terclim by ICS banner
IVES 9 IVES Conference Series 9 Reduced berry skin epi-cuticular wax and cutin accumulation associates with a genomic deletion and increased polyphenols extractability in a clone of Tempranillo Tinto 

Reduced berry skin epi-cuticular wax and cutin accumulation associates with a genomic deletion and increased polyphenols extractability in a clone of Tempranillo Tinto 

Abstract

Tempranillo Tinto (TT) is the third-most planted red wine variety in the world, and it is mostly grown in the Iberian Peninsula. Spontaneous somatic variation appearing during vegetative propagation can be exploited to improve elite varieties as Tempranillo Tinto, including the selection of new phenotypes enhancing berry quality. We described previously that a somatic variant of TT with darker fruit color, the clone VN21, exhibits increased extractability of polyphenols during the winemaking process. To unravel the molecular mechanism underlying this phenomenon, we performed whole-genome resequencing to compare VN21 to other TT clones, revealing a 10 Mb deletion in chromosome 11 that likely affected only the L1 meristem cell layer of VN21 and tissues derived from it, such as external cell layers of berry skin. A putative loss-of-function allele of an ABCG32 gene (homologous to cuticle biogenesis transporters), was left hemizygous in this segment after the deletion in VN21. Scanning electron microscopy images suggested a lower content epi-cuticular wax in the berry cuticle of VN21, which likely leads to the shiny colour of VN21 berries. A GC-MS analysis of epi-cuticular waxes and cutins extracted from berry skin and leaves confirmed a general decrease in the accumulation of cuticle constituent compounds in VN21, supporting a role for the mutated ABCG32 transporter in the phenotype. Our findings show that somatic mutations altering berry cuticle biogenesis can have an effect on the extractability of polyphenols from the berry skin, which could be exploited for varietal wine innovation.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Carolina Royo1*, Yolanda Ferradás1,2, Robin Bosman 3, Fernando Alba-Elías 4, Javier Ibáñez 1, Justin Lashbrooke 3, José Miguel Martínez-Zapater 1, Pablo Carbonell-Bejerano 1

1 Instituto de Ciencias de la Vid y del Vino, Finca La Grajera, Ctra. de Burgos Km. 6, 26007 Logroño. Spain
2 Current address: Facultad de Biología, Universidad de Santiago de Compostela, 15872 Santiago de Compostela. Spain
3 South African Grape and Wine Research Institute, Stellenbosch University, Stellenbosch, South Africa
4 Universidad de La Rioja, Departamento de Ingeniería Mecánica, Logroño, Spain

Contact the author*

Keywords

somatic variation, whole genome resequencing, deletion, waxes, GC-MS

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.

Model comparison and parametrization strategies for accurate RGB‑Based grapevine phenology classification

Accurate monitoring of grapevine phenology is essential for vineyard management and for understanding seasonal responses to climate variability (Reis et al., 2020).

Practical Aspects of Viticultural Zoning In South Africa

Depuis 1973, une commission statutaire administre la législation qui régit le zonage vitivinicole en Afrique du Sud. La province «Le Cap de l’ouest» cerne toutes les zones viticoles sauf quatre unités. Pour la plupart, le Cap de l’ouest a un climat méditerranéen. Les zones viticoles – qui produisent les «vins d’origine» – sont des régions, des districts, des quartiers et des domaines. Les régions sont vastes, séparées par la topographie, par ex. des chaînes de montagnes et des fleuves. Généralement, chaque région représente une zone climatique. Le climat de chaque district est plus homogène. Les quartiers sont exactement délimités par le climat, la topographie et la géologie. Les domaines sont les plus petits. Chaque domaine doit avoir un seul propriétaire.

Identification of loci associated with specialised metabolites in Vitis vinifera

Secondary (or specialised) metabolites such as terpenes and phenolic compounds are produced by plants for various roles which include defence against pathogens and herbivores, protection against abiotic stress, and plant signalling. Additionally, these metabolites influence grapevine quality traits such as colour, aroma, taste, and nutritional value. However, the biosynthesis of these metabolites is often complex and controlled by multiple genes which in grapevine are predominantly uncharacterised.

Wine metabolomics and sensory profile in relation to terroir: A case study focusing on different wine-growing areas of Piacenza Province (Italy)

Aim: In this work, we have optimized a robust methodology for investigating possible correlations between the phytochemical profile of wine and the terroir (including the climate), considering the specific wine-growing area. In particular, the untargeted metabolomic and sensorial profiles of Gutturnio DOC commercial wines (both still and “frizzante” types) from different production areas in the Piacenza province were determined. The geographical areas taken into consideration for this study consisted in Val Tidone, Val Nure and Val d’Arda.