terclim by ICS banner
IVES 9 IVES Conference Series 9 Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Abstract

Variegation, a frequently observed trait in plants, is characterized by the occurrence of white or discoloured plant tissue. This phenomenon is attributed to genetic mosaicism or chimerism, potentially impacting the epidermal (L1) and subepidermal (L2) cell layers. In grapevine, variegation manifests as white or paler leaf, flower, or berry tissues, often leading to stunted growth and impeded development. Despite its prevalence, variegation in grapevines remains understudied. Notably, a natural mutant derived from Sultana, namely Bruce’s Sport, exhibits colour variegation in the leaves, although this occurrence only appears later in the growing season. Conversely, the flowers and berries are always variegated and are paler in colour. Furthermore, studies have observed that Bruce’s Sport displays a lower berry yield compared to the Sultana variety, along with reduced polyphenol oxidase (PPO) activity in the variegated tissues. This study aims to investigate the genetic basis of variegation in Bruce’s Sport and its effects on plant growth and development. To this extent, a transcriptomic analysis was employed comparing data obtained from flower tissue of Sultana and Bruce’s Sport. Additionally, differentially expressed genes were confirmed, aiding in the identification and characterization of genes associated with variegation in the Vitis genome, potentially uncovering candidates for future functional studies.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clara Holm1*, Nina Wiese1, Manuela Campa1, Johan Burger1, Justin Lashbrooke1

1 Genetics Department, Stellenbosch University, South Africa

Contact the author*

Keywords

variegation, grapevine, polyphenol oxidase activity, gene expression

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sensitivity of vis‐nir spectral indices to detect nitrogen deficiency and canopy function in cv. Barbera (Vitis vinifera L.) Grapevines

Precision nutrient management in viticulture can be addressed on the basis of a spatial characterization of within‐vineyard vine

Comparing vineyard irrigation management based in two different approaches: vegetation indices and SIMDualKc model

Water scarcity, high air temperatures, high vapor pressure deficit, and increasing frequency and intensity of extreme climatic events, namely heat waves, exert huge pressure on viticulture, as is the case of Mediterranean climates. Therefore, farmers rely more and more on irrigation to overcome these constraints. Deficit irrigation is a proved strategy to optimize irrigation efficiency and wine quality. The present study intends to demonstrate the application of precision techniques, namely remote sensing derived vegetation indices (VI) and an open source software, SIMDualKc, to compute crop evapotranspiration using the dual crop coefficient approach (Kcb + Ke), for deficit irrigation management.

Correlation between stable isotopic composition of the fungus aspergillus niger and its growth substrate and the extracted chitin

Wine is one of the most consumed and appreciated beverages in the world. Due to the growing attention paid to consumer health, there is a continuous search for sustainable alternatives to common additives (such as sulfur dioxide) used to preserve wine. An example is represented by chitosan, the main derivative of chitin, approved for the treatment of must and wine since 2009 by the “international organization of vine and wine” (OIV/OENO 338a/2009) and by the european commission (EC Reg. No. 606/2009).

USDA national grapevine germplasm resources: new curators, new directions

The National Plant Germplasm System (NPGS) in the United States Department of Agriculture safeguards numerous species. Grapevines are split in two locations: Davis, CA and Geneva, NY. The two germplasms maintain 43 Vitis species with over 4500 genetically unique accessions.

Grape texture characteristics are linked to one major qtl

Berry texture and berry skin mechanical properties have high agronomic importance, related to quality and marketing requirements of wine, table and raisin grapes.