terclim by ICS banner
IVES 9 IVES Conference Series 9 Towards a better understanding of the root system diversity and plasticityin young grafted vines using 2D imaging and 3D modelling tools

Towards a better understanding of the root system diversity and plasticityin young grafted vines using 2D imaging and 3D modelling tools

Abstract

Three-dimensional functional-structural root architecture models, which decompose the root system architecture (RSA) into elementary developmental processes such as root emission, axial growth, branching patterns and tropism have become useful tools for (i) reconstructing in silico the spatial and temporal dynamics of root systems in a soil volume, (ii) analyzing their genotypic diversity and plasticity to the environment, and (iii) overcoming the bottleneck associated with their visualization and measurement in situ. Here, we present an original work on RSA phenotyping and modelling in grapevine. First, we developed 2D image-based analysis pipelines to quantify morphological and architectural traits in young grafts. Second, we parametrized and validated the 3D root model Archisimple on two rootstock genotypes (RGM, 1103P) grafted with V. vinifera Cabernet-Sauvignon and grown in different controlled conditions (rhizotrons, pots, tubes). Finally, we experimentally studied the sensitivity of RSA to initial carbon availability using hardwood cuttings of different lengths (8, 20, 30 and 50 cm). Results showed that the number of adventitious roots and their emergence rate were affected by changes in cutting length, whereas RSA traits related to elongation and branching remained relatively stable. Simulated root systems differed between rootstocks, with RGM having more shallow roots and less deep root length density than 1103P. This study highlighted how a modelling approach provides a deeper understanding of the genetic, environmental, and endogenous factors that determine rooting ability and early development of RSA. This knowledge can be applied in nurseries, for example, to improve the successful establishment of grafted vines after planting.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Mathieu Larrey1*, Jean-Pascal Tandonnet1, Clément Saint Cast1, Philippe Vivin1

1 EGFV, University of Bordeaux, Bordeaux Sciences Agro, INRAE, ISVV, 33882 Villenave d’Ornon, France

Contact the author*

Keywords

root system architecture, functional-structural root architecture model, root phenotyping, rootstock

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Sensory and consumer perceptions, and consumption barriers of low and no-alcohol wines in Trentino/Alto Adige

The growing demand for non-alcoholic beverages, driven by health-conscious consumers and shifting social norms, has positioned dealcoholized wines as a promising alternative in the global beverage industry (Akhtar et al., 2025, in press; Kakroo, 2024).

Novel ATR-FTIR and UV-Vis spectral markers for assessing the Prooxidant/Antioxidant Balance (PAB) in white wines

The browning index (BI), based on the absorbance at 420 nm, is a common oxidation marker in white wines, typically measured after thermal stress (50–60 °C for 5 up to 12 days) in air-saturated wines.

MOUSY OFF-FLAVOURS IN WINES: UNVEILING THE MICROORGANISMS BEHIND IT

Taints and off-flavours are one of the major concerns in the wine industry and even if the issues provoked by them are harmless, they can still have a negative impact on the quality or on the visual perception of the consumer. Nowadays, the frequency of occurrence of mousy off-flavours in wines has increased.
The reasons behind this could be the significant decrease in sulphur dioxide addition during processing, the increase in pH or even the trend for spontaneous fermentation in wine. This off-flavour is associated with Brettanomyces bruxellensis or some lactic acid bacteria metabolisms.

Egg allergens in wine. Validation of a new automated method for ovalbumin quantification

Ovalbumin (ova), a natural clarifying protein, is particularly suitable for clarifying red wines. It helps improve the tannic and polyphenolic stability of the wine by removing the most astringent tannins and contributing to soften and refine the structure. Ova binds to suspended particles, proteins, polysaccharides, and, to a lesser extent, tannins through electrostatic and hydrophobic interactions, forming large complexes that can be removed from the wine through fining and/or filtration before bottling.

Frost risk projections in a changing climate are highly sensitive in time and space to frost modelling approaches

Late spring frost is a major challenge for various winegrowing regions across the world, its occurrence often leading to important yield losses and/or plant failure. Despite a significant increase in minimum temperatures worldwide, the spatial and temporal evolution of spring frost risk under a warmer climate remains largely uncertain. Recent projections of spring frost risk for viticulture in Europe throughout the 21st century show that its evolution strongly depends on the model approach used to simulate budburst. Furthermore, the frost damage modelling methods used in these projections are usually not assessed through comparison to field observations and/or frost damage reports.
The present study aims at comparing frost risk projections simulated using six spring frost models based on two approaches: a) models considering a fixed damage threshold after the predicted budburst date (e.g BRIN, Smoothed-Utah, Growing Degree Days, Fenovitis) and b) models considering a dynamic frost sensitivity threshold based on the predicted grapevine winter/spring dehardening process (e.g. Ferguson model). The capability of each model to simulate an actual frost event for the Vitis vinifera cv. Chadonnay B was previously assessed by comparing simulated cold thermal stress to reports of events with frost damage in Chablis, the northernmost winegrowing region of Burgundy. Models exhibited scores of κ > 0.65 when reproducing the frost/non-frost damage years and an accuracy ranging from 0.82 to 0.90.
Spring frost risk projections throughout the 21st century were performed for all winegrowing subregions of Bourgogne-Franche-Comté under two CMIP5 concentration pathways (4.5 and 8.5) using statistically downscaled 8×8 km daily air temperature and humidity of 13 climate models. Contrasting results with region-specific spring frost risk trends were observed. Three out of five models show a decrease in the frequency of frost years across the whole study area while the other two show an increase that is more or less pronounced depending on winegrowing subregion. Our findings indicate that the lack of accuracy in grapevine budburst and dehardening models makes climate projections of spring frost risk highly uncertain for grapevine cultivation regions.