Macrowine 2021
IVES 9 IVES Conference Series 9 How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

Abstract

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method. The experimental design included 12 different wines from five different cultivars. The wines (n=12×20) were bottled at the same industrial bottling line, then stored for 60 days at room temperature. Half of the bottles were filled using the standard process with inert headspace, and sealed with a synthetic coextruded stopper allowing lower oxygen ingress, resulting in a total package oxygen (TPO) in the range 1.30 – 4.25 ppm O2. The other half of the bottles were filled without inert gas and with extra headspace, and sealed with a synthetic coextruded stopper allowing higher oxygen ingress, resulting in TPO 5.93 – 8.38 ppm O2. After storage, the wines were analysed using an untargeted LC-ESI-QTOF MS method, optimised for wine metabolomics, to obtain the widest coverage of the metabolic space of non-volatiles [1]. This experiment produced a dataset with over 20,000 features, and data analysis showed the presence of about 35 putative markers induced by different amounts of oxygen. These metabolite markers included ascorbic acid, tartaric acid and various sulfonated compounds. Thus, the antioxidant SO2 takes part in various reactions, modulated by the presence of oxygen, several of which were unknown in wine to date and would appear to be of practical significance. Specifically, the sulfonated derivatives of indole-3-lactic hexoside, tryptophol, glutathione, cysteine and pantetheine were detected in wine for the first time, thanks to the untargeted metabolomics approach chosen. These findings explains why glutathione disulfide is not detectable in wines, due to its preferential antagonistic reaction with SO2. Further studies of the mechanisms involved in such reactions and the inclusion of selected SO2-binding compounds in the routinely quality control of wines could help to decrease SO2 addition in wine, and make smarter use of the various oenological antioxidants in correlation with varietal information, the amount of total package oxygen and the choice of stopper. Acknowledgments The authors thank Nomacorc for its financial support and the MezzaCorona winery for the wines, bottling and storage.

Reference [1] Arapitsas, P. et al., Journal of Chromatography A, 2016, 1429, 155-165

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Fulvio Mattivi*, Andrea Angeli, Daniele Perenzoni, Maurizio Ugliano, Panagiotis Arapitsas, Paolo Pangrazzi

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Evaluation of Polarized Projective Mapping as a possible tool for attributing South African Chenin blanc dry wine styles

Multiple Factor Analysis (MFA) According to the Chenin blanc Association of South Africa, there are three recognized dry wine styles, Fresh and Fruity (FF), Rich and Ripe Unwooded (RRU), and Rich and Ripe Wooded (RRW), classically attributed with the help of sensory evaluation. One of the “rapid methods” has drawn our attention for the purpose of simplifying and making style attribution for large sample sets, evaluated during different sessions, more robust. Polarized Projective Mapping (PPM) is a hybrid of Projective Mapping (PM) and Polarised Sensory Positioning (PSP). It is a reference-based method in which poles
(references) are used for the evaluation of similarities and dissimilarities between samples.

Analysis of voltammetric fingerprints of different white grape musts reveals genotype-related oxidation patterns

Must oxidation is a complex process involving multiple enzymatic transformations, including the oxidation of phenolics containing an ortho-diphenol function. The latter process has a primary influence on wine aroma characteristics and stability, due to the central role of ortho-diphenols in the non-enzymatic oxidative reactions taking place during winemaking and in finished wine. Although oxidation of must is traditionally avoided, in recent years its contribution to wine quality has been revisited, and in some cases improvements to wine aroma have been observed with the application of controlled must oxidation. Nowadays there is a great interest in the wine industry towards the identification of specific markers or patterns to characterize and classify the response of grape must to oxidation.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”.

Influence of inactive dry yeast treatments during grape ripening on postharvest berry skin texture parameters and phenolic compounds extractability

Inactive dry yeast treatments in the vineyard are a tool used with the aim to improve the concentration and quality of secondary metabolites in grapes, leading to a better differentiation of the wines made from grapes differently treated. In this work, a foliar spraying treatment with yeast derivatives specifically designed to be used with the patent pending application technology of Lallemand Inc. Canada (LalVigne® Mature, Lallemand Inc., Montreal, Canada) was tested on Vitis vinifera L. cv. Barbera and Nebbiolo black winegrapes. The aim was to evaluate the effect of this treatment on the phenolic compounds accumulation, the skin physical-mechanical properties and the related phenolic extractability. Prior to analysis, the berries were sorted by flotation in order to evaluate their distribution by density class, and to determine the skin texture parameters of berries with different sugar contents, thus understanding also the ripening effect.