terclim by ICS banner
IVES 9 IVES Conference Series 9 The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

The role of phytoplasma effector interaction with phosphoglucomutase in the pathogenicity of ‘Candidatus Phytoplasma solani’ in grapevine 

Abstract

Bois noir is the most widespread phytoplasma grapevine disease in Europe. It is associated with ‘CandidatusPhytoplasma solani’. In symptomatic grapevines cv. ‘Zweigelt’ infected with ‘Ca. P. solani’ compared with uninfected grapevines, metabolic pathways associated with phosphorylated sugar production were induced both at the transcriptional level and at the level of activity of the corresponding enzymes (Dermastia et al., 2021, Int. J. Mol. Sci.22: 3531). In particular, the expression of gene coding for phosphoglucoisomerase was upregulated, resulting in increased phosphoglucoisomerase enzyme activity. Phosphoglucoisomerase converse glucose-1-phosphate to glucose-6-phosphate, which can be used as a substrate for starch biosynthesis. Besides, phosphoglucomutase activity was induced also in Nicotiana benthamiana leaves transiently transformed with the construct of putative effector PoStoSP28, previously annotated as an antigenic membrane protein StAMP related to interaction of phytoplasma with its insect vector. Using a pull-down assay and in planta co-IP assay, we confirmed that PoStoSP28 interacts with both grapevine phosphoglucomutases. In transiently transformed N. benthamiana leaves, PoStoSP28 was localized in the nucleus and cytosol and accompanied by a distinct border at the periphery or just outside the nucleus and in the thread-like structures spanning the cells. Upon closer inspection, some autophagosome-like structures were found in N. benthamiana cells expressing the PoStoSP28 effector. Moreover, PoStoSP28 was not only localized in the autophagosome but also increased the occurrence of autophagosomes (Dermastia et al., 2023, Front. Plant Sci. 14: 1232367). Therefore, the results suggest that PoStoSP28 plays a role in the pathogenicity of phytoplasma in grapevine by interacting with grapevine phosphoglucomutase enzymes.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Article

Authors

Maruša Pompe Novak1,2*, Rebeka Strah1,3, Špela Tomaž1,3, Tjaša Lukan1, Anna Coll1, Maja Zagorščak1, Kristina Gruden1, Günter Brader4, Marina Dermastia1

1 Department of Biotechnology and Systems Biology, National Institute of Biology, Ljubljana, Slovenia
2 Faculty of Viticulture and Enology, University of Nova Gorica, Vipava, Slovenia
3 Jožef Stefan International Postgraduate School, Ljubljana, Slovenia
4 Bioresources Unit, Health & Environment Department, Austrian Institute of Technology, Tulln, Austria

Contact the author*

Keywords

grapevine, Candidatus Phytoplasma solani, effector, StAMP, phosphoglucomutase

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A GIS Analysis of New Zealand Terroir

This paper summarises a national survey of the geological setting of vineyards in New Zealand. We also provide an overview of climate, slope, aspect and varietals planted in New Zealand vineyards as a whole and for some individual regions.

Sensory profile of wines obtained from disease-resistant varieties in La Rioja

The European wine industry is facing multiple challenges derived from climate change and the pressure of different fungal diseases that are compromising the production of traditional varieties. A sustainable alternative maybe the adoption of resistant varieties.
In this study, we have evaluated the enological potential of 9 resistant varieties (5 white and 4 red varieties) in La Rioja. Microvinifications were carried out with three biological replications. Oenological parameters were very diverse with acid content varying from 2.6 g/L to 6.6 g/L.

The influence of the soil on the phenolic composition of both grapes and wines : “the Grenache observatory”

La composition fine des raisins de Grenache noir est mal connue. Il est généralement admis une certaine variabilité de comportement de ce cépage qui se manifeste principalement sur la couleur des vins. De nombreux facteurs peuvent être à l’origine de cette variabilité : matériel végétal, pratiques culturales, types de vinification et terroir. Un travail de recherche concernant ce cépage a été engagé dans la Vallée du Rhône.

Impacts of fumaric acid addition at the bottling on Cabernet Sauvignon wine quality. Comparison with tartaric acid addition.

Climate change and reduction of inputs are two major challenges for viticulture and oenology. With increasing temperature, wines become less acid and microbiologically less stable (1).

Heatwaves impacts on grapevine physiology, berry chemistry & wine quality

Climate change impacts on both yields and quality have increased over the past decades, with the effects of extreme climate events having the most dramatic and obvious impacts. Increasing length and intensity of heatwaves associated with increased water stress necessitates a reevaluation of climate change responses of grapevine and, ultimately, a reconsideration of vineyard management practices under future conditions. Here we summarize results from a three-year field trial manipulating irrigation prior to and during heatwave events to assess impacts of water application rates on vine health and physiology, berry chemistry, and wine quality. We also highlight potential mitigation strategies for extreme heat, both in terms of water application, as well as other cultural practices that could be widely applicable.