Macrowine 2021
IVES 9 IVES Conference Series 9 IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

Abstract

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA). The analyses of polyphenols and IBMP in model wine were all performed in 10ml vial flasks with volumes of 1ml of model wine volume. IBMP was quantified in Single Ion Monitoring (SIM) mode. The ions used for SIM mode were m/z 94, 121, 154. All the solutions were at fixed pH and ethanol concentration (pH 3.5, 12% Ethanol). For each experiment a control of IBMP, polyphenol, a blank and a mix between IBMP and polyphenol were prepared. 2. Sensory Analysis: Triangular tests were performed by trained panelists (n=26) to orthonasally differentiate between IBMP on its own and IBMP in the presence of a polyphenol. 3. NMR Spectroscopy : Spectra were performed on an Agilent 500 MHz DD2 NMR spectrometer (Agilent Technologies, Santa Clara, CA, USA).Titrations of polyphenols were obtained by addition of IBMP solubilized in CD3OD (~200mM) to quercetin or polydatin (~20mM) CD3OD solution. Absolute concentrations of both aroma and polyphenols were accurately determined from the surface integration of well-separated 1D 1H signal using qNMR method. Results: 1. The headspace analysis showed that polydatin and quercetin decreased the volatility of IBMP. 2. Compared to the control (IBMP without polyphenol), significant results were found in sensory analysis (triangular tests) for polytadin (P < 0030) and quercetin (P < 0000) in presence of IBMP at 8 ng/L in model wine solution 3. Variations in NMR 1H chemical shifts were observed in titration experiments which suggest different possible sites for the interactions of IBMP with the polyphenols

[1] D. Sidhu, J.Lund, Y. Kotseridis, C.Saucier (2015). Methoxypyrazine Analysis and influence of Viticultural and Enological Procedures on their Levels in Grapes, Musts, and Wines, Critical reviews in Food Science and Nutrition, 55:4, 485-502, [2]. Aronson, J., & Ebeler, S. E. (2004). Effect of polyphenol compounds on the headspace volatility of flavors. American Journal of Enology and Viticulture, 55 :1, 13-21.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Cédric Saucier*, Audrey Bloem, Christine Le Guernevé, Damien Lorenzi, Elizabeth Johansson Filote, Nicolas Bouvier, Peggy Rigou, Veronique Cheynier

*Université de Montpellier

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Evaluation of Polarized Projective Mapping as a possible tool for attributing South African Chenin blanc dry wine styles

Multiple Factor Analysis (MFA) According to the Chenin blanc Association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded (RRU), and Rich and Ripe Wooded (RRW), classically attributed with the help of sensory evaluation. One of the “rapid methods” has drawn our attention for the purpose of simplifying and making style attribution for large sample sets, evaluated during different sessions, more robust. Polarized Projective Mapping (PPM) is a hybrid of Projective Mapping (PM) and Polarised Sensory Positioning (PSP). It is a reference-based method in which poles
(references) are used for the evaluation of similarities and dissimilarities between samples.

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

Supramolecular approaches to the study of the astringency elicited by wine phenolic compounds

The objective of this study is to review the scientific evidences and to advance into the knowledge of the molecular mechanisms of astringency. Astringency has been described as the drying, roughing and puckering sensation perceived when some food and beverages are tasted (1). The main, but possibly not the only, mechanism for the astringency is the precipitation of salivary proteins (2,3). Between phenolic compounds found in red wines, flavan-3-ols are the group usually related to the development of this sensation. Other compounds, phenolic or not, like anthocyanins, polysaccharides and mannoproteins could act modifying or modulating astringency perception by hindering the interaction between flavanols and salivary proteins either because of their interaction with the flavanols or because of their interaction with the salivary proteins.

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.

Identification of green, aggressive and hard character of wines by a chemo-sensory directed methodology

With climate change, it is progressively more often to obtain grapes with an acceptable content in sugars or acids but with immature tannins described as green, aggressive or hard (noted as GAH onwards). During winemaking, the oenologist has to make decisions related to the elaboration of such grapes based mainly on empirical experience, given the lack of objective criteria to this concern. An increase in the chemical and sensory knowledge of immature tannins would allow managing this GAH character of grapes with the maximum possible efficiency during winemaking processes. The present work aims at isolating and identifying the group of compounds responsible for the GAH character present in wines.