Macrowine 2021
IVES 9 IVES Conference Series 9 HEAT BERRY : Sensitivity of berries ripening to higher temperature and impact on phenolic compounds in wine

HEAT BERRY : Sensitivity of berries ripening to higher temperature and impact on phenolic compounds in wine

Abstract

The grapevine is an important economical crop that is very sensitive to climate changes and microclimate. The observations made during the last decades at a vineyard scale all concur to show the impact of climate change on vine physiology, resulting in accelerated phenology and earlier harvest (Jones and Davis 2000). It is well-known that berry content is affected by the ambient temperature. While the first experiences were primarily conducted on the impact of temperature on anthocyanin accumulation in the grape, few studies have focused on others component of phenolic metabolism, such as tannins. Due to their chemical structure they are involved in the sensation of astringency / bitterness and play a key role in the quality of wines (Downey, Dokoozlian, and Krstic 2006). In a context of climate change and global warming, it is necessary to ask the question of the effect of temperature on the metabolism and its impact on wine quality. One of the goals of the HEAT BERRY project is to understand the physiological basis of the response of secondary metabolism to heat and microclimate, in grape berries, and their resulting effects on wine chemistry and organoleptic properties. A passive heating system made of polycarbonate screens has been set up at the vineyard. Field experiments conducted on Cabernet Sauvignon showed that this system induces 1 to 3°C increase in berry temperature. The heating system was set up in June at fruit set, and berries juices were sampled and used for ripening analysis between veraison and harvest time. The results of ripening agreed with previous studies: phenolic maturity and extractability of anthocyanins were decreased on heated berries. Nowadays, it is well-known that tannins nature within different berry tissues has an impact on bitterness and astringency (skins tannins mainly participate to the sensation of astringency, while seeds tannins also contribute to bitterness) as well as the percentage of each berry part. But nothing was shown about the importance of a temperature increase on these aspects. Microvinifications were performed on ripe (and over-ripe) berries. First, tastings demonstrated significant differences between wines from heated berries and non-heated berries in accordance with ripening process and berry compounds. Second, phenolics compounds (tannins levels, Dpm, tannins composition) were analyzed in berries and in wines. The analytical results will be compared with the sensory analysis in order to better understand the qualitative impact of heat treatment on berries and wine properties. Overall, the aim of HEAT BERRY is to address the poor knowledge of the effects of high temperatures on berry composition and wines. This should provide useful clues for the adaptation of viticulture to climate change.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Article

Authors

Julie Drappier*, Cécile Thibon, Darriet Philippe, Jing Wu, Laurence Geny-Denis, Michael Jourdes, Philippe Pieri, Robin Rabagliato, Serge Delrot

*ISVV

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Effect of intra‐vineyard ripeness variation on the efficiency of commercial enzymes on berry cell wall deconstruction under winemaking conditions

Intra-vineyard variation grape berry ripening occurs within bunches, between bunches on the same vine and between vines. Although it is assumed that such variation also occurs at the grape berry cell wall level, no study to data has investigated in any depth. Here we have used a intra-vineyard panel design to investigate pooled bunches from six vines (per panel) in the context of a winemaking scenario. The dissected vineyard was harvested by separate panels, where each panel was then subjected to a standard winemaking procedure with or without the addition of three different enzyme preparations for maceration.

Prediction of the production kinetics of the main fermentative aromas in alcoholic fermentation

Fermentative aromas (especially esters and higher alcohols) highly impact the organoleptic profile of young and white wines. The production of these volatile compounds depends mainly on temperature and Yeast Available Nitrogen (YAN) content in the must. Available dynamic models predict the main reaction
(bioconversion of sugar into ethanol and CO2 production) but none of them considers the production kinetics of fermentative aroma compounds during the process of fermentation. We determined the production kinetics of the main esters and higher alcohols for different values of initial YAN content and temperature, using an innovative online monitoring Gas Chromatography device.

A multivariate approach using attenuated total reflectance mid-infrared spectroscopy to measure the surface mannoproteins and β-glucans of yeast cell walls during wine fermentations

Yeast cells possess a cell wall comprising primarily glycoproteins, mannans, and glucan polymers. Several yeast phenotypes relevant for fermentation, wine processing, and wine quality are correlated with cell wall properties. To investigate the effect of wine fermentation on cell wall composition, a study was performed using mid-infrared (MIR) spectroscopy coupled with multivariate methods (i.e., PCA and OPLS-DA). A total of 40 yeast strains were evaluated, including Saccharomyces strains (laboratory and industrial) and non-Saccharomyces species. Cells were fermented in both synthetic MS300 and Chardonnay grape must to stationery phase, processed, and scanned in the MIR spectrum.

Using combinations of recombinant pectinases to elucidate the deconstruction of the polysaccharide‐rich grape cell wall during winemaking

The effectiveness of enzyme-mediated maceration processes in red winemaking relies on a clear picture of the target (berry cell wall structure) to achieve the optimum combination of specific enzymes to be used. However, we lack the information on both essential factors of the reaction (i.e. specific activities in commercial enzyme preparation and the cell wall structure of berry tissue). In this study, the different combinations of pure recombinant enzymes and the recently validated high throughput cell wall profiling tools were applied to extend our knowledge on the grape berry cell wall polymeric deconstruction during the winemaking following a combinatorial enzyme treatment design.

Glutathione content evolution during spontaneous alcoholic fermentations of Sangiovese grapes

Glutathione is a tripeptide (γ-Glu-Cys-Gly), which can occur in grapes, in must and in wine prevalently in the reduced form as well as in the oxidized form as glutathione disulfide. The importance of the reduced form of glutathione lies in its antioxidant activity. In must, it limits browning by reducing o-quinones produced by polyphenol oxidase activity on hydroxycinnamic acids; in wine, it exerts a protective effect on various aromatic compounds. Glutathione concentration in wine is lower than in grape juice and variable as it depends on several factors, ranging from the native content of grapes to winemaking technique.