Macrowine 2021
IVES 9 IVES Conference Series 9 How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

Abstract

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method. The experimental design included 12 different wines from five different cultivars. The wines (n=12×20) were bottled at the same industrial bottling line, then stored for 60 days at room temperature. Half of the bottles were filled using the standard process with inert headspace, and sealed with a synthetic coextruded stopper allowing lower oxygen ingress, resulting in a total package oxygen (TPO) in the range 1.30 – 4.25 ppm O2. The other half of the bottles were filled without inert gas and with extra headspace, and sealed with a synthetic coextruded stopper allowing higher oxygen ingress, resulting in TPO 5.93 – 8.38 ppm O2. After storage, the wines were analysed using an untargeted LC-ESI-QTOF MS method, optimised for wine metabolomics, to obtain the widest coverage of the metabolic space of non-volatiles [1]. This experiment produced a dataset with over 20,000 features, and data analysis showed the presence of about 35 putative markers induced by different amounts of oxygen. These metabolite markers included ascorbic acid, tartaric acid and various sulfonated compounds. Thus, the antioxidant SO2 takes part in various reactions, modulated by the presence of oxygen, several of which were unknown in wine to date and would appear to be of practical significance. Specifically, the sulfonated derivatives of indole-3-lactic hexoside, tryptophol, glutathione, cysteine and pantetheine were detected in wine for the first time, thanks to the untargeted metabolomics approach chosen. These findings explains why glutathione disulfide is not detectable in wines, due to its preferential antagonistic reaction with SO2. Further studies of the mechanisms involved in such reactions and the inclusion of selected SO2-binding compounds in the routinely quality control of wines could help to decrease SO2 addition in wine, and make smarter use of the various oenological antioxidants in correlation with varietal information, the amount of total package oxygen and the choice of stopper. Acknowledgments The authors thank Nomacorc for its financial support and the MezzaCorona winery for the wines, bottling and storage.

Reference [1] Arapitsas, P. et al., Journal of Chromatography A, 2016, 1429, 155-165

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Fulvio Mattivi*, Andrea Angeli, Daniele Perenzoni, Maurizio Ugliano, Panagiotis Arapitsas, Paolo Pangrazzi

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Grape byproducts as source of resveratrol oligomers for the development of antifungal extracts

Grape canes are a non-recycled byproduct of wine industry (1-5 tons per hectare per year) containing valuable phytochemicals of medicine and agronomical interest. Resveratrol and wine polyphenols are known to exert a plethora of health-promoting effects including antioxidant capacity, cardioprotection, anticancer activity, anti-inflammatory effects, and estrogenic/antiestrogenic properties (Guerrero et al. 2009). Additionally, resveratrol is a major phytoalexin produced by plants in response to various stresses and promotes disease resistance (Chang et al. 2011). Our project aims to develop polyphenol-rich grape cane extracts to fight phytopathogenic or clinically relevant fungi. We initiate the project with the development of analytical methods to analyze resveratrol mono- and oligomers (dimers, trimers and tetramers) from grape canes and we evaluate their potential activity against clinically relevant opportunistic fungal pathogens (Houillé et al. 2014).

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

Comparison of fortified, sfursat and passito winemaking techniques for the enhancement of the oenological potential of the black grape cultivar Moscato nero d’Acqui (Vitis vinifera L.)

One of the key factors of the economical development of viticulture and wine industry in specific limited areas is the exploitation of ancient, local grape varieties. Therefore, in recent years the growing interest to rediscover minor varieties, previously cultivated, has promoted many studies. With this regard, the focus of this study was the Vitis vinifera L. cultivar Moscato nero d’Acqui, nowadays found only in old vineyards in the Acqui zone (North-West Italy). In particular, the aims of this work were: i) to investigate secondary metabolites profile of the grapes, and ii) to evaluate the attitude to the production of special wines.

Moscatel vine-shoot extracts as grapevine biostimulant to increase the varietal aroma of Airén wines

There is a growing interest in the exploitation of vine-shoots waste, since they are often left or burned. Sánchez-Gómez et al. [1] have shown that vines-shoots aqueous extracts have significant contents of bioactive compounds, among which several polyphenols and volatiles are highlighted. Recent studied had demonstrated that the chemical composition of vine-shoots is enhanced when vine-shoots are toasted
[2,3]. The application of vegetable products in the vineyards has led to significant changes towards a more “Sustainable Viticulture”. An innovative foliar application for Airén vine-shoot extracts have been carried out to the vineyard. It has been shown that they act as grape biostimulants, improving certain wine quality characteristics [4].

Analysis of the oenological potentials of different oak forests in Hungary

Like France, Hungary has many oak forests used for making barrels since many years. But if the differences between the woods of the North, the East and the South-West forests of France are well known, this is probably not the case of Hungarian forests. However taking into account the essential differences of climates and soils, differences must be significant and the general name “Hungarian oak” must not have any real meaning. We have studied precisely (determination of concentrations of volatile and non-volatile wood compounds, anatomical criteria, measurement of antioxidant capacity) of oaks collected from northeastern Hungary and others collected from the Danube valley in the northwest of the country.