terclim by ICS banner
IVES 9 IVES Conference Series 9 In vitro regeneration of grapevine cv. Aglianico via somatic embryogenesis: preliminary studies for next genome editing applications  

In vitro regeneration of grapevine cv. Aglianico via somatic embryogenesis: preliminary studies for next genome editing applications  


Italy is a rich hub of viticultural biodiversity harboring hundreds of indigenous grape varieties that have adapted over centuries to the diverse climatic and geographic conditions of its regions. Preserving this biodiversity is essential for maintaining a diversified genetic pool, crucial for addressing future challenges such as climate change and emerging plant diseases. Rising temperatures, precipitation pattern variations, and extreme weather events can affect grape ripening, crop quality, and contribute to disease development. Integrated disease management necessitates exploration of novel strategies. Biotechnologies emerge as a significant player in tackling modern viticulture challenges. New plant breeding technologies (NpBT) can be employed in grapevine cultivation and, in particular, genome editing through CRISPR/Cas9 system has been shown to be a valid application for targeted mutagenesis, by until now its application is restricted on a few cultivars. Genetic improvement via in vitro delivery of desired constructs requires the regeneration of genome-edited plants. In vitro plant regeneration, a pivotal process in genetic engineering, encounters obstacles, particularly in grapevines, due to factors like genotype and explant-dependent responses. Therefore, reliable in vitro regeneration and propagation systems are imperative. Understanding and enhancing regeneration across different genotypes are critical steps in advancing genetic improvements and gene functional studies in grapevines. The study focuses on developing an efficient in vitro plant regeneration protocol by somatic embryogenesis (SE) of the grapevine cv. Aglianico, the most important grapevine variety of southern Italy regions. The embryogenic materials obtained will be used for future functional studies through genome editing approaches.


Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster


Carmine Carratore1*, Edoardo Bertini2, Luca Cattaneo1, Clizia Villano3, Riccardo Aversano3, Mario Pezzotti1, Sara Zenoni1,2

1 Department of Biotechnology, University of Verona, 37134 Verona, Italy
2 Edivite s.r.l, San Pietro Viminario, Quartiere San Mauro 30, 35020 Padova, Italy
3 Department of Agricultural Sciences, University of Napoli Federico II, 80055 Portici, Italy

Contact the author*


Vitis vinifera, Biodiversity, Genome Editing, Somatic Embryogenesis, Aglianico


IVES Conference Series | Open GPB | Open GPB 2024


Related articles…

Enhanced polyphenol extraction during Pinot Noir and Cabernet Sauvignon wine making

The quality of red wine depends on the composition of polyphenols influencing wine color and taste. The question is, how much we must fear over extraction, especially of seed tannins, under cool climate conditions. The extraction of polyphenols from grape skins and grape seeds were investigated for the grape varieties Cabernet Sauvignon and Pinot noir

Spatial variability of temperature is linked to grape composition variability in the Saint-Emilion winegrowing area

Elevated temperature during the grape maturation period is a major threat for grape quality and thus wine quality. Therefore, characterizing the grape composition response to temperature at a larger scale would represent a crucial step towards adaptation to climate change. In response to changes in temperature, various physiological mechanisms regulate grape composition. Primary and secondary metabolisms are both involved in this response, with well-known effects, for example on anthocyanins, and lesser known effects, for example on aromas or aroma precursors. At the field scale or at the regional scale, however, numerous environmental or plant-specific factors intervene to make the effects of temperature difficult to distinguish from overall variability. In this study, it was attempted to overcome this difficulty by selecting well-characterized situations with differing temperatures.
A long-term study of air temperature variability across several Merlot vineyards in the Saint-Emilion and Pomerol wine producing area found significant temperature differences and gradients at various time scales linked to environmental factors. From this study area, a few sites were selected with similar age, soil and training system conditions, and with repeated and contrasted temperature differences during the maturation period. The average temperature difference during the maturation period was about 2°C between cooler and warmer sites, a difference similar to that expected under future climate change scenarios. In close vicinity to the temperature sensors at each site, grape berries were sampled at different times until full maturity during 2019 and 2020. Also, berries from bunches on either side of the row were analyzed separately, allowing an investigation of bunch exposure effect associated with the coupling of berry temperature and solar radiation. Four replicates of pooled berries for each time – site – bunch exposure combination were obtained and analyzed for biochemical composition. Analyses of variance of the biochemical composition data collected at different sampling times reveal significant effects associated with temperature, site, and bunch azimuth. For instance, anthocyanins in grape skins are clearly influenced by temperature and solar radiation exposure, with up to 30% reduction in warmer conditions.

Methodological advances in relating deep root activity to whole vine physiology

Full understanding of grapevine responses to variable soil resources requires
assessing the grapevine root system. Grapevine root systems are expansive and examining deep roots (i.e., >40 cm)
is particularly important in conditions where grapevines increase reliance on deep soil resources, such as drought
or plant competition. Traditional methods of assessing roots rely on morphological traits associated specific
functions (e.g., root color, diameter, length), while recent methodological advances allow for estimating root
function more directly (e.g., omics). Yet, the potential of applying refined methods remains underexplored for roots
at deep depths.

Relations entre critères sensoriels et analytiques des vins et des vendanges de Cabernet franc issus de terroirs et de millésimes différents en Val de Loire. Essai de caractérisation de la typicité

En France, la notion de Terroir a largement contribué à la réputation de nombreux vignobles. Elle a permis aussi d’accentuer la sensibilité des consommateurs, à la notion d’origine d’un produit. L’avenir de nombreux vignobles français semble lié à la capacité à innover en produisant des vins de qualité possédant en plus une typicité, aspect sensoriel susceptible de s’affirmer comme un facteur de vente auprès des futurs clients éduqués sur le plan du goût.

PIWIs’ variation in drought response under semi-controlled conditions 

Grapevine interspecific hybrids (PIWIs, from German “pilzwiderstandsfähige Rebsorten” meaning fungus tolerant grapevine cultivars), offer a promising alternative to traditional cultivars in many wine regions due to their tolerance to certain fungal diseases. This makes them a potential solution for sustainable wine production, particularly under organic systems. Despite extensive research on certain agronomic traits and susceptibility to biotic diseases, such as powdery and downy mildews, the response of these cultivars to abiotic stressors, such as drought, remains unclear. Our study aims to investigate the eco-physiological traits of two commercial PIWI cultivars, Muscaris and Souvignier gris, at the leaf level to evaluate their response to drought stress.