terclim by ICS banner
IVES 9 IVES Conference Series 9 The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

Abstract

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘CandidatusPhytoplasma solani’. In symptomatic grapevines cv. ‘Zweigelt’ infected with ‘Ca. P. solani’ compared with uninfected grapevines, metabolic pathways associated with phosphorylated sugar production were induced both at the transcriptional level and at the level of activity of the corresponding enzymes (Dermastia et al., 2021, Int. J. Mol. Sci.22: 3531). In particular, the expression of gene coding for phosphoglucoisomerase was upregulated, resulting in increased phosphoglucoisomerase enzyme activity. Phosphoglucoisomerase converse glucose-1-phosphate to glucose-6-phosphate, which can be used as a substrate for starch biosynthesis. Besides, phosphoglucomutase activity was induced also in Nicotiana benthamiana leaves transiently transformed with the construct of putative effector PoStoSP28, previously annotated as an antigenic membrane protein StAMP related to interaction of phytoplasma with its insect vector. Using a pull-down assay and in planta co-IP assay, we confirmed that PoStoSP28 interacts with both grapevine phosphoglucomutases. In transiently transformed N. benthamiana leaves, PoStoSP28 was localized in the nucleus and cytosol and accompanied by a distinct border at the periphery or just outside the nucleus and in the thread-like structures spanning the cells. Upon closer inspection, some autophagosome-like structures were found in N. benthamiana cells expressing the PoStoSP28 effector. Moreover, PoStoSP28 was not only localized in the autophagosome but also increased the occurrence of autophagosomes (Dermastia et al., 2023, Front. Plant Sci. 14: 1232367). Therefore, the results suggest that PoStoSP28 plays a role in the pathogenicity of phytoplasma in grapevine by interacting with grapevine phosphoglucomutase enzymes.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Maruša Pompe Novak1,2*, Rebeka Strah1,3, Špela Tomaž1,3, Tjaša Lukan1, Anna Coll1, Maja Zagorščak1, Kristina Gruden1, Günter Brader4, Marina Dermastia1

1 Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
2 Faculty of Viticulture and Enology, University of Nova Gorica, Vipava, Slovenia
3 Jožef Stefan International Postgraduate School, Ljubljana, Slovenia
4 Bioresources Unit, Health & Environment Department, Austrian Institute of Technology, Tulln, Austria

Contact the author*

Keywords

grapevine, Candidatus Phytoplasma solani, effector, StAMP, phosphoglucomutase

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Metschnikowia pulcherrima as biocontrol agent in white winemaking

Biocontrol using non-Saccharomyces yeasts is an alternative strategy to chemical additives to prevent the growth of spoilage microorganisms.

Towards a better understanding of the root system diversity and plasticityin young grafted vines using 2D imaging and 3D modelling tools

Three-dimensional functional-structural root architecture models, which decompose the root system architecture (RSA) into elementary developmental processes such as root emission, axial growth, branching patterns and tropism have become useful tools for (i) reconstructing in silico the spatial and temporal dynamics of root systems in a soil volume, (ii) analyzing their genotypic diversity and plasticity to the environment, and (iii) overcoming the bottleneck associated with their visualization and measurement in situ. Here, we present an original work on RSA phenotyping and modelling in grapevine. First, we developed 2D image-based analysis pipelines to quantify morphological and architectural traits in young grafts. Second, we parametrized and validated the 3D root model Archisimple on two rootstock genotypes (RGM, 1103P) grafted with V. vinifera Cabernet-Sauvignon and grown in different controlled conditions (rhizotrons, pots, tubes).

Evaluation of spraying effects of plant protection unmanned aerial vehicle on two different training systems of vine in Northeast China

In recent years, the application of plant protection unmanned aerial vehicle (UAV) in agricultural pest control has become more and more popular. However, there are few reports about the application of plant protection UAV for wine grapes, and there are no studies comparing the spraying effect of plant protection UAV with that of manual operation in vineyards. In this context, the objective of this study was to explore the feasibility of using plant protection UAV in vineyards instead of manual operations by evaluating the effectiveness of UAV spray in two common grape training systems in Northeast China.

Juvenile-to-adult vegetative phase transition in grapevine 

The sequential activity of miR156 and miR172 controls the juvenile to adult phase transition in many plant species, where miR156 abundance decreases while miR172 increases along plant development. Very little is known about phase transition in horticultural woody species, which show substantially long vegetative phases. In grapevine, phase transition seems to be dissociated, displaying a first transition from juvenile to adult vegetative state in the first year, coincident with tendril differentiation and a subsequent induction of inflorescences in place of some of tendrils in later years under flowering inductive environmental conditions. Since grapevine is a highly heterozygous species, the generation of genetically homogeneous material for replicated transcriptomic analyses from seed-derived plants was a main challenge.

Influence of two yeast strains and different nitrogen nutrition on the aromatic compounds in Lugana wine

Lugana Protected Designation of Origin (PDO) wines are made from Turbiana grapes. The aroma of Lugana wines results from the combined contribution of esters, terpenes, norisprenoids, sulfur compounds and the benzenoid methyl salicylate. This study aims to investigate how volatile aroma compounds are affected by different nitrogen supplies and yeast strains. Wines were produced with a standard protocol with 2021 Turbiana grapes with two different yeasts Zymaflore Delta e Zymaflore X5 (Laffort, France).During the alcoholic fermentation of the must, when H2S appeared, additions of various nitrogen supply were made: inorganic nitrogen, organic nitrogen, a mix of inorganic and organic nitrogen and organic nitrogen with an addition of pure methionine. During wine fermentation, a daily measurement of hydrogen sulfide was carried out.