Macrowine 2021
IVES 9 IVES Conference Series 9 Identification of caffeic acid as a major component of Moscatel wine protein sediment

Identification of caffeic acid as a major component of Moscatel wine protein sediment

Abstract

Proteins play a significant role in the colloidal stability and clarity of white wines [1]. However, under conditions of high temperatures during storage or transportation, the proteins themselves can self-aggregate into light-dispersing particles causing the so-called protein haze [2]. Formation of these unattractive precipitates in bottled wine is a common defect of commercial wines, making them unacceptable for sale [3]. Previous studies identified the presence of phenolic compounds in the natural precipitate of white wine [4], contributing to the hypothesis that these compounds could be involved in the mechanism of protein haze formation. The objective of this study was to isolate and identify the compounds contained in induced wine protein haze precipitate after alkaline hydrolysis. The heat-induced protein precipitate from five liters of white Moscatel of Alexandria wine was subjected to alkaline hydrolysis in 2 M NaOH, 10 mM EDTA and 1% (w/v) L-ascorbic acid following a protocol described elsewhere [5] with some modifications. The alkaline hydrolyzed sample was subjected to liquid-liquid extraction with ethyl acetate and evaporated to dryness. The extract was further fractionated using reversed phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD). The major compound present was found to be caffeic acid amongst other minor, unidentified compounds. Caffeic acid was identified by UV-vis spectra and the structure validated by 1H nuclear magnetic resonance (NMR). This work corroborates the observation that phenolic compounds, and caffeic acid in particular, may participate in wine protein haze formation since it is the major compound nonprotein compound present in Moscatel wine protein sediment.

References: [1] F.X. Sauvage, B. Bach, M. Moutounet, and A. Vernhet, Food Chemistry, 2010, 118, 26-34. [2] E.J. Waters, W. Wallace, and P.J. Williams, Journal of Agricultural and Food Chemistry, 1992, 40, 1514-1519. [3] G. Tabilo-Munizaga, T.A. Gordon, R. Villalobos-Carvajal, L. Moreno-Osorio, F.N. Salazar, M. Perez-Won, and S. Acuna, Food Chemistry , 2014, 155, 214-220. [4] M. Esteruelas, N. Kontoudakis, M. Gil, M.F. Fort, J.M. Canals, and F. Zamora, Food Research International, 2011, 44, 77-83. [5] Nardini, M., E. Cirillo, F. Natella, and C. Scaccini, Journal of Agricultural and Food Chemistry, 2002, 50, 5735-5741.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ricardo Chagas*, Ana Lourenço, Luísa Carvalho, Ricardo Ferreira, Sara Monteiro

*FCT/UNL

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

IBMP-Polypenol interactions: Impact on volatility and sensory perception in model wine solution

3-Isobutyl-2-methoxypyrazine (IBMP) is one of the key molecules in wine aroma with a bell pepper aroma and a very low threshold in wine, 1-6 ng/L for white wine and 10-16 ng/L in red wine1. The differences in these thresholds are likely due to IBMP-non volatile matrix interactions. It has indeed been shown that polyphenols may influence the volatility of flavor compounds2. In the present study, we focus on IBMP-polyphenols interactions in relation to volatility and sensory perception in model wine solution. Methods: 1. GC-MS Static Headspace Analysis: Samples were analyzed by Static headspace analysis with an Agilent 7890A gas chromatograph coupled to HP 5975C mass spectrometry detector (Agilent Technologies, Santa Clara, CA, USA).

Prevention of wine oxidation during barrel aging: an innovative method to measure antioxidant

Wine oxidation is a problem that affects the freshness, the aromatic profile, the colour and also the mouthfeel of the wine. It mainly concerns white wines. Oxygen interactions with wine compounds lead to the phenomena cited above that are responsible for the depreciation of these wines. Barrel aging is a crucial step in the wine process because it allows many modifications as wine enrichment, colour stabilization, clarification and also a slow oxygenation of the wine. Effects of the oak barrel have to be known to prevent oxidation of the wine. We have been interested in the main antioxidant compounds released by oak barrels to the wine and we have developed an innovative method to reach directly these antioxidant compounds at the oak stave surface.

Fractionation of copper and iron in wine: Assessment of potential macromolecule and sulfur binding agents

Copper and iron are known to substantially impact wine stability through oxidative, reductive or colloidal phenomena. However, the binding of metal ions to different wine components under wine conditions, and the impact of this binding on the ability of the metal ions to induce spoilage processes, is not well understood. This study surveyed a range of red and white wines for an understanding of the variability of broad metal categories within the wines. The techniques utilized included an electrochemical constant current stripping potentiometry technique (ccSP), and solid phase extraction (SPE) fractionation of wine with subsequent analysis of the metal content of each fraction by inductively coupled plasma – optical emission spectroscopy (ICP-OES).

Fingerprinting the origin of rosé wines with a new high throughput polyphenomics method

Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002[1]. But because of its high commercial value, it can become a subject of fraud, and authenticity control is necessarily required. More than one hundred polyphenols have been recently quantified in various rosé wines [2]. They are key components defining color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, winemaking and age of the wine. In this study, the influence of geographic origin of some rosé French wines was investigated. An original and very fast UPLC-QTOF-MS method was developed and used to predict the geographic origin authenticity of rosé wines.