terclim by ICS banner
IVES 9 IVES Conference Series 9 Improving shelf life of viticulture-relevant biocontrol and biostimulant microbes using CITROFOL® AI as liquid carrier

Improving shelf life of viticulture-relevant biocontrol and biostimulant microbes using CITROFOL® AI as liquid carrier

Abstract

Bacillus velezensis and Trichoderma harzianum are relevant microorganisms used in viticulture as biocontrol agents against pathogens of trunk (e.g. Phaeoacremonium minimum), leaves (e.g. Plasmopara viticola) or fruit (e.g. Botrytis cinerea), or as biostimulants, improving the resilience of plants against biotic or abiotic stressors through different direct and non-direct interactions.
In this biotechnological approach, formulation plays a crucial role.  Controlling water activity in the product, thus stabilising microbial viability is key to ensuring effective application.  We present the benefits of the citrate ester CITROFOL® AI (triethyl citrate) as a novel bio-based carrier liquid in microbial formulations. CITROFOL® AI is safe for humans and the environment, thus offering a promising base for sustainable treatments in viticulture.
Commercial products containing B. velezensis and T. harzianum formulated as wettable powders (WP) or suspension concentrates (SC) were used for viability tests and compared with the same microorganisms prepared as dispersion concentrates (DC) 1:10 ration in CITROFOL® AI. The formulations were subjected to a demanding storage test at 40°C for 24 weeks. Microbial viability was monitored by plate counting periodically.
B. velezensis showed a high overall robustness during storage. However, comparing liquid products, the cell viability in the SC formulation declined by approximately one order of magnitude more than in the DC formulation in CITROFOL® AI. T. harzianum generally was highly susceptible during storage. However, the cell viability after 24 weeks was two orders of magnitude higher when formulated as DC in CITROFOL® AI compared to the WP. Thus, CITROFOL® AI improved the shelf life of both microorganisms tested.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Teresa Berninger1, Carolin Stern1, Victoria Sevillano, Óscar González-López2*

1 Jungbunzlauer Ladenburg GmbH
2 University of La Rioja, Department of Food and Agriculture

Contact the author*

Keywords

Formulation, Viticulture, Citrate ester, Biocontrol, Biostimulant

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Zoning for drinking, tasting the territory place (“Great Zonation”): first considerations and methodology

Following the idea of « Grande Filiera » (GF) (Great chain), of « Grande Zonazione » (GZ) (Great Zonation), of “interpretation, estimation and valorisation of vineyards and wines landscape, of “qualities”(we have classified more than ninety), of quality economy.

CHARACTERIZATION AND IDENTIFICATION OF YEAST BIOACTIVE PEPTIDES RELEASED DURING FERMENTATION AND AUTOLYSIS IN MODEL WINE

Aging wine on lees is a consolidated practice during which some yeast components (e.g., polysaccharides,
proteins, peptides) are released and solubilized in wine thus, affecting its stability and quality.
Apart from the widely studied mannoproteins, the role of other yeast components in modulating wine
characteristics is still scarce. Wine peptides have been studied for their contribution to taste, antioxidant,
and antihypertensive potentials. However, the peptides detected in wine can be influenced by the
interaction between yeasts and grape components.

Key phenolic compounds in the pulp of new red-fleshed table grape hybrids: anthocyanins and flavonols 

The cultivated area of table grapes worldwide has experienced a paramount increase over the last two decades. In this current scenario, traditional varieties are being replaced by new cultivars that prioritize a profitable and sustainable agriculture, while satisfying consumer demands. It is widely recognized that wine varieties, especially those with red berry flesh, are renowned for their high antioxidant capacity and phenolic compounds, which promote health. Recently, this topic has also gained significance in table grape breeding programs.

Is complex nutrition more advantageous than mineral nitrogen for the fermentative capacities of S. cerevisiae?

During alcoholic fermentation, nitrogen is an essential nutrient for yeast as it plays a key role in sugar transport and biosynthesis of wine aromatic compounds (thiols, esters, higher alcohols). The main issue of a lack in yeast assimilable nitrogen (yan) in winemaking is sluggish or stuck fermentations promoting the growth of alteration species which may lead to economic losses. However, correcting this nitrogen deficiency is sometimes not enough to restore proper fermentation performance. This suggests the existence of other nutritional shortages.

Under-vine cover crops as a management tool for irrigated Mediterranean vineyards: agronomic implications and changes in soil physical and biological properties 

Cover crops are increasingly considered in Mediterranean climate vineyards due to a combination of agronomic and regulatory considerations. However, the soil under the vines themselves is typically kept free of vegetation by mechanical plowing or herbicide spraying. Taking into account that these practices may convey a number of non-favourable economic and environmental implications, and the fact that drip irrigation can ease the use of cover crops under the vines, the aim of this work was to evaluate the agronomic implications and the changes in soil physical and biological properties caused by an under-vine cover crop in a Mediterranean area.