concentration (pH 3.5, 12% Ethanol). For each experiment a control of IBMP, polyphenol, a blank and a mix between IBMP and polyphenol were prepared. 2. Sensory Analysis: Triangular tests were performed by trained panelists (n=26) to orthonasally differentiate between IBMP on its own and IBMP in the presence of a polyphenol. 3. NMR Spectroscopy : Spectra were performed on an Agilent 500 MHz DD2 NMR spectrometer (Agilent Technologies, Santa Clara, CA, USA).Titrations of polyphenols were obtained by addition of IBMP solubilized in CD3OD (~200mM) to quercetin or polydatin (~20mM) CD3OD solution. Absolute concentrations of both aroma and polyphenols were accurately determined from the surface integration of well-separated 1D 1H signal using qNMR method. Results: 1. The headspace analysis showed that polydatin and quercetin decreased the volatility of IBMP. 2. Compared to the control (IBMP without polyphenol), significant results were found in sensory analysis (triangular tests) for polytadin (P < 0030) and quercetin (P < 0000) in presence of IBMP at 8 ng/L in model wine solution 3. Variations in NMR 1H chemical shifts were observed in titration experiments which suggest different possible sites for the interactions of IBMP with the polyphenols

[1] D. Sidhu, J.Lund, Y. Kotseridis, C.Saucier (2015). Methoxypyrazine Analysis and influence of Viticultural and Enological Procedures on their Levels in Grapes, Musts, and Wines, Critical reviews in Food Science and Nutrition, 55:4, 485-502, [2]. Aronson, J., & Ebeler, S. E. (2004). Effect of polyphenol compounds on the headspace volatility of flavors. American Journal of Enology and Viticulture, 55 :1, 13-21.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Cédric Saucier*, Audrey Bloem, Christine Le Guernevé, Damien Lorenzi, Elizabeth Johansson Filote, Nicolas Bouvier, Peggy Rigou, Veronique Cheynier

*Université de Montpellier

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of nanofiltration on the chemical composition and wine quality

In Enology the conventional processes of filtration for clarification and stabilization are giving place to alternative membrane processes, including nanofiltration (NF). Furthermore, the increased alcohol content in wines recorded in recent years became an important issue for all the main wine producing countries. Among techniques available to the wine industry to reduce the ethanol content, NF is certainly one of the newest. This study is focused on the evaluation of NF influence on wine physical-chemical composition, including mineral content, which in accordance to our best knowledge is a novelty.

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.

Development and validation of a standardized oxidation assay for the accurate measurement of the ability of different wines to form “de novo” oxidation-related aldehydes

From the standpoint of wine aroma oxidation there are two effects observed: aroma degradation of oxygen sensitive compounds (polyfunctional mercaptans) and the appearance of new substances with high aromatic power (acetaldehyde, methional, phenylacetaldehyde, sotolon, alkenals, isobutanal and 2, 3-metylbutanals) (1-5). According to our experience, Strecker aldehydes are compounds with highest sensory relevance in the oxidative degradation of many wines (5-7).

The influence of soil management practices on functional traits and biodiversity of weed communities in Swiss vineyards

Green cover in vine rows provides many ecological services, but can also negatively impact the crop, depending on the weed species. The composition of a vineyard weed community is influenced by many parameters. Ensuring an evolution of the vine row flora into a desired direction is therefore very complex. A key step towards this goal is to know which factors influence the establishment of the weed community and which types of communities are best suited for vineyards. In this study, we analysed the weed communities of several vineyards in the Lake Geneva region (379 botanical surveys on 117 plots), with the aim to highlight the links between soil management practices (chemical and mechanical weeding, mowing, mulching roll) and phytosociological profiles, biodiversity and selected functional traits (growth forms, life strategies, root depth). T

Extraction of polyphenols from grape marc by supercritical fluid extraction (SFE) and evaluation of their ‘bioavailability’ as dietary supplements

In the winemaking process, several compounds that remain in the grape skins and seeds after the fermentation stage are bioactive-compounds (substances with potential beneficial effects on health) that can be extracted in order to recovery valuable substances with a high commercial value for the cosmetic, food (nutraceuticals) and pharmaceutical industries. The skins contain significant amounts of bioactive substances such as tannins (16-27%) and other polyphenolic compounds (2-6.5%) in particular, catechins, anthocyanins, proanthocyanins, quercetin , ellagic acid and resveratrol.