Application of nitrogen forms such as nitrate, urea, and amino acids effects on leaf and berry physiology and wine quality

Fungus-resistant grape varieties (frgv) are an important field of research in viticulture, as they represent a way of reducing the use of copper-containing pesticides and thus minimising the environmental impact. The literature suggests that resistant grape varieties are a promising solution to the problem of using copper-containing pesticides in viticulture and that their quality has improved in recent years. However, there are still challenges in the acceptance and dissemination of FRGV by wine producers and consumers.

Somatic embryogenesis (SE) has been used in a variety of biotechnology applications such as virus elimination, cryopreservation, induced mutagenesis and genetic transformation. The SE induction process may cause DNA alterations and ploidy changes, which may provide a source of genetic variability useful for the improvement of agronomic characteristics of plants. This research aims at investigating the spontaneous alterations of the genome in grapevine plants regenerated through SE. Regenerants obtained from different embryogenic events from three different grapevine genotypes (Catarratto, Frappato and Nero d’Avola) were analysed.

To our knowledge all the studies about laccase kinetics and its inhibition have been performed with substrates and conditions very different from those of real grape juice. Moreover, none of these researches really measure enzymatic browning, since they have not taken into account what happens after the oxidation of o-diphenols in o-diquinones and their subsequent polymerization to form melanins1. For that reason, the aim of this research was to develop a new model to measure the kinetics of browning caused by Botrytis cinerea laccase under conditions much closer to those of grape juice and using the substrates naturally present in it.

Background: hydroxycinnamic acids (HCA), present in pulp and skin of grapes, are relevant compounds in red winemaking

 The manipulation of the genetic basis controlling grapevine adaptation and phenotypic plasticity can be performed either by classical genetics or biotechnologies.