terclim by ICS banner
IVES 9 IVES Conference Series 9 Challenges for the Implementation of commercial inoculum of arbuscular fungi in a commercial Callet vineyard (Vitis vinifera L.)

Challenges for the Implementation of commercial inoculum of arbuscular fungi in a commercial Callet vineyard (Vitis vinifera L.)

Abstract

Over the past 70 years, scientific literature has consistently illustrated the advantageous effects of arbuscular mycorrhiza fungi (AMF) on plant growth and stress tolerance. Recent reviews not only reaffirm these findings but also underscore the pivotal role of AMF in ensuring the sustainability of viticulture. In fact, various companies actively promote commercial inoculants based on AMF as biofertilizers or biostimulants for sustainable viticulture. However, despite the touted benefits of these products, the consistent effectiveness of AMF inoculants in real-world field conditions remains uncertain. Our study investigates the influence of a commercial AMF inoculant containing a mycorrhizal complex of Rhizophagus irregularis and Funneliformis mosseae on a five-year-old vineyard featuring a local grape cultivar (Callet) grafted onto a common rootstock (R110). We monitored the physiological well-being and productivity of inoculated vines compared to control counterparts. Additionally, we assessed the impact of inoculation on the root fungal community determined by NGS of roots DNA from ITS (fungi) regions using Illumina technology. We did not find a statistically significant increase in the photosynthetic rate of inoculated plants, although it did present significantly greater stomatal conductance. Moreover, there were not statistical differences on productivity or grape quality. There was a slight increase in root fungal Shannon diversity in the inoculated plants at beginning of summer but without generating statistically significant differences.  Furthermore, the analysis of the fungal community of the roots, conducted through NMDS with the Bray-Curtis distance, showed no detectable changes in the fungal community after inoculation.

Numerous studies highlight the context-dependent nature of AMF inoculation’s effects, making it challenging to predict outcomes in field conditions. Failures encountered in trials like ours contribute valuable information to the scientific literature, aiding in the determination of prerequisites for effective biofertilizer use in commercial agriculture. Ultimately, the effectiveness of AMF-based biofertilizers remains contingent on specific conditions, highlighting the need for additional research to ensure their consistent and reliable application.

Funding: PID2021-125575OR-C22 project funded by MCIN/AEI/10.13039/501100011033/ and FEDER Una manera de hacer Europa

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Elena Baraza 1,2, Joshua Borras 1, Arantzazu Molins 1.2, and Josefina Bota* 1,2  

1 Research Group on Plant Biology under Mediterranean Conditions, Departament de Biologia, Universitat de les Illes Balears (UIB)
2 Agro-Environmental and Water Economics Institute (INAGEA). Carretera de Valldemossa Km 7.5, 07122 Palma, Balearic Islands, Spain

Contact the author*

Keywords

Arbuscular mycorrhiza fungi (AMF), Biofertilizer, Effectiveness, NGS (Next-Generation Sequencing), Root fungal community, Sustainability

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

A telomere-to-telomere genome assembly of Vitis vinifera cv. Glera to support grapevine breeding and genetic research

Grapevine (Vitis vinifera L.) is a clonally propagated crop of high economic value and remarkable genetic complexity, characterized by extensive structural variation and heterozygosity.

The combined effects of climate, soils, and deficit irrigation on yield and quality of Touriga Nacional under high atmospheric demand in the Douro Region

Global warming is one of the biggest environmental, social and economic threats in several viticultural regions. In the Douro Valley, changes are expected in the coming years, namely an increase in temperature and a decrease in precipitation. These changes are likely to have consequences for the production and quality of wine.
The aim of this study was to explore the effects of different soil characteristics combined with several deficit irrigation strategies, managed throughout ETc references and predawn leaf water potentials thresholds, on physiology, yield, and qualitative attributes on the Touriga Nacional variety under years of mild to severe water and heat stress.
The studies were conducted over seven years (2015 to 2021) in two plots of a commercial vineyard located at Quinta do Ataíde (Symington Family Estates) planted in 2011 and 2014 at 170 meters elevation, growing under three water regimes: non-irrigated (NI) and two deficit irrigation strategies (30% and 60% ETc) assessed weekly by Ψpd. The site has an annual rainfall below 500 mm, with high atmospheric demand. Climate data was collected from a weather station, located on site. Berry ripening was followed weekly for fruit analysis. At harvest, yield, vigour and pruning weight per vine were determined from 90 vines by treatment. Each season at veraison the NDVI Index was accessed by a drone. The soils physic-chemistry in the experimental blocs were analysed and grouped by SWHC. Delta C-13 analyses were also performed per treatment in two years.Irrigation had a positive effect on yield per vine, mostly due to an increase in berry and cluster weight, and fertility index through the years. A significant increase in sugar content, colour and phenols was observed with deficit irrigation in some years, but vine vigour related to soil characteristics had by far the greatest impact on quality.

Impact of polyclonal selection for abiotic stress tolerance on the yield and must quality traits of grapevine varieties

The effects of climate change in viticulture are currently a major concern, with heat waves and drought affecting yield, wine quality, and in extreme cases, even plant survival. Ancient grapevine varieties have high intravarietal genetic variability that so far has been explored successfully to improve yield and must quality. Currently, there is little information available on intravarietal variability regarding responses to stress. In the current work, the intravarietal genetic variability of several Portuguese varieties was studied for yield, must quality, and tolerance to abiotic stress, through indirect, rapid, and nondestructive measurements carried out in the field.

“Garrigues”, part of the mediterranean vine terroirs

Les paysages viticoles méditerranéens présentent une originalité qui ne se retrouve nulle part ailleurs : ils associent des garrigues très odoriférantes à des parcelles de vignes souvent qualitatives. La connaissance empirique des vins du Languedoc par leurs dégustateurs a conduit la Chambre d’Agriculture de l’Hérault à supposer que les arômes de la garrigue environnante peuvent se retrouver dans les vins (arômes de ciste, de genévrier, … )

Use of fumaric acid to control pH and inhibit malolactic fermentation in wines

In this audio recording of the IVES science meeting 2022, Antonio Morata (Universidad Politécnica de Madrid, Madrid, Spain) speaks about the use of fumaric acid to control pH and inhibit malolactic fermentation in wines.