Phenological stage dependency of Cabernet Sauvignon and Grenache response to water and nutrient limitation 

Malolactic fermentation (MLF) is a secondary wine fermentation conducted by lactic acid bacteria (LAB). This fermentation is important in winemaking as it deacidifies the wine, converting L-malic acid into L-lactic acid and carbon dioxide, and it contributes to microbial stability. Wine pH is highly selective, and at pH below 3.5 generally only strains of O. oeni can survive and express malolactic activity, while under more favorable growth conditions above pH 3.5, species of Lactobacillus and Pediococcus may conduct the MLF. Among the LAB species Lactiplantibacillus plantarum strains have shown most interesting results under hot climate conditions, not only for their capacity to induce MLF, but also for their homo-fermentative properties towards hexose sugars, which makes them suitable for induction of MLF in high pH and high alcohol wines, when inoculated at the beginning of alcoholic fermentation.

Murcia is the Spanish region that produces and exports the largest amount of table grapes, with some European countries consuming a large proportion of the 168,000 tons produced.

Flow cytometry (FCM) is a powerful technique allowing the detection, characterization and quantification of microbial populations in different fields of application (medical environment, food industry, enology, etc.). Depending on the fluorescent markers and specific probes used, FCM provides information on the physiological state of the cell and allows the quantification of a microorganism of interest within a mixed population. For 15 years, the enological sector has shown growing interest in this technique, which is now used to determine the populations present (of interest or spoilage) and the physiological state of microorganisms at the different stages of winemaking.

The decision for grape berries to ripen involves a complex interplay of genetic regulation and environmental cues. This review explores the molecular mechanisms underlying the transition from vegetative growth to ripening, focusing on transcriptomic studies and the role of the NAC gene family. Transcriptomic analyses reveal a significant rearrangement of gene expression patterns during this transition, with up-regulation of ripening-related genes and down-regulation of those associated with vegetative growth. A molecular phenology scale providing a high-precision map of berry transcriptomic development, indicates that key molecular changes occur well before the onset of ripening.

The French grapevine breeding program for durable resistance to downy and powdery mildew (INRAE-ResDur) was initiated more than 20 years ago to help reduce the heavy use of plant protection products and provide a durable mean to cope with a strong pathogen pressure. This program has now proved to be effective, with about ten new varieties already officially registered. However, there is still a lot to be done (1) to reduce the duration of each breeding cycle, (2) to diversify disease factors’ pyramiding and anticipate emerging diseases, (3) to work towards larger adoption of the new resistant varieties. New breeding schemes incorporating for example genomic prediction of breeding values are being evaluated to accelerate genetic gains, saving cost and time while handling complex traits.