Macrowine 2021
IVES 9 IVES Conference Series 9 How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

Abstract

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method. The experimental design included 12 different wines from five different cultivars. The wines (n=12×20) were bottled at the same industrial bottling line, then stored for 60 days at room temperature. Half of the bottles were filled using the standard process with inert headspace, and sealed with a synthetic coextruded stopper allowing lower oxygen ingress, resulting in a total package oxygen (TPO) in the range 1.30 – 4.25 ppm O2. The other half of the bottles were filled without inert gas and with extra headspace, and sealed with a synthetic coextruded stopper allowing higher oxygen ingress, resulting in TPO 5.93 – 8.38 ppm O2. After storage, the wines were analysed using an untargeted LC-ESI-QTOF MS method, optimised for wine metabolomics, to obtain the widest coverage of the metabolic space of non-volatiles [1]. This experiment produced a dataset with over 20,000 features, and data analysis showed the presence of about 35 putative markers induced by different amounts of oxygen. These metabolite markers included ascorbic acid, tartaric acid and various sulfonated compounds. Thus, the antioxidant SO2 takes part in various reactions, modulated by the presence of oxygen, several of which were unknown in wine to date and would appear to be of practical significance. Specifically, the sulfonated derivatives of indole-3-lactic hexoside, tryptophol, glutathione, cysteine and pantetheine were detected in wine for the first time, thanks to the untargeted metabolomics approach chosen. These findings explains why glutathione disulfide is not detectable in wines, due to its preferential antagonistic reaction with SO2. Further studies of the mechanisms involved in such reactions and the inclusion of selected SO2-binding compounds in the routinely quality control of wines could help to decrease SO2 addition in wine, and make smarter use of the various oenological antioxidants in correlation with varietal information, the amount of total package oxygen and the choice of stopper. Acknowledgments The authors thank Nomacorc for its financial support and the MezzaCorona winery for the wines, bottling and storage.

Reference [1] Arapitsas, P. et al., Journal of Chromatography A, 2016, 1429, 155-165

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Fulvio Mattivi*, Andrea Angeli, Daniele Perenzoni, Maurizio Ugliano, Panagiotis Arapitsas, Paolo Pangrazzi

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Field-grown Sauvignon Blanc berries react to increased exposure by controlling antioxidant homeostasis and displaying UV acclimation responses that are influenced by the level of ambient light

Leaf removal in the bunch zone is a common viticultural practice with several objectives, yet it has been difficult to conclusively link the physiological mechanism(s) and metabolic berry impact to this widely practiced treatment. We used a field-omics approach1 in a Sauvignon blanc high altitude model vineyard, showing that the early leaf removal in the bunch zone caused quantifiable and stable responses (over years) in the microclimate where the main perturbation was increased exposure. We provide an explanation for how leaf removal leads to the shifts in grape metabolites typically linked to this treatment and confirm anecdotal evidence and previous reports that leaf removal treatment at an early stage of berry development affects “quality-associated” metabolites (monoterpenes and norisoprenoids).

Oak wood seasoning: impact on oak wood chemical composition and sensory quality of wine

Oak wood selection and maturation are essential steps in the course of barrel fabrication. Given the existence of many factors involved in the choice of raw material and in natural seasoning of oak wood, it is very difficult to determine the real impact of seasoning and selection factors on oak wood composition. A sampling was done to study the evolution of oak wood chemical composition during four seasoning steps: non matured, 12 months, 18 months and 24 months. For this sampling, three selection factors were taken into account: age, grain type and the Polyphenolic Index measured by Oakscan®. Besides extractables
(~10%), three polymers constitute the main part of oak wood: cellulose, hemicelluloses and lignins.

Interest and impact of PVP/PVI (Polyvinylpyrrolidone/ Polyvinylimidazole) on winemaking and final quality of wines

Céline Sparrow a, Christophe Morge a, a SOFRALAB SAS, 79, av. A.A. Thévenet – CS 11031 – 51530 Magenta, France Consumers’ health and security force authorities to limit, in wine as in others food industry products, the concentration in « dangerous » molecules. Therefore the legal limit in heavy metals keeps on decreasing. As per proof EU regulation just decrease the stain concentration in wine from 0,2 to 0,15 mg/l. Certain changes , such as sodium arsenite treatment in vines, disappearance of brass in wineries to the benefit of stainless steel, limit even more the concentration of heavy metals in wines. But the use of copper derivates in vines treatments is difficult to replace. In the case of wine and its elaboration, the problem is even more complex. Indeed, regulation forces the wine producers to control the concentration of certain heavy metals in final wines.

Oxygen consumption by diferent oenological tanins in a model wine solution

INTRODUCTION: Oenological tannins are widely used in winemaking to improve some characteristics of wines [1] being the antioxidant properties probably one of the main reasons [2]. However, commercial tannins have different botanical sources and chemical composition [3] which probably determines different antioxidant potential. There are some few references about the antioxidant properties of commercial tannins [4] but none of them have really measured the direct oxygen consumption by them. The aim of this work was to measure the kinetics of oxygen consumption by different commercial tannins in order to determine their real capacities to protect wine against oxygen. MATERIAL AND METHODS: 4 different commercial tannins were used: T1: condensed tannin from grape seeds, T2: gallotannin from chinese gallnuts, T3: ellagitannin from oak and T4: tannin from quebracho containing condensed tannins and ellagitannins.

Capture depletion of grapevine DNA: an approach to advance the study of microbial community in wine

The use of next-generation sequencing (NGS) has helped understand microbial genetics in oenology. Current studies mainly focus on barcoded amplicon NGS but not shotgun sequencing, which is useful for functional analyses. Since the high percentage of grapevine DNA conceals the microbial DNA in must, the majority of sequencing data is wasted in bioinformatic analyses. Here we present capture depletion of grapevine whole genome DNA.