Macrowine 2021
IVES 9 IVES Conference Series 9 Identification of caffeic acid as a major component of Moscatel wine protein sediment

Identification of caffeic acid as a major component of Moscatel wine protein sediment

Abstract

Proteins play a significant role in the colloidal stability and clarity of white wines [1]. However, under conditions of high temperatures during storage or transportation, the proteins themselves can self-aggregate into light-dispersing particles causing the so-called protein haze [2]. Formation of these unattractive precipitates in bottled wine is a common defect of commercial wines, making them unacceptable for sale [3]. Previous studies identified the presence of phenolic compounds in the natural precipitate of white wine [4], contributing to the hypothesis that these compounds could be involved in the mechanism of protein haze formation. The objective of this study was to isolate and identify the compounds contained in induced wine protein haze precipitate after alkaline hydrolysis. The heat-induced protein precipitate from five liters of white Moscatel of Alexandria wine was subjected to alkaline hydrolysis in 2 M NaOH, 10 mM EDTA and 1% (w/v) L-ascorbic acid following a protocol described elsewhere [5] with some modifications. The alkaline hydrolyzed sample was subjected to liquid-liquid extraction with ethyl acetate and evaporated to dryness. The extract was further fractionated using reversed phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD). The major compound present was found to be caffeic acid amongst other minor, unidentified compounds. Caffeic acid was identified by UV-vis spectra and the structure validated by 1H nuclear magnetic resonance (NMR). This work corroborates the observation that phenolic compounds, and caffeic acid in particular, may participate in wine protein haze formation since it is the major compound nonprotein compound present in Moscatel wine protein sediment.

References: [1] F.X. Sauvage, B. Bach, M. Moutounet, and A. Vernhet, Food Chemistry, 2010, 118, 26-34. [2] E.J. Waters, W. Wallace, and P.J. Williams, Journal of Agricultural and Food Chemistry, 1992, 40, 1514-1519. [3] G. Tabilo-Munizaga, T.A. Gordon, R. Villalobos-Carvajal, L. Moreno-Osorio, F.N. Salazar, M. Perez-Won, and S. Acuna, Food Chemistry , 2014, 155, 214-220. [4] M. Esteruelas, N. Kontoudakis, M. Gil, M.F. Fort, J.M. Canals, and F. Zamora, Food Research International, 2011, 44, 77-83. [5] Nardini, M., E. Cirillo, F. Natella, and C. Scaccini, Journal of Agricultural and Food Chemistry, 2002, 50, 5735-5741.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ricardo Chagas*, Ana Lourenço, Luísa Carvalho, Ricardo Ferreira, Sara Monteiro

*FCT/UNL

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

How do different oak treatment affect the sensory composition of Chenin blanc wines over time?

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

The role of tomato juice serum in malolactic fermentation in wine

Introduction: Malolactic fermentation (MLF) is a common process in winemaking to reduce wine acidity, maintain microbial stability and modify wine aroma. However, successful MLF is often hampered by their sluggish or stuck activity of malolactic bacteria (MLB) which may be caused by nutrient deficiency, especially when MLB are inoculated after alcoholic fermentation (Alexandre et al., 2004; Lerm et al., 2010). Identification and characterization of essential nutrients and growth factors for MLB allows for production of highly efficient nutrient supplements for MLF.

Micro-meteorological, compositional and transcriptional study of corvina grape color during ripening

Grape anthocyanin content and composition could affect the quality and the production strategies of red wines. Differences in the pigment composition modify the color properties in terms of hue, extractability and stability. Thus, for the production of a highly qualitative wine such as “Amarone”, variations in the pigment composition are not negligible. The aim of this work was the investigation of the anthocyanin profile changes during ripening in Corvina grapes, the main cultivar for the “Amarone” production. The experiment took place in 2015, in two vineyards located in Valpollicella (Italy).

Innovations in the use of bentonite in enology: interactions with grape and wine proteins, colloids, polyphenols and aroma compounds.

The use of bentonite in oenology rounds around the limpidity and the stability that determine consumer acceptability. As a matter of fact, the haze formation in wine reduces its commercial value and makes it unacceptable for sale. Stabilization treatments are, therefore, essential to ensure a long-time limpidity and to forecast the formation of deposits in the bottle. Bentonite that is normally used in oenology for clarifying-fining purpose, shows a natural clay-based mineral structure allowing it to swell and to jelly in water and hence in must and wine.

New biological tools to control and secure malolactic fermentation in high pH wines

Originally, the role of the malolactic fermentation (MLF) was simply to improve the microbial stability of wine via biological deacidification. However, there is an accumulation of evidence to support the fact that lactic acid bacteria (LAB) also contribute positively to the taste and aroma of wine. Many different LAB enter into grape juice and wine from the surface of grape berries, cluster stems, vine leaves, soil and winery equipment. Due to the highly selective environment of juices and wine, only a few types of LAB are able to grow.