terclim by ICS banner
IVES 9 IVES Conference Series 9 Reduced berry skin epi-cuticular wax and cutin accumulation associates with a genomic deletion and increased polyphenols extractability in a clone of Tempranillo Tinto 

Reduced berry skin epi-cuticular wax and cutin accumulation associates with a genomic deletion and increased polyphenols extractability in a clone of Tempranillo Tinto 

Abstract

Tempranillo Tinto (TT) is the third-most planted red wine variety in the world, and it is mostly grown in the Iberian Peninsula. Spontaneous somatic variation appearing during vegetative propagation can be exploited to improve elite varieties as Tempranillo Tinto, including the selection of new phenotypes enhancing berry quality. We described previously that a somatic variant of TT with darker fruit color, the clone VN21, exhibits increased extractability of polyphenols during the winemaking process. To unravel the molecular mechanism underlying this phenomenon, we performed whole-genome resequencing to compare VN21 to other TT clones, revealing a 10 Mb deletion in chromosome 11 that likely affected only the L1 meristem cell layer of VN21 and tissues derived from it, such as external cell layers of berry skin. A putative loss-of-function allele of an ABCG32 gene (homologous to cuticle biogenesis transporters), was left hemizygous in this segment after the deletion in VN21. Scanning electron microscopy images suggested a lower content epi-cuticular wax in the berry cuticle of VN21, which likely leads to the shiny colour of VN21 berries. A GC-MS analysis of epi-cuticular waxes and cutins extracted from berry skin and leaves confirmed a general decrease in the accumulation of cuticle constituent compounds in VN21, supporting a role for the mutated ABCG32 transporter in the phenotype. Our findings show that somatic mutations altering berry cuticle biogenesis can have an effect on the extractability of polyphenols from the berry skin, which could be exploited for varietal wine innovation.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Carolina Royo1*, Yolanda Ferradás1,2, Robin Bosman 3, Fernando Alba-Elías 4, Javier Ibáñez 1, Justin Lashbrooke 3, José Miguel Martínez-Zapater 1, Pablo Carbonell-Bejerano 1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño. Spain
2 Current address: Facultad de Biología, Universidad de Santiago de Compostela, 15872 Santiago de Compostela. Spain
3 South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, South Africa
4 Universidad de La Rioja, Departamento de Ingeniería Mecánica, Logroño, Spain

Contact the author*

Keywords

somatic variation, whole genome resequencing, deletion, waxes, GC-MS

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Evaluation of Furmint clones in the Tokaj Wine Region

The ’Furmint’ is the most important grape variety in the Tokaj Wine Region, constituting around 65% of its vineyard area. Before the phylloxera disease many types were grown, but as selection started in the 20th century, its diversity dramatically narrowed. As a result, the cultivation of Furmint was based mainly on two heavy-cropping clones, T.85 and T.92 at the end of the ’80s. Aims of present clone research take into account that after solely quantity as target, quality emerged in the 1990’s and most recently, typicity appeared as more private estates began their own selection program.

Effect of foliar application of Ca, Si and their combination on grape volatile composition

Calcium (Ca) is an important nutrient for plants which plays key signaling and structural roles. It has been observed that exogenous Ca application favors the pectin accumulation and inhibition of polygalacturonase enzymes, minimizing fruit spoilage. Silicon (Si) is a non-essential element which has been found to be beneficial for improving crop yield and quality, as well as plant tolerance to diverse abiotic and biotic stress factors. The effect of Si supply to grapevine has been assessed in few investigations, which reported positive changes in grape quality and must composition.

Raffinose: a sweet solution for grapevine drought tolerance

Water tolerance in plants is often associated with the accumulation of osmotic protectants, which are secondary metabolites that can help the plant to cope with water stress. One of the key osmotic protectants is a sugar called Raffinose, which is synthesized by a family of enzymes called Raffinose synthases. In this work, we focused on one of these enzymes, VviRAF2, which is a gene that shows different expression levels and genetic variants (SNPs) among different grapevine cultivars, ranging from tolerant to susceptible to water stress, and the transcription factors that may regulate the expression of this gene family.

Moderated consumption of alcoholic beverages and cancer risk

One on three cases of cancer is associated with lifestyle and nutritional patterns, and the excessive intake of alcoholic beverages is a well established risk factor. Moderate drinking has been associated with reduced or increased risk of various types of cancer, but the clinical relevance of the risk rates has not been evaluated in ad hoc prospective investigations.

On the losses of dissolved CO2 during champagne aging

A misconception lingers in the minds of some wine consumers that Champagne wines don’t age. It’s largely a myth, certainly as far as the best cuvees are concerned. Actually, during the so-called autolysis period of time (in the closed bottle, after the “prise de mousse”), complex chemical reactions take place when the wine remains in contact with the dead yeast cells, which progressively bring complex and very much sought-after aromas to champagne. Nevertheless, despite their remarkable impermeability to liquid and air, caps or natural cork stoppers used to cork the bottles are not 100% hermetic with regard to gas transfers. Gas species therefore very slowly diffuse through the cap or cork stopper, along their respective inverse partial pressure. After the “prise de mousse”, because the partial pressure of CO2 in the bottleneck reaches up to 6 bars (at 12 °C), gaseous CO2 progressively diffuse from the bottle to the ambient air
(where the partial pressure of gaseous CO2 is only of order of 0,0004 bar).