Macrowine 2021
IVES 9 IVES Conference Series 9 Identification of caffeic acid as a major component of Moscatel wine protein sediment

Identification of caffeic acid as a major component of Moscatel wine protein sediment

Abstract

Proteins play a significant role in the colloidal stability and clarity of white wines [1]. However, under conditions of high temperatures during storage or transportation, the proteins themselves can self-aggregate into light-dispersing particles causing the so-called protein haze [2]. Formation of these unattractive precipitates in bottled wine is a common defect of commercial wines, making them unacceptable for sale [3]. Previous studies identified the presence of phenolic compounds in the natural precipitate of white wine [4], contributing to the hypothesis that these compounds could be involved in the mechanism of protein haze formation. The objective of this study was to isolate and identify the compounds contained in induced wine protein haze precipitate after alkaline hydrolysis. The heat-induced protein precipitate from five liters of white Moscatel of Alexandria wine was subjected to alkaline hydrolysis in 2 M NaOH, 10 mM EDTA and 1% (w/v) L-ascorbic acid following a protocol described elsewhere [5] with some modifications. The alkaline hydrolyzed sample was subjected to liquid-liquid extraction with ethyl acetate and evaporated to dryness. The extract was further fractionated using reversed phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD). The major compound present was found to be caffeic acid amongst other minor, unidentified compounds. Caffeic acid was identified by UV-vis spectra and the structure validated by 1H nuclear magnetic resonance (NMR). This work corroborates the observation that phenolic compounds, and caffeic acid in particular, may participate in wine protein haze formation since it is the major compound nonprotein compound present in Moscatel wine protein sediment.

References: [1] F.X. Sauvage, B. Bach, M. Moutounet, and A. Vernhet, Food Chemistry, 2010, 118, 26-34. [2] E.J. Waters, W. Wallace, and P.J. Williams, Journal of Agricultural and Food Chemistry, 1992, 40, 1514-1519. [3] G. Tabilo-Munizaga, T.A. Gordon, R. Villalobos-Carvajal, L. Moreno-Osorio, F.N. Salazar, M. Perez-Won, and S. Acuna, Food Chemistry , 2014, 155, 214-220. [4] M. Esteruelas, N. Kontoudakis, M. Gil, M.F. Fort, J.M. Canals, and F. Zamora, Food Research International, 2011, 44, 77-83. [5] Nardini, M., E. Cirillo, F. Natella, and C. Scaccini, Journal of Agricultural and Food Chemistry, 2002, 50, 5735-5741.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Ricardo Chagas*, Ana Lourenço, Luísa Carvalho, Ricardo Ferreira, Sara Monteiro

*FCT/UNL

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

Anti/prooxidant activity of wine polyphenols in reactions of adrenaline auto-oxidation

Adrenaline (epinephrine) belongs to catecholamine class. It is a neurotransmitter and both a hormone which is released by the sympathetic nervous system and adrenal medulla in response to a range of stresses in order to regulate blood pressure, cardiac stimulation, relaxation of smooth muscles and other physiological processes. Adrenaline exhibits an effective antioxidant capacity (1). However, adrenalin is capable to auto-oxidation and in this case it generates toxic reactive oxygen intermediates and adrenochrome. Under in vitro conditions, auto-oxidation of adrenaline occurs in an alkaline medium (2).

HEAT BERRY : Sensitivity of berries ripening to higher temperature and impact on phenolic compounds in wine

The grapevine is an important economical crop that is very sensitive to climate changes and microclimate. The observations made during the last decades at a vineyard scale all concur to show the impact of climate change on vine physiology, resulting in accelerated phenology and earlier harvest (Jones and Davis 2000). It is well-known that berry content is affected by the ambient temperature. While the first experiences were primarily conducted on the impact of temperature on anthocyanin accumulation in the grape, few studies have focused on others component of phenolic metabolism, such as tannins.

Merging fast sensory profiling with non-targeted GC-MS analysis for multifactorial experimental wine making

Wine aroma is influenced by several viticultural and oenological factors. In this study we used experimental wine making in a full factorial design to determine the impact of grapevine age, must turbidity, and yeast strain on the aroma of Vitis vinifera L. cv. Riesling wines. A recently developed, non-targeted SPME-GC-MS fingerprinting approach for wine volatiles was used. This approach includes the segmentation and mathematical transformation of chromatograms in combination with Parallel Factor Analysis (PARAFAC) and subsequent deconvolution of important chromatogram segments.

The impact of different yeasts and harvest time on the wine quality of Beihong and Beimei (<I>V. vinifera x V. amurensis</I>)

Beihong and Beimei are two wine cultivars from ‘Muscat Hamberg’ (V. vinifera L.) and wild V. amurensis Rupr., which were released in China in 2008. Here,two enology practices were reported. Firstly, the impact of different yeasts including D254, GRE, K1, D21 and BDX on dry wine quality of Beihong and Beimei was investigated. For Beihong, among wines fermented by all yeasts, residual sugar content was the lowest, total anthocyanin and resveratrol contents were the highest in the wine by D254. However, the wine by D254 had lower titrable acid than those by the other yeasts except BDX.

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.