terclim by ICS banner
IVES 9 IVES Conference Series 9 Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Molecular characterization of a variegated grapevine mutant cv Bruce’s Sport

Abstract

Variegation, a frequently observed trait in plants, is characterized by the occurrence of white or discoloured plant tissue. This phenomenon is attributed to genetic mosaicism or chimerism, potentially impacting the epidermal (L1) and subepidermal (L2) cell layers. In grapevine, variegation manifests as white or paler leaf, flower, or berry tissues, often leading to stunted growth and impeded development. Despite its prevalence, variegation in grapevines remains understudied. Notably, a natural mutant derived from Sultana, namely Bruce’s Sport, exhibits colour variegation in the leaves, although this occurrence only appears later in the growing season. Conversely, the flowers and berries are always variegated and are paler in colour. Furthermore, studies have observed that Bruce’s Sport displays a lower berry yield compared to the Sultana variety, along with reduced polyphenol oxidase (PPO) activity in the variegated tissues. This study aims to investigate the genetic basis of variegation in Bruce’s Sport and its effects on plant growth and development. To this extent, a transcriptomic analysis was employed comparing data obtained from flower tissue of Sultana and Bruce’s Sport. Additionally, differentially expressed genes were confirmed, aiding in the identification and characterization of genes associated with variegation in the Vitis genome, potentially uncovering candidates for future functional studies.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Clara Holm1*, Nina Wiese1, Manuela Campa1, Johan Burger1, Justin Lashbrooke1

1 Genetics Department, Stellenbosch University, South Africa

Contact the author*

Keywords

variegation, grapevine, polyphenol oxidase activity, gene expression

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Guard cell metabolism – A key for regulating drought resilience?

In view of increasing drought frequencies due to climate change, enhancing grapevine resilience to water scarcity has become vital for sustainable viticulture.

Spectral characterisation of fungal diseases on Vitis vinifera leaves

Aims: The aims of this study were to (1) detect alterations in the reflectance spectra of vines with fungal diseases, (2) map these alterations, and (3) determine the best wavelengths which may be used as early indicators of fungal diseases in vines.

Identification, quantification and organoleptic impact of « dried fruit » molecular markers in Merlot and Cabernet Sauvignon grapes and in red wines

The aromas found in young Bordeaux red wines made with Merlot and Cabernet Sauvignon suggest a complex mixture of aromas of fresh red fruits such as cherry or blackberry for Merlot, and strawberry or blackcurrant for Cabernet Sauvignon. The aromas of these wines are closely linked with the maturity of the grapes. The climate change that has occurred during the last decade in Bordeaux has induced changes in the ripening conditions of grape berries. It is now widely admitted that over-ripening of the berries during hot and dry summers results in the development of characteristic flavors reminiscent of cooked fruits (fig, prune). The presence of these overriding odors found in both musts and young wines affects the quality and subtlety of the wine flavor and may shorten its shelf life.

Oenological compatibility of biocontrol yeasts applied to wine grapes 

Antagonistic yeasts applied to wine grapes must be compatible with the thereafter winemaking process, avoiding competition with the fermentative Saccharomyces cerevisiae or affecting wine flavour. Therefore, fifteen epiphytic yeasts (6 Metschnikowia sp., 6 Hanseniaspora uvarum, 3 Starmerella bacillaris) previously selected for its biocontrol ability against Alternaria on wine grapes were evaluate for possible competition with S. cerevisiae by the Niche Overlap Index (NOI) employing YNB agar media with 10 mM of 17 different carbonate sources present in wine grapes (proline, asparagine, alanine, glutamic acid, tirosine, arginine, lisine, methionine, glicine, malic acid, tartaric acid, fructose, melibiose, raffinose, rhamnose, sucrose, glucose).

Impacts of environmental variability and viticultural practices on grapevine behaviour at terroir scales

Climate change poses several challenges for the wine-industry in the 21st century. Adaptation of viticultural and winemaking practices are therefore essential to preserve wine quality and typicity. Given the complex interactions between physical, biological and human factors at terroir scales, studies conducted at these fine scales allow to better define the local environment and its influences on grapevine growth and berry ripening.