terclim by ICS banner
IVES 9 IVES Conference Series 9 Physiological means to curb the enthusiasm of viruses from infecting grapevines  

Physiological means to curb the enthusiasm of viruses from infecting grapevines  

Abstract

The two most deadly viruses infecting and threatening the productivity of grapevines worldwide are leafroll and red blotch viruses. There is no cure for viral diseases other than roguing the symptomatic vines and replacing them with certified vines derived from clean, virus-tested stocks. 
Given that phloem plays a central role in virus infection, this study aimed to purge the virus by girdling the phloem of leafroll-infected vines at different phenological stages of infected grapevines. Phloem-girdling was performed on canes at veraison to varying regions between the proximal and distal clusters. The vines responded to gridling by forming a callus to bridge the gap and restore vascular functionality. The whole lamina of the leaves above the girdled region turned red due to anthocyanin accumulation triggered by sugars confined to the leaf. This reddening was quite different from the reddening that typically occurs in the leafroll-infected vines, wherein the whole lamina turned red except for the lamina close to the major and minor veins, giving the impression of green veins. The girdled canes showed a low virus concentration compared to the ungirdled canes. Also, the primary metabolites, such as sugars, acids, and nitrogenous compounds, and the secondary metabolites, such as flavanols (tannins), flavonols, and anthocyanins desired for making wine did not vary much between the girdled and ungirdled shoots. This study showed that by girdling the phloem over several growing seasons can reduce the virus load in the infected vines, restoring the vine’s health.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Bhaskar Bondada*

Washington State University Tri-Cities, Wine Science Center, Richland, WA, USA

Contact the author*

Keywords

Acids, Anthocyanins, callus, girdling, phenolics, phloem, sugars

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Terroir and climate: the role of homoclime matching

Climate is an important component or determinant of terroir, especially at the regional level. One can define three levels of terroir. These are the macro– or regional scale, which applies over tens of kilometres of the landscape. The second level is the meso- scale, which applies over kilometres or hundreds of meters, at the individual vineyard scale.

Deciphering the color of rosé wines using polyphenol targeted metabolomics

The color of rosés wines is extremely diverse and a key element in their marketing. It is due to the presence of red anthocyanins extracted from grape skins and pigments formed from them and other wine constituents during wine-making.

Novel analytical technologies for wine fingerprinting in and beyond the laboratory

For characterization, sensory designing and authentication rapid analytical technologies have become available. Some, like Proton Transfer Reaction Mass Spectrometry allow a rapid spectrum of the volatile compounds of wines. Combined with chemometrics wines can be characterized. The same approach can be used to calculate the results of virtual mixtures and allow formulation of constant quality blends. Other new techniques and portable devices based on spectroscopy allow measurements on production sites and in grocery stores, even for the smart consumer. We will present some examples of the application of these techniques for authentication of wines, both in the laboratory and on site.

Caratteristiche fisico-chimiche dei suoli coltivati a vite e loro influenza nella diffusione del mal dell’esca

Il mal dell’esca é una malattia della vite della quale sono state studiate sintomatologia, eziologia, patogenesi ed epidemiologia. Essendo una malattia che colpisce soprattutto la parte epigea delle piante, le caratteristiche dei suoli non sono mai state considerate fra le responsabili della sua insorgenza e diffusione.

Development of a LC-FTMS method to quantify natural sweeteners in red wines

The quality of a wine is largely related to the balance between its sourness, bitterness and sweetness. Recently, molecules coming from grapes have been showed to notably contribute to sweet taste of dry wines. To study the viticultural and oenological parameters likely to affect their concentration, their quantification appears of high interest and subsequently requires powerful analytical techniques. Therefore, a new method using liquid chromatography coupled to high resolution mass spectrometry (LC-HRMS) was developed and validated to quantify epi-DPA-3′-O-β-glucopyranoside acid (epi-DPA-G) and astilbin, sweet molecules identified in wine. Three gradients were tested on five different C18 columns (Hypersil Gold, HSS T3, BEH, Syncronis and Kinetex).