terclim by ICS banner
IVES 9 IVES Conference Series 9 Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Abstract

Variegation, a frequently observed trait in plants, is characterized by the occurrence of white or discoloured plant tissue. This phenomenon is attributed to genetic mosaicism or chimerism, potentially impacting the epidermal (L1) and subepidermal (L2) cell layers. In grapevine, variegation manifests as white or paler leaf, flower, or berry tissues, often leading to stunted growth and impeded development. Despite its prevalence, variegation in grapevines remains understudied. Notably, a natural mutant derived from Sultana, namely Bruce’s Sport, exhibits colour variegation in the leaves, although this occurrence only appears later in the growing season. Conversely, the flowers and berries are always variegated and are paler in colour. Furthermore, studies have observed that Bruce’s Sport displays a lower berry yield compared to the Sultana variety, along with reduced polyphenol oxidase (PPO) activity in the variegated tissues. This study aims to investigate the genetic basis of variegation in Bruce’s Sport and its effects on plant growth and development. To this extent, a transcriptomic analysis was employed comparing data obtained from flower tissue of Sultana and Bruce’s Sport. Additionally, differentially expressed genes were confirmed, aiding in the identification and characterization of genes associated with variegation in the Vitis genome, potentially uncovering candidates for future functional studies.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clara Holm1*, Nina Wiese1, Manuela Campa1, Johan Burger1, Justin Lashbrooke1

1 Genetics Department, Stellenbosch University, South Africa

Contact the author*

Keywords

variegation, grapevine, polyphenol oxidase activity, gene expression

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The state-of-the-art of grapevine biotechnology and new breeding technologies (NBTS)

 The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies.

Reduced bunch compactness in a clone of Tempranillo associates with a complex reciprocal translocation detected by long-read sequencing genomics

Grapevine cultivars are vegetatively propagated to maintain their varietal attributes. However, spontaneous somatic variation emerges during prolonged periods of vegetative growth, providing an opportunity for the natural improvement of traditional grapevine cultivars. Notably, reduction in bunch compactness is a favorable trait in viticulture, offering advantages such as decreased susceptibility to bunch fungal diseases, and a more uniform ripening of berries. To unravel the genetic and developmental mechanisms behind bunch compactness variation, we examined a somatic variant of Tempranillo Tinto cultivar with loose bunches. We found that the mutant clone exhibits a ~50% reduction in pollen viability compared to typical Tempranillo clones.

Use of fumaric acid on must or during alcoholic fermentation

Fumaric acid has been approved by the OIV in 2021 for its application on wine to control the growth and activity of lactic acid bacteria. Fumaric acid is currently being evaluated by the OIV as an acidifier of must and wine. Investigations during the 2023 vintage provided further information on its use on must or during AF, thus completing information provided during the previous vintage.

Water and nutritional savings shape non-structural carbohydrates in grapevine (Vitis vinifera L.) cuttings

Global changes and sustainability challenge researchers in saving water and nutrients. The response of woody crops, which can be forced at facing more drought events during their life, is particularly important. Vitis vinifera can be an important model for its relevance in countries subjected to climate changes and its breeding, requiring cuttings plantation and strong pruning.

Biochemical responses of crimson seedless (Vitis vinifera) grapevines to altered micro climatic conditions and different water treatments in the Breede River Valley of South Africa

The South African Table grape industry has to expand to new markets with high quality niche products, but limited water availability threatens sustainable production. To overcome this challenge and to obtain high-quality products for the new markets, require constant technological advancement. Currently, limited available scientific information about growth balances and physiology and especially grape quality parameters, hinders technological advancement and thus efficient regulatory management of the morphological, chemical, and pathological status of table grapes, especially in response to abiotic factors.