Macrowine 2021
IVES 9 IVES Conference Series 9 How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

How small amounts of oxygen introduced during bottling and storage can influence the metabolic fingerprint and SO2 content of white wines

Abstract

The impact of minute amounts of headspace oxygen on the post-bottling development of wine is generally considered to be very important, since oxygen, packaging and storage conditions can either damage or improve wine quality. This is reflected in the generalised use of inert bottling lines, where the headspace between the white wine and the stopper is filled with an inert gas. This experiment aimed to address some open questions about the chemistry of the interaction between wine and oxygen, crucial for decisions regarding optimal closure. While it is known that similar amounts of oxygen affect different wines to a variable extent, our knowledge of chemistry is not sufficient to construct a predictive method. The experimental design included 12 different wines from five different cultivars. The wines (n=12×20) were bottled at the same industrial bottling line, then stored for 60 days at room temperature. Half of the bottles were filled using the standard process with inert headspace, and sealed with a synthetic coextruded stopper allowing lower oxygen ingress, resulting in a total package oxygen (TPO) in the range 1.30 – 4.25 ppm O2. The other half of the bottles were filled without inert gas and with extra headspace, and sealed with a synthetic coextruded stopper allowing higher oxygen ingress, resulting in TPO 5.93 – 8.38 ppm O2. After storage, the wines were analysed using an untargeted LC-ESI-QTOF MS method, optimised for wine metabolomics, to obtain the widest coverage of the metabolic space of non-volatiles [1]. This experiment produced a dataset with over 20,000 features, and data analysis showed the presence of about 35 putative markers induced by different amounts of oxygen. These metabolite markers included ascorbic acid, tartaric acid and various sulfonated compounds. Thus, the antioxidant SO2 takes part in various reactions, modulated by the presence of oxygen, several of which were unknown in wine to date and would appear to be of practical significance. Specifically, the sulfonated derivatives of indole-3-lactic hexoside, tryptophol, glutathione, cysteine and pantetheine were detected in wine for the first time, thanks to the untargeted metabolomics approach chosen. These findings explains why glutathione disulfide is not detectable in wines, due to its preferential antagonistic reaction with SO2. Further studies of the mechanisms involved in such reactions and the inclusion of selected SO2-binding compounds in the routinely quality control of wines could help to decrease SO2 addition in wine, and make smarter use of the various oenological antioxidants in correlation with varietal information, the amount of total package oxygen and the choice of stopper. Acknowledgments The authors thank Nomacorc for its financial support and the MezzaCorona winery for the wines, bottling and storage.

Reference [1] Arapitsas, P. et al., Journal of Chromatography A, 2016, 1429, 155-165

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Fulvio Mattivi*, Andrea Angeli, Daniele Perenzoni, Maurizio Ugliano, Panagiotis Arapitsas, Paolo Pangrazzi

*Fondazione Edmund Mach

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

Full automation of oenological fermentations and its application to the processing of must containing high sugar or acetic acid concentrations

Climate change and harvest date decisions have led to the evolution of must quality over the last decades. Increases in must sugar concentrations are among the most obvious consequences, quantitatively. Saccharomyces cerevisiae is a robust and acid tolerant organism. These properties, its sugar to ethanol conversion rate and ethanol tolerance make it the ideal production organism for wine fermentations. Unfortunately, high sugar concentrations may affect S. cerevisiae and lead to growth inhibition or yeast lysis, and cause sluggish or stuck fermentations. Even sublethal conditions cause a hyperosmotic stress response in S. cerevisiae which leads to increased formation of fermentation by-products, including acetic acid, which may exceed legal limits in some wines.

Impact of non-fruity compounds on red wines fruity aromatic expression: the role of higher alcohols

A part, at least, of the fruity aroma of red wines is the consequence of perceptive interactions between various aromatic compounds, particularly ethyl esters and acetates, which may contribute to the perception of fruity aromas, specifically thanks to synergistic effects.1,2 The question of the indirect impact of non-fruity compounds on this particular aromatic expression has not yet been widely investigated. Among these compounds higher alcohols (HA) represent the main group, from a quantitative standpoint, of volatiles in many alcoholic beverages. Moreover, some bibliographic data suggested their contribution to the aromatic complexity by either increasing or masking flavors of wine, depending of their concentrations.

Microbial life in the grapevine: what can we expect from the leaf microbiome?

The above-ground parts of plants, which constitute the phyllosphere, have long been considered devoid of bacteria and fungi, at least in their internal tissues and microbial presence there was long considered a sign of disease. However, recent studies have shown that plants harbour complex bacterial communities, the so-called “microbiome”[1]. We are only beginning to unravel the origin of these bacterial plant inhabitants, their community structure and their roles, which in analogy to the gut microbiome, are likely to be of essential nature. Among their multifaceted metabolic possibilities, bacteria have been recently demonstrated to emit a wide range of volatile organic compounds (VOCs), which can greatly impact the growth and development of both the plant and its disease-causing agents.

Use of computational modelling for selecting adsorbents for improved fining of wine

The occurrence of faults and taints in wine, such as those caused by microbial spoilage or various taints, have resulted in significant financial losses to wine producers. The wine industry commits significant financial resources towards fining and taint removal processes each year. Fining involves the addition of one or more adsorptive substrates to juice or wine to bind certain components, thus reducing their concentration [1]. However, these processes are often not selective and can also remove desirable flavour and aroma compounds.