terclim by ICS banner
IVES 9 IVES Conference Series 9 Improving shelf life of viticulture-relevant biocontrol and biostimulant microbes using CITROFOL® AI as liquid carrier

Improving shelf life of viticulture-relevant biocontrol and biostimulant microbes using CITROFOL® AI as liquid carrier

Abstract

Bacillus velezensis and Trichoderma harzianum are relevant microorganisms used in viticulture as biocontrol agents against pathogens of trunk (e.g. Phaeoacremonium minimum), leaves (e.g. Plasmopara viticola) or fruit (e.g. Botrytis cinerea), or as biostimulants, improving the resilience of plants against biotic or abiotic stressors through different direct and non-direct interactions.
In this biotechnological approach, formulation plays a crucial role.  Controlling water activity in the product, thus stabilising microbial viability is key to ensuring effective application.  We present the benefits of the citrate ester CITROFOL® AI (triethyl citrate) as a novel bio-based carrier liquid in microbial formulations. CITROFOL® AI is safe for humans and the environment, thus offering a promising base for sustainable treatments in viticulture.
Commercial products containing B. velezensis and T. harzianum formulated as wettable powders (WP) or suspension concentrates (SC) were used for viability tests and compared with the same microorganisms prepared as dispersion concentrates (DC) 1:10 ration in CITROFOL® AI. The formulations were subjected to a demanding storage test at 40°C for 24 weeks. Microbial viability was monitored by plate counting periodically.
B. velezensis showed a high overall robustness during storage. However, comparing liquid products, the cell viability in the SC formulation declined by approximately one order of magnitude more than in the DC formulation in CITROFOL® AI. T. harzianum generally was highly susceptible during storage. However, the cell viability after 24 weeks was two orders of magnitude higher when formulated as DC in CITROFOL® AI compared to the WP. Thus, CITROFOL® AI improved the shelf life of both microorganisms tested.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Teresa Berninger1, Carolin Stern1, Victoria Sevillano, Óscar González-López2*

1 Jungbunzlauer Ladenburg GmbH
2 University of La Rioja, Department of Food and Agriculture

Contact the author*

Keywords

Formulation, Viticulture, Citrate ester, Biocontrol, Biostimulant

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Chitosan treatment to manage grapevine downy mildew

Downy mildew is one of the most important grapevine diseases, caused by the Oomycete Plasmopara viticola. The management of the disease in organic agriculture can require up to 15 copper applications per year. However, copper accumulates in the soil, is phytotoxic and is toxic for organisms living in the soil, its use has been restricted in European Union to maximum 28 kg in 7 years. Therefore, testing of alternatives with equal effectiveness is desirable. Among those, the natural biopolymer chitosan, obtained from crab shells, proved to be effective toward downy mildew in plot experiments. The aim of our trials was to extend chitosan applications in large scale experiments in different years, cultivars and environmental conditions.

Cultivo de la Malvasia en Tenerife

El archipiélago Canario, conocido en el pasado como las Islas del Vino, fue una gran potencia en la elaboración y comercialización del vino, sobre todo de caldos elaborados con la variedad Malvasía.

2018 updates on the agronomic performances of fungus resistant wine grapes in Trentino (Italy)

On the market there are several wine grapes which are tolerant to the main fungal diseases. These varieties, commonly defined “resistant”, were developed in the grapevine breeding programs carried out mainly in Germany, France, Hungary and Italy. Some of these cultivars have been included in the national catalogues of wine grape varieties and have sometimes been allowed for specific kinds of wine. The VEVIR project, aimed at the enological evaluation of resistant vines, involves 33 cultivars achieved at the State Institute for Viticulture Freiburg in Germany, the Research Institute of Viticulture and Enology Pecs in Hungary and the Fondazione Edmund Mach S. Michele all’Adige (FEM) in Italy.

Response of different nitrogen supplementation on Saccharomyces cerevisiae metabolic response and wine aromatic profile

The wine yeast Saccharomyces cerevisiae can highly affect wine aromatic profile by producing and/or mediating the release of a whole range of metabolites (such as thiols, esters, and terpenes), which in turn contribute to enhanced aroma and flavor. These metabolites depend on yeast metabolism activated during fermentation which can constitute the ‘’metabolic footprint’’ of the yeast strain that carried out the process.

Application of ethephon induces an increase in berry flavonols leading to an increase in wine blueish hue through copigmentation

Exogenous applications of plant growth regulators are valuable tools to modulate berry ripening and to explore the roles of phytohormones in controlling plant developmental processes.