terclim by ICS banner
IVES 9 IVES Conference Series 9 Effects of stress memory on grapevine resilience in response to recurrent drought and recovery events 

Effects of stress memory on grapevine resilience in response to recurrent drought and recovery events 

Abstract

Plants have evolved different strategies to cope with environmental stresses and, although still debated, it was observed that they can remember past stress occurrence.

Anatomical and physiological adjustments have been observed in different grapevine cultivars after repeated drought exposure, however epigenetic, transcriptional and biochemical changes associated with drought-primed ecological memory have been poorly studied.

This work was conceived to test whether exposure to recurring events of mild drought could prime vines to endure severe drought stress. Particularly, we investigated whether the expected improved stress tolerance of Vitis vinifera cv Nebbiolo plants subjected over years to moderate and long-lasting water stress events (WS-primed) depended on molecular memory phenomena or on resetting of stress-induced signals. To this aim, a combined multidisciplinary approach, involving eco-physiological, anatomical, biochemical and molecular analyses was adopted. First results revealed that WS-primed vines had reduced gas exchange in well-watered conditions, but at the end of WS imposition were able to maintain higher transpiration and assimilation rates with respect to unprimed plants. Moreover, WS-primed plants accumulated lower amounts of root abscisic acid and had higher content of resveratrol and viniferin, suggesting an increased antioxidant capacity that could help them in counteracting stress effects at the cellular level. WGBS analysis is ongoing to profile changes in DNA methylation landscapes in search of epigenetic signatures associated with specific transcriptome and physiological modifications.

In a future perspective, the gained information will deliver a predictive framework to estimate the impact of moderately dry periods on vine performance, considering memory-associated protective effects against drought.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Amedeo Moine1, Paolo Boccacci1, Walter Chitarra1,2, Luca Nerva1,2, Giorgio Gambino1, Irene Perrone1, Chiara Pagliarani1*

1 Institute for Sustainable Plant Protection, National Research Council (IPSP-CNR), Strada delle Cacce 73, 10135 Torino (Italy)
2 Research Centre for Viticulture and Enology, Council for Agricultural Research and Economics (CREA-VE), Via XXVIII Aprile 26, 31015 Conegliano (Italy)

Contact the author*

Keywords

eco-physiology, recurring drought, priming, transcriptome reprogramming, epigenetic signature

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The impact of different yeasts and harvest time on the wine quality of Beihong and Beimei (<I>V. vinifera x V. amurensis</I>)

Beihong and Beimei are two wine cultivars from ‘Muscat Hamberg’ (V. vinifera L.) and wild V. amurensis Rupr., which were released in China in 2008. Here,two enology practices were reported. Firstly, the impact of different yeasts including D254, GRE, K1, D21 and BDX on dry wine quality of Beihong and Beimei was investigated. For Beihong, among wines fermented by all yeasts, residual sugar content was the lowest, total anthocyanin and resveratrol contents were the highest in the wine by D254. However, the wine by D254 had lower titrable acid than those by the other yeasts except BDX.

Soil and topography effects on water status and must composition of chardonnay in burgundy & a mini meta‐analysis of the δ 13C/water potentials correlation

The measurement of carbon isotopic discrimination in grape sugars 13 at harvest (δ C) is an integrated assessment of water status during ripening.

Genotypic variability in root architectural traits and putative implications for water uptake in grafted grapevine

Root system architecture (RSA) is important for soil exploration and edaphic resources acquisition by the plant, and thus contributes largely to its productivity and adaptation to environmental stresses, particularly soil water deficit. In grafted grapevine, while the degree of drought tolerance induced by the rootstock has been well documented in the vineyard, information about the underlying physiological processes, particularly at the root level, is scarce, due to the inherent difficulties in observing large root systems in situ. The objectives of this study were to determine genetic differences in the root architectural traits and their relationships to water uptake in two Vitis rootstocks genotypes (RGM, 140Ru) differing in their adaptation to drought. Young rootstocks grafted upon the Riesling variety were transplanted into cylindrical tubes and in 2D rhizotrons under two conditions, well watered and moderate water stress. Root traits were analyzed by digital imaging and the amount of transpired water was measured gravimetrically twice a week. Root phenotyping after 30 days reveal substantial variation in RSA traits between genotypes despite similar total root mass; the drought-tolerant 140Ru showed higher root length density in the deep layer, while the drought-sensitive RGM was characterised by shallow-angled root system development with more basal roots and a larger proportion of fine roots in the upper half of the tube. Water deficit affected canopy size and shoot mass to a greater extent than root development and architectural-related traits for both 140Ru and RGM, suggesting vertical distribution of roots was controlled by genotype rather than plasticity to soil water regime. The deeper root system of 140Ru as compared to RGM correlated with greater daily water uptake and sustained stomata opening under water-limited conditions but had little effect on above-ground growth. Our results highlight that grapevine rootstocks have constitutively distinct RSA phenotypes and that, in the context of climate change, those that develop an extensive root network at depth may provide a desirable advantage to the plant in coping with reduced water resources.

Methyl jasmonate versus nano methyl jasmonate: effects on anthocyanins mature evolution in monastrell grapes

The climate change is afecting particulary to the South of Spain, with high temperatures. It is important to develop new strategies in order to mantain the quality of wines

Méthodologie pour application et valorisation des études de terroir dans les caves cooperatives des Côtes du Rhône (France)

L’appellation d’origine contrôlée “Côtes du Rhône” se caractérise par une très forte implantation du mouvement coopératif. Afin de mieux exploiter le potentiel qualitatif de leurs terroirs, plusieurs coopératives élaborent des “cuvées terroir”, résultat des sélections de vendanges provenant de différents secteurs.