terclim by ICS banner
IVES 9 IVES Conference Series 9 The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

Abstract

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘CandidatusPhytoplasma solani’. In symptomatic grapevines cv. ‘Zweigelt’ infected with ‘Ca. P. solani’ compared with uninfected grapevines, metabolic pathways associated with phosphorylated sugar production were induced both at the transcriptional level and at the level of activity of the corresponding enzymes (Dermastia et al., 2021, Int. J. Mol. Sci.22: 3531). In particular, the expression of gene coding for phosphoglucoisomerase was upregulated, resulting in increased phosphoglucoisomerase enzyme activity. Phosphoglucoisomerase converse glucose-1-phosphate to glucose-6-phosphate, which can be used as a substrate for starch biosynthesis. Besides, phosphoglucomutase activity was induced also in Nicotiana benthamiana leaves transiently transformed with the construct of putative effector PoStoSP28, previously annotated as an antigenic membrane protein StAMP related to interaction of phytoplasma with its insect vector. Using a pull-down assay and in planta co-IP assay, we confirmed that PoStoSP28 interacts with both grapevine phosphoglucomutases. In transiently transformed N. benthamiana leaves, PoStoSP28 was localized in the nucleus and cytosol and accompanied by a distinct border at the periphery or just outside the nucleus and in the thread-like structures spanning the cells. Upon closer inspection, some autophagosome-like structures were found in N. benthamiana cells expressing the PoStoSP28 effector. Moreover, PoStoSP28 was not only localized in the autophagosome but also increased the occurrence of autophagosomes (Dermastia et al., 2023, Front. Plant Sci. 14: 1232367). Therefore, the results suggest that PoStoSP28 plays a role in the pathogenicity of phytoplasma in grapevine by interacting with grapevine phosphoglucomutase enzymes.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Maruša Pompe Novak1,2*, Rebeka Strah1,3, Špela Tomaž1,3, Tjaša Lukan1, Anna Coll1, Maja Zagorščak1, Kristina Gruden1, Günter Brader4, Marina Dermastia1

1 Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
2 Faculty of Viticulture and Enology, University of Nova Gorica, Vipava, Slovenia
3 Jožef Stefan International Postgraduate School, Ljubljana, Slovenia
4 Bioresources Unit, Health & Environment Department, Austrian Institute of Technology, Tulln, Austria

Contact the author*

Keywords

grapevine, Candidatus Phytoplasma solani, effector, StAMP, phosphoglucomutase

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

The anthocyanin profile of galician endangered varieties. A tool for varietal selection

AIM: The current loss of genetic grapevine diversity is mainly due to the reduced number of varieties used for making wine. A way of preserved endangered varieties is the establishment of germplasm banks.

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.

EVALUATING WINEMAKING APPLICATIONS OF ULTRAFILTRATION TECHNOLOGY

Ultrafiltration is a process that fractionates mixtures using semipermeable membranes, primarily on the basis of molecular weight. Depending on the nominal molecular weight cut-off (MWCO) specifications of the membrane, smaller molecules pass through the membrane into the ‘permeate’, while larger molecules are retained and concentrated in the ‘retentate’. This study investigated applications of ultrafiltration technology for enhanced wine quality and profitability. The key objective was to establish to what extent ultrafiltration could be used to manage phenolic compounds (associated with astringency or bitterness) and proteins (associated with haze formation) in white wine.

qNMR metabolomics a tool for wine authenticity and winemaking processes discrimination

qNMR Metabolomic applied to wine offers many possibilities. The first application that is increasingly being studied is the authentication of wines through environmental factors such as geographical origin, grape variety or vintage (Gougeon et al., 2019).

One-year aging of a Sangiovese red wine in tanks of different materials: effect on chemical and sensory characteristics

The aim of this study was to evaluate how the different tank materials could affect the chemical and sensory characteristics of a Sangiovese red wine during one-year aging.