Optimized protocol for high-quality RNA extraction from grape tissues using sorbitol pre-wash

Ozone is a potent oxidizing compound that quickly decomposes into oxygen without residues. Previous works reported that ozone is not only a disinfectant that directly harms the pathogens of the vine but also activates systemic defense systems in the plant by activating oxidative stress. We assume these systemic defense mechanisms are essential to the vines’ resistance to downy and powdery mildew (Plasmopara viticola & Erysiphe necator, respectively). The goals of the research are to examine the effect of spraying with ozone water on the plant’s resistance against the mentioned pathogens as well as to characterize the metabolic profile of the plants treated with ozone as well as physiological characteristics in the vines such as the level of Photosynthesis and crop yield. Vines in the vineyard sprayed with ozone water at concentrations of 2 and 4 PPM weekly and biweekly, untreated control & conventional spray. Leaves were taken from vines 2,4,7,9 and 11 days after exposure to ozone and inoculated with the pathogens.

Wine contains secondary metabolites derived from aromatic amino acids (AADC), which can determine quality, stability and bioactivity. Several yeast species, as well as some lactic acid bacteria (LAB), can contribute in the production of these aromatic compounds. Winemaking should be studied as a series of microbial interactions, that work as an interconnected network, and can determine the metabolic and analytical profiles of wine. The aim of this work was to select microorganisms (yeast and LAB) based on their potential to produce AADC compounds, such as tyrosol and hydroxytyrosol, and design a microbial consortium that could increase the production of these AADC compounds in wines.

Field-grown crops such as grapevines, rely on ambient environmental conditions for growth and development.

Cover crops are acknowledged to be an interesting tool to produce
higher quality grapes in red varieties, as they generally reduce vine vigour and yield. However, their incidence in white wine quality is not clear, since higher nitrogen availability can play an important positive
role, and cover crops may compete for this nutrient. The possible reduction in available nitrogen can also modify the fermentation processes, as well as the synthesis of aromas in the wine. The aim of this work was to evaluate the long-term effect of a grass cover crop on grape and wine quality.

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…