Unravel the underlying mechanisms of delaying ripening techniques in grapevine

Estimation of vineyard leaf area index (LAI) is an important aspect for the winegrowers. However, tracking and monitoring are difficult tasks due to time constraints. Satellite and unmanned aerial vehicle (UAV) imaging have become a practical monitoring method for LAI. Nevertheless, for a proper LAI determination, the image’s spatial resolution is a key factor, since low-resolution images are incapable of distinguishing between adjacent vines due to the large area covered in each pixel, this leads to misinterpretation or generalisation of vineyard information.

Grapevine is a crop of great economic importance for several countries. The intensification of grapevine production has mostly been sustained by the increasing use of water resources at the expense of the environmental water balance. Moreover, in the last decades, climate change and the consequent expansion of drought have further compromised water availability, making current agricultural systems even more fragile both ecologically and economically. Recently, many research groups have highlighted the important role of endophytes in facilitating plant growth under optimal or stressful conditions. Within the framework of the PRIMA project, we aim to investigate the possible exploitation of the natural endophyte biodiversity as a sustainable tool to make grapevine plants more resilient to water deficit environmental conditions.

To characterize a wine, the most frequently used criteria describe its color, its origin, the grape varieties from which they come, or even for white wines its residual sugar content (dry, semi-dry, sweet). In france, the system of appellations of origin set up in 1919 was initially based solely on the notoriety and origin of the wines. But given the unfavorable consequences that this lack of details generated, the public authorities quickly integrated in 1927 into the “capus” law criteria for access to designations of origin, relating to the specific characteristics of the soils of the vineyards and the grape varieties used, in particular exclusion of interspecific hybrid varieties. In 1935 the creation of the aoc system confirmed the interest in precisely defining all the production conditions that must be implemented to be able to claim the benefit of an aoc, and grape varieties were an essential condition for acquisition.

Grapevine (Vitis vinifera L.) is a challenging plant species to transform and regenerate due to its complex genome and biological characteristics. This limits the development of cisgenic and gene-edited varieties. One hurdle is selecting the best starting tissue for the transformation process, much like isolating suitable tissue for protoplasts. One promising method involves delivering CRISPR/Cas components to protoplasts isolated from embryogenic calli, which are then induced to regenerate. However, this process is inefficient, time-consuming, and only applicable to a few genotypes. To enhance grapevine regeneration efficiency, the expression of developmental and plant growth regulators shows promise in escaping the recalcitrance encountered in traditional tissue culture methods.

La recherche, conduite en 2002, a envisagé l’estimation, a priori, de l’effet du changement mesoclimatique sur le potentiel qualitatif de la région viticole de la Serra Gaúcha (Vallée du Rio das Antas) – Brésil, en fonction de la construction de 3 usines hydroélectriques.