Macrowine 2021
IVES 9 IVES Conference Series 9 Identification of caffeic acid as a major component of Moscatel wine protein sediment

Identification of caffeic acid as a major component of Moscatel wine protein sediment

Abstract

Proteins play a significant role in the colloidal stability and clarity of white wines [1]. However, under conditions of high temperatures during storage or transportation, the proteins themselves can self-aggregate into light-dispersing particles causing the so-called protein haze [2]. Formation of these unattractive precipitates in bottled wine is a common defect of commercial wines, making them unacceptable for sale [3]. Previous studies identified the presence of phenolic compounds in the natural precipitate of white wine [4], contributing to the hypothesis that these compounds could be involved in the mechanism of protein haze formation. The objective of this study was to isolate and identify the compounds contained in induced wine protein haze precipitate after alkaline hydrolysis. The heat-induced protein precipitate from five liters of white Moscatel of Alexandria wine was subjected to alkaline hydrolysis in 2 M NaOH, 10 mM EDTA and 1% (w/v) L-ascorbic acid following a protocol described elsewhere [5] with some modifications. The alkaline hydrolyzed sample was subjected to liquid-liquid extraction with ethyl acetate and evaporated to dryness. The extract was further fractionated using reversed phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD). The major compound present was found to be caffeic acid amongst other minor, unidentified compounds. Caffeic acid was identified by UV-vis spectra and the structure validated by 1H nuclear magnetic resonance (NMR). This work corroborates the observation that phenolic compounds, and caffeic acid in particular, may participate in wine protein haze formation since it is the major compound nonprotein compound present in Moscatel wine protein sediment.

References: [1] F.X. Sauvage, B. Bach, M. Moutounet, and A. Vernhet, Food Chemistry, 2010, 118, 26-34. [2] E.J. Waters, W. Wallace, and P.J. Williams, Journal of Agricultural and Food Chemistry, 1992, 40, 1514-1519. [3] G. Tabilo-Munizaga, T.A. Gordon, R. Villalobos-Carvajal, L. Moreno-Osorio, F.N. Salazar, M. Perez-Won, and S. Acuna, Food Chemistry , 2014, 155, 214-220. [4] M. Esteruelas, N. Kontoudakis, M. Gil, M.F. Fort, J.M. Canals, and F. Zamora, Food Research International, 2011, 44, 77-83. [5] Nardini, M., E. Cirillo, F. Natella, and C. Scaccini, Journal of Agricultural and Food Chemistry, 2002, 50, 5735-5741.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ricardo Chagas*, Ana Lourenço, Luísa Carvalho, Ricardo Ferreira, Sara Monteiro

*FCT/UNL

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

DNA and type of grain: which factor does better explain sensory differences of sessile and pedunculate oaks?

Sessile oak and pedunculate oak have shown several differences of interest for enological purposes. Tannic and aromatic composition among sessile oak or pedonculate oak has been well studied. Sessile oak is generally more aromatic than pedunculated, while the later is more tannic. This scientific point of view is rarely applied to classify oak in cooperages. Most coopers use the type of grain to distinguish wide and thin grain.

Metabolomic profile of red non-V. vinifera genotypes

Vitis vinifera L. is the most widely cultivated Vitis species which includes numerous cultivars. Owing to their superior quality of grapes, these cultivars were long considered the only suitable for the production of fine wines. However, the lack of resistance genes in V. vinifera against major grapevine pathogens, requires for its cultivation frequent spraying with large amount of fungicides. Thus, the search for alternative and more sustainable methods to control the grapevine pathogens have brought the breeders to focus their attention on other Vitis species. In fact, wild Vitis genotypes present multiple resistance traits against pathogens, such as powdery mildew, downy mildew and phylloxera.

To a better understanding of the impact of vine nitrogen status on volatile thiols from plot to transcriptome level

Volatile thiols contribute largely to the organoleptic characteristics and typicity of Sauvignon blanc wines. Among this family of odorous compounds, 3-sulfanylhexan-1-ol (3SH) and 4-methyl-4-sulfanylpentan-2-one (4MSP) have a major impact on wine flavor. These thiols are formed during alcoholic fermentation by the yeast from odorless and non-volatile precursors found in the berry and the must. The effect of vine nitrogen status on 3SH and 4MSP in Sauvignon blanc wine and on the glutathionylated and cysteinylated precursors of 3SH (Glut-3SH and Cys-3SH) was investigated in this study.

Comparison of various storage conditions to preserve polyphenols in red-grape pomace

Red grape pomace, a waste from wine production, can be valorised by extracting polyphenols, high-added value compounds used in cosmetics or oenology. For use at an industrial level, using green extraction techniques, pomace need to be stored before being processed. The aim of this study is to test various storage conditions in order to maintain high level of polyphenols over 180 days, while keeping storage cost economically interesting. In a first step, different storage conditions (ambient temperature or cooled (4°C) temperature, anaerobic (saturation with N2) or aerobic conditions, and addition of sulphur dioxide (SO2)) were compared on small samples (1 kg) packed in plastic pockets. The quality of storage was assessed by following the optical density of the pomace extract at 280 nm (DO 280 expressed as mg/l eq gallic acid), which is an indication of the amount of remaining extractable polyphenols.

Effect of malolactic fermentation in barrels or stainless steel tanks on wine composition. Influence of the barrel toasting

Ellagitannin, anthocyanin and woody volatile composition of Cabernet Sauvignon wines aged in oak barrels for 12 months was evaluated. Depending on the container where malolactic fermentation (MLF) was carried out, two wine modalities were investigated: wines with MLF carried out in stainless steel tanks and barrel-fermented wines. Three toasting methods (medium toast, MT; medium toast with watering, MTAA; noisette) were considered for ageing of each wine modality. Sensory analyses (triangle and rating tests) were also performed. Two-way ANOVA of the raw experimental data revealed that the toasting method and the container where MLF took place, as well as the interaction between both factors, have a significant influence (p < 0.05) on ellagitannin, anthocyanin and woody volatile profiles of Cabernet Sauvignon wines.