Optimized protocol for high-quality RNA extraction from grape tissues using sorbitol pre-wash

Grapevine vigour, defined as the propensity to assimilate, store and/or use non-structural sugars for allowing fast growth of shoots and producing large canopies[1], is crucial to optimize vineyard management. Recently, a model has been proposed for predicting the vigor of young grapevines through the measurement of the vegetative growth and physiological parameters, such as water status and gas exchange[2]. Our objectives were (1) to explore the influence of the association of two grapevine varieties (Tempranillo and Cabernet Sauvignon, grafted onto R110 rootstocks) with arbuscular mycorrhizal fungi (AMF) on the vegetative vigour of young plants; and (2) to assess the effect of environmental factors linked to climate change on the vegetative vigour of Cabernet Sauvignon.

The South Western Cape of South Africa is exposed to strong southerly and south easterly synoptic winds during the growth period of the grapevine. The development of sea breezes in the afternoon is also a phenomenon associated with the ripening period of grapes cultivated in this coastal area. Wind is one of the environmental variables having the greatest spatial variation but the implications of regular exposure to wind for the performance of the grapevine has not yet been determined for vineyards in the South Western Cape. This study was initiated to meet this need.
The study was conducted in a hedge-trellised vineyard of Vitis vinifera L. cv Merlot with north east – south west row direction. Thirty experimental sites, each consisting of 14 vines, were identified as being exposed to wind or sheltered based on hand-held anemometer readings during the 2001/2002 season. Four stationary anemometers were strategically positioned between the thirty sites. Stomatal conductance and leaf temperature were measured with a PP systems porometer. Vegetative and yield measurements were performed during the 2002/2003 season. The t-test of equal variance was used to determine significant differences in measured parameters between exposed and sheltered grapevines.
Stomatal conductance and leaf area were significantly reduced by exposure to wind. This was associated with a significant reduction in the leaf area of primary shoots, related to shorter shoots, but a significant augmentation of secondary shoot leaf number and area. The number of bunches per vine and yield were also reduced for exposed vines. The berry potassium content was significantly increased for exposed grapevines.
This demonstrates that exposure to wind can result in significant within-vineyard, and potentially between-vineyard, variability in grapevine physiology, vegetative growth, yield and berry composition, with implications for wine style and quality.

Wooden barrels have been the preferred method for oak maturation for wines, but the use of alternative oak products, such as staves and oak chips have increased in South Africa due to lower production costs. This study investigated the effect of different oak products used during fermentation and ageing on the sensory profile, degree of liking and perceived quality of a South African Chenin blanc wine. The different wine treatments included an unoaked tank control wine, wines matured in 5th fill barrels, wines matured in new barrels from three different cooperages, and wines matured in 5th fill barrels with stave inserts from two different cooperages.

In this audio recording of the IVES science meeting 2022, Thibaut Verdenal (Agroscope, Pully, Switzerland) speaks about managing nitrogen balance in cover-cropped vineyard. This presentation is based on an original article accessible for free on OENO One.

Barrel ageing is a crucial step in the wine process because it allows many changes to the wine as enrichment, colour stabilization, clarification and also a slow oxygenation. Effects of the oak barrel have to be known to prevent oxidation of the wine. The type of bung used during ageing is also a parameter to consider. Ageing sweet wines in barrel is a real challenge. These wines may need some oxygen at the beginning of ageing but they should be protected at the end of their maturation, to avoid oxidation.