terclim by ICS banner
IVES 9 IVES Conference Series 9 Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Abstract

Variegation, a frequently observed trait in plants, is characterized by the occurrence of white or discoloured plant tissue. This phenomenon is attributed to genetic mosaicism or chimerism, potentially impacting the epidermal (L1) and subepidermal (L2) cell layers. In grapevine, variegation manifests as white or paler leaf, flower, or berry tissues, often leading to stunted growth and impeded development. Despite its prevalence, variegation in grapevines remains understudied. Notably, a natural mutant derived from Sultana, namely Bruce’s Sport, exhibits colour variegation in the leaves, although this occurrence only appears later in the growing season. Conversely, the flowers and berries are always variegated and are paler in colour. Furthermore, studies have observed that Bruce’s Sport displays a lower berry yield compared to the Sultana variety, along with reduced polyphenol oxidase (PPO) activity in the variegated tissues. This study aims to investigate the genetic basis of variegation in Bruce’s Sport and its effects on plant growth and development. To this extent, a transcriptomic analysis was employed comparing data obtained from flower tissue of Sultana and Bruce’s Sport. Additionally, differentially expressed genes were confirmed, aiding in the identification and characterization of genes associated with variegation in the Vitis genome, potentially uncovering candidates for future functional studies.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clara Holm1*, Nina Wiese1, Manuela Campa1, Johan Burger1, Justin Lashbrooke1

1 Genetics Department, Stellenbosch University, South Africa

Contact the author*

Keywords

variegation, grapevine, polyphenol oxidase activity, gene expression

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Exploring aromatic profiles and environmental influences on berry chemistry of V. vinifera Riesling and Vitis sp. L’Acadie blanc in Quebec and Nova Scotia, Canada

Wine quality depends on grape biochemical constituents, including sugars, organic acids, amino acids, and bound and free aroma compounds, which are influenced by vineyard location and environmental factors such as temperature and precipitation [1].

From grapes to sparking wines: Aromas evaluation in a vine-spacing

Aim: Wine aromatic profile is a combination of viticulture and oenological practices and it is related to character, quality, and consumer acceptance. Based on the competition between soil capacity and canopy development, and on the potential to produce sparkling wines at Caldas, in the south region of Minas Gerais (Brazil) (21°55´S and 46°23´W, altitude 1.100m), the aim of this work was the evaluation of the development of aromas (secondary metabolites) from grapes to sparkling wines in a vine-spacing experiment and whether the distance between the vines can influence the aromatic profile of the sparkling wines (final product). 

A multidisciplinary approach to assess the impact of future drought scenarios on vineyard ecosystems

Drought events can strongly affect grapevine and berry physiology and subsequent wine quality, as widely demonstrated in controlled experiments.

Generation of radicals in wine by cavitation and study of their interaction with metals, phenols and carboxylic acids

High-power ultrasounds have been related to an accelerated aging of wines, an effect that has been associated to the formation of radical species caused by the cavitation phenomenon [1]. This phenomenon consists of the formation of bubbles in the liquid medium that, when they collapse, cause high-pressure hot spots and temperatures of up to 4800 k [2], notably increasing the reactivity in the medium.

Egg allergens in wine. Validation of a new automated method for ovalbumin quantification

Ovalbumin (ova), a natural clarifying protein, is particularly suitable for clarifying red wines. It helps improve the tannic and polyphenolic stability of the wine by removing the most astringent tannins and contributing to soften and refine the structure. Ova binds to suspended particles, proteins, polysaccharides, and, to a lesser extent, tannins through electrostatic and hydrophobic interactions, forming large complexes that can be removed from the wine through fining and/or filtration before bottling.