Macrowine 2021
IVES 9 IVES Conference Series 9 How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

Abstract

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method. The experimental design included 12 different wines from five different cultivars. The wines (n=12×20) were bottled at the same industrial bottling line, then stored for 60 days at room temperature. Half of the bottles were filled using the standard process with inert headspace, and sealed with a synthetic coextruded stopper allowing lower oxygen ingress, resulting in a total package oxygen (TPO) in the range 1.30 – 4.25 ppm O2. The other half of the bottles were filled without inert gas and with extra headspace, and sealed with a synthetic coextruded stopper allowing higher oxygen ingress, resulting in TPO 5.93 – 8.38 ppm O2. After storage, the wines were analysed using an untargeted LC-ESI-QTOF MS method, optimised for wine metabolomics, to obtain the widest coverage of the metabolic space of non-volatiles [1]. This experiment produced a dataset with over 20,000 features, and data analysis showed the presence of about 35 putative markers induced by different amounts of oxygen. These metabolite markers included ascorbic acid, tartaric acid and various sulfonated compounds. Thus, the antioxidant SO2 takes part in various reactions, modulated by the presence of oxygen, several of which were unknown in wine to date and would appear to be of practical significance. Specifically, the sulfonated derivatives of indole-3-lactic hexoside, tryptophol, glutathione, cysteine and pantetheine were detected in wine for the first time, thanks to the untargeted metabolomics approach chosen. These findings explains why glutathione disulfide is not detectable in wines, due to its preferential antagonistic reaction with SO2. Further studies of the mechanisms involved in such reactions and the inclusion of selected SO2-binding compounds in the routinely quality control of wines could help to decrease SO2 addition in wine, and make smarter use of the various oenological antioxidants in correlation with varietal information, the amount of total package oxygen and the choice of stopper. Acknowledgments The authors thank Nomacorc for its financial support and the MezzaCorona winery for the wines, bottling and storage.

Reference [1] Arapitsas, P. et al., Journal of Chromatography A, 2016, 1429, 155-165

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Fulvio Mattivi*, Andrea Angeli, Daniele Perenzoni, Maurizio Ugliano, Panagiotis Arapitsas, Paolo Pangrazzi

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Assessing the effect of oak derived aromas on mouthfeel perception in Chardonnay wine

Mouthfeel is an important quality parameter for Chardonnay wines, particularly those aged in oak. While research on mouthfeel has traditionally focused on the impact of non-aromatic compounds, the role of aroma compounds has largely been over looked. However, in wine as well as other food interactions between retronasal aroma and mouthfeel have been noted. The goal of this research was to investigate the impact of wine aroma on the perception of mouthfeel. Because of the importance of oak aging in the development of Chardonnay mouthfeel, the impact of oak aromas on perceived mouthfeel was explored. Aroma compounds associated with oak (ethyl palmitate, eugenol, furfural, isoeugenol, syringaldehyde, vanillin and whiskey lactone) were added to two different Chardonnay wines; one with no oak influence and one fermented in neutral oak. Low and high concentrations of the compounds were added based on concentrations typically found in barrel aged Chardonnay wine.

Chemical markers in wine related to low levels of yeast available nitrogen in the grape

Nitrogen is an important nutrient of yeast and its low content in grape must is a major cause for sluggish fermentations. To prevent problems during fermentation, a supplementation of the must with ammonium salts or more complex nitrogen mixtures is practiced in the cellar. However this correction seems to improve only partially the quality of wine [1]. In fact, yeast is using nitrogen in many of its metabolic pathways and depending of the sort of the nitrogen source (ammonium or amino acids) it produces different flavor active compounds. A limitation in amino acids can lead to a change in the metabolic pathways of yeast and consequently alter wine quality.

Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Fermentative aromas (especially esters and higher alcohols) highly impact the organoleptic profile of young and white wines. The production of these volatile compounds depends mainly on temperature and Yeast Available Nitrogen (YAN) content in the must. Available dynamic models predict the main reaction
(bioconversion of sugar into ethanol and CO2 production) but none of them considers the production kinetics of fermentative aroma compounds during the process of fermentation. We determined the production kinetics of the main esters and higher alcohols for different values of initial YAN content and temperature, using an innovative online monitoring Gas Chromatography device.

Extraction of pathogenesis-related proteins and phenolics in Sauvignon Blanc as affected by different

The composition of wine is largely determined by the composition of pre-fermentation juice, which is influenced by extraction of grape components. Different grape harvesting and processing conditions could affect the extraction of grape components into juice. Among these grape components, pathogenesis-related (PR) proteins are of great concern for white wine maker as they are the main cause of haze formation in finished white wine. If not removed before bottling, these PR proteins may progress into haze through the formation of complex with phenolics under certain conditions. Thaumatin-like proteins (TLPs) and chitinases are the main constituents of PR proteins found in protein haze.

Effects of a new vacuum evaporation method on chemical and sensory properties of must and wine

A new process for vacuum evaporation was developed where evaporation takes place near the inner surface of a vortex produced by a rotor submerged in the liquid. Contrary to the state of the art the Vortex rotor process does not need a vacuum vessel but the rotating liquid creates a geometrically stable low pressure void surrounded by a vortex stabilized by the equilibrium between centrifugal forces and the pressure difference. First tests with water and sugar solutions at concentrations similar to grape must were conducted to verify the theoretical predictions, test the performance under different conditions and study the effect of various process parameters (Rösti et al 2015).