terclim by ICS banner
IVES 9 IVES Conference Series 9 The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

Abstract

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘CandidatusPhytoplasma solani’. In symptomatic grapevines cv. ‘Zweigelt’ infected with ‘Ca. P. solani’ compared with uninfected grapevines, metabolic pathways associated with phosphorylated sugar production were induced both at the transcriptional level and at the level of activity of the corresponding enzymes (Dermastia et al., 2021, Int. J. Mol. Sci.22: 3531). In particular, the expression of gene coding for phosphoglucoisomerase was upregulated, resulting in increased phosphoglucoisomerase enzyme activity. Phosphoglucoisomerase converse glucose-1-phosphate to glucose-6-phosphate, which can be used as a substrate for starch biosynthesis. Besides, phosphoglucomutase activity was induced also in Nicotiana benthamiana leaves transiently transformed with the construct of putative effector PoStoSP28, previously annotated as an antigenic membrane protein StAMP related to interaction of phytoplasma with its insect vector. Using a pull-down assay and in planta co-IP assay, we confirmed that PoStoSP28 interacts with both grapevine phosphoglucomutases. In transiently transformed N. benthamiana leaves, PoStoSP28 was localized in the nucleus and cytosol and accompanied by a distinct border at the periphery or just outside the nucleus and in the thread-like structures spanning the cells. Upon closer inspection, some autophagosome-like structures were found in N. benthamiana cells expressing the PoStoSP28 effector. Moreover, PoStoSP28 was not only localized in the autophagosome but also increased the occurrence of autophagosomes (Dermastia et al., 2023, Front. Plant Sci. 14: 1232367). Therefore, the results suggest that PoStoSP28 plays a role in the pathogenicity of phytoplasma in grapevine by interacting with grapevine phosphoglucomutase enzymes.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Maruša Pompe Novak1,2*, Rebeka Strah1,3, Špela Tomaž1,3, Tjaša Lukan1, Anna Coll1, Maja Zagorščak1, Kristina Gruden1, Günter Brader4, Marina Dermastia1

1 Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
2 Faculty of Viticulture and Enology, University of Nova Gorica, Vipava, Slovenia
3 Jožef Stefan International Postgraduate School, Ljubljana, Slovenia
4 Bioresources Unit, Health & Environment Department, Austrian Institute of Technology, Tulln, Austria

Contact the author*

Keywords

grapevine, Candidatus Phytoplasma solani, effector, StAMP, phosphoglucomutase

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Les effets du terroir ou l’expression des potentiels à valoriser

Research into the effects of the Terroir is of major interest for the wine sector. The study of Terroir-Vine-Grape relations, even if it is complex, is fundamental for all viticulture: indeed, the quality of the grape must be the result of the most reasoned agro-viticultural management of the vine possible, which must first, to respect a production balance. The goal sought by the winegrower is to obtain a wine, the optimized result of the interactions Terroir-Grape variety. This link to the terroir is therefore essential to establish by taking into account on the one hand the behavior of the vine (which is the cause), and on the other hand, its effects on the grapes and finally on the wine.

New oenological criteria for selecting strains of Lachancea thermotolerans for wine technology

The study conducted various fermentations of different grape juices using various strains of Lachancea thermotolerans and one strain of Saccharomyces cerevisiae. Because of the new conditions caused by climate change, wine acidity must be influenced as well as the volatile profile. Non-Saccharomyces yeasts such as L. thermotolerans are real options to mitigate the impact of climate change in wine production.

Improvement of the red wine AOC Grignolino d’Asti typicality using some technological innovations

L’AOC Grignolino d’Asti (20000 hl environ de production) est un vin de la province de Asti, produit avec le raisin rouge du cépage de même nom originaire du Piémont (Nord-Ouest d’Italie).

Towards multi-purpose valorisation of polyphenols from grape pomace: Pressurized liquid extraction coupled to purification by membrane processes

Grape by-products (including skins, seeds, stems and vine shoots) are rich in health promoting polyphenols. Their extraction from winery waste and their following purification are of special interest to produce extracts with high added value compounds. Meanwhile, the growing concern over environmental problems associated with economic constraints, require the development of environmentally sustainable extraction technologies. The extraction using semi-continuous subcritical water, as a natural solvent at high temperature and high pressure a technology is promising “green” technology that is environmentally friendly, energy efficient and improve the extraction process in plant tissues.

Биотехнология в системе оздоровления и размножения комплексно-устойчивых сортов винограда на Юге России

The production of certified grape planting material is one of the most important problems in the Russian federation. According to the scheme for the production of healthy grape planting material, before being introduced into in vitro culture, the source plants of each variety (Moldova, Augustin, Bart) were individually assessed for typical varietal characteristics and the presence/absence of symptoms of infection by pests.