terclim by ICS banner
IVES 9 IVES Conference Series 9 Challenges for the Implementation of commercial inoculum of arbuscular fungi in a commercial Callet vineyard (Vitis vinifera L.)

Challenges for the Implementation of commercial inoculum of arbuscular fungi in a commercial Callet vineyard (Vitis vinifera L.)

Abstract

Over the past 70 years, scientific literature has consistently illustrated the advantageous effects of arbuscular mycorrhiza fungi (AMF) on plant growth and stress tolerance. Recent reviews not only reaffirm these findings but also underscore the pivotal role of AMF in ensuring the sustainability of viticulture. In fact, various companies actively promote commercial inoculants based on AMF as biofertilizers or biostimulants for sustainable viticulture. However, despite the touted benefits of these products, the consistent effectiveness of AMF inoculants in real-world field conditions remains uncertain. Our study investigates the influence of a commercial AMF inoculant containing a mycorrhizal complex of Rhizophagus irregularis and Funneliformis mosseae on a five-year-old vineyard featuring a local grape cultivar (Callet) grafted onto a common rootstock (R110). We monitored the physiological well-being and productivity of inoculated vines compared to control counterparts. Additionally, we assessed the impact of inoculation on the root fungal community determined by NGS of roots DNA from ITS (fungi) regions using Illumina technology. We did not find a statistically significant increase in the photosynthetic rate of inoculated plants, although it did present significantly greater stomatal conductance. Moreover, there were not statistical differences on productivity or grape quality. There was a slight increase in root fungal Shannon diversity in the inoculated plants at beginning of summer but without generating statistically significant differences.  Furthermore, the analysis of the fungal community of the roots, conducted through NMDS with the Bray-Curtis distance, showed no detectable changes in the fungal community after inoculation.

Numerous studies highlight the context-dependent nature of AMF inoculation’s effects, making it challenging to predict outcomes in field conditions. Failures encountered in trials like ours contribute valuable information to the scientific literature, aiding in the determination of prerequisites for effective biofertilizer use in commercial agriculture. Ultimately, the effectiveness of AMF-based biofertilizers remains contingent on specific conditions, highlighting the need for additional research to ensure their consistent and reliable application.

Funding: PID2021-125575OR-C22 project funded by MCIN/AEI/10.13039/501100011033/ and FEDER Una manera de hacer Europa

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Elena Baraza 1,2, Joshua Borras 1, Arantzazu Molins 1.2, and Josefina Bota* 1,2  

1 Research Group on Plant Biology under Mediterranean Conditions, Departament de Biologia, Universitat de les Illes Balears (UIB)
2 Agro-Environmental and Water Economics Institute (INAGEA). Carretera de Valldemossa Km 7.5, 07122 Palma, Balearic Islands, Spain

Contact the author*

Keywords

Arbuscular mycorrhiza fungi (AMF), Biofertilizer, Effectiveness, NGS (Next-Generation Sequencing), Root fungal community, Sustainability

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Aroma characterization of mold resistant base wines for sparkling wine produced in a warm-temperate area at two different altitudes

In a recent context where consumers pay an increasing attention to sustainability and eco-friendly aspects in the decision-making process, the use of the resistant varieties in the wine sector have returned to the attention. In this context, the use of mould-resistant grape varieties would be an opportunity for sparkling wine producers as it can reduced the pesticide utilization in grape management and hence production costs.
However, the use of the resistant varieties to produce the base wine may be strongly influenced due to its requirements for a particular balance between sugars and acidity to ensure the quality of the final product. In addition, the aromatic profile of base wine plays a crucial role in the perception of the quality of the sparkling wine.

The ampelographic collection – glorious past, challenging present, expectant future

During the more than 190 years since the founding of the first ampelographic collection, the creation of a series of collections is attested on the territory of the Republic of Moldova, each operating in different historical periods and socio-economic conditions,

Hierarchy of the interactions between physical and biological parameters intervening in the Pyrenean Gascon foothill vineyard

Un travail sur les A.O.C. du piémont pyrénéen occidental permet de construire une hiérarchie de paramètres climatiques, géo-pédologiques, morphologiques, de saisir leurs niveaux d’interaction et d’élaborer une méthodologie pour proposer un zonage

Identifying physiological and genetic bases of grapevine adaptation to climate change with maintained quality: Genome diversity as a driver for phenotypic plasticity  (‘PlastiVigne’ project)

In the face of climate change, new grapevine varieties will have to show an adaptive phenotypic plasticity to maintain production with erratic water resources, and still ensure the quality of the final product. Their selection requires a better knowledge of the genetic basis of those traits and of the elementary processes involved in their variability. ‘PlastiVigne’, an emblematic project of the Vinid’Occ key challenge, funded by the Occitanie Region (France), tackles this issue with innovative genomic and physiological tools implemented on a unique panel of grape genetic resources representing the genetic diversity of Vitis vinifera. A graph-pangenome is developed from a representative set of high-quality genomes to study the extent and impact of structural genome variations and chromosomal rearrangements in the rapid adaptation capacity of grapevine.

Optimizing protocol for a rapid and cost effective DNA isolation for Marker Assisted Selection pipeline

Grapevine is a plant that holds significant socioeconomic importance due to its production of grapes for fresh consumption, wines, and juices. However, climate changes and susceptibility to diseases pose a threat to the quality and yield of these products. The breeding of new genotypes that are resistant/tolerant to biotic and abiotic stresses is essential to overcome the impact of climate changes. In this regard, Marker-assisted selection (MAS), which uses DNA markers, is a crucial tool in breeding programs. The efficiency and economy of this method depend on finding rapid DNA isolation methods.