Phenological stage dependency of Cabernet Sauvignon and Grenache response to water and nutrient limitation 

Plasmopara viticola, the causal agent of downy mildew (DM), and Uncinula necator, the causal agent of powdery mildew (PM), are two of the main phytopathogenic microorganisms causing major economic losses in the primary sector, especially in the wine sector, by wilting bunches and leaves with a consequent decrease in the photosynthetic rate of the plant and in the annual yield. Currently, the most widespread methods for planning spraying are based on the 3-10 rule, which states that the first application should take place when: (i) the air temperature is greater than 10°C; (ii) shoots are equal or greater than 10 cm; and (iii) a minimum of 10 mm rainfall within 24–48 hours has occurred, or at the beginning of the bud break with periodic applications according to the manufacturer’s instructions.

Aim: The regulation of the vegetative-reproductive balance of a vineyard is a critical aspect for the quality of grapes. Early leaf removal, generally applied before the phenological stage of flowering, is mainly used as a technique to control yield and improve grape health, aimed at increasing the quality of the wine.

The wine aroma is constituted by hundred of volatile chemical compounds that depend on many viticultural and oenological factors.

Fungus-resistant grape varieties (frgv) are an important field of research in viticulture, as they represent a way of reducing the use of copper-containing pesticides and thus minimising the environmental impact. The literature suggests that resistant grape varieties are a promising solution to the problem of using copper-containing pesticides in viticulture and that their quality has improved in recent years. However, there are still challenges in the acceptance and dissemination of FRGV by wine producers and consumers.

Pulsed electric fields (pef) technology holds significant promise for the agrifood industry, considering the capacity of inducing cell electroporation, due to the disruption of cellular membranes. Pef-induced permeabilization is dependent of the chosen treatment protocol (i.e. Pulse shape, electrical field strength, specific energy) and of the matrix’s characteristics (i.e. Cell radii and size, ph, electrical conductivity).