terclim by ICS banner
IVES 9 IVES Conference Series 9 Optimization of in vitro establishment of grapevine varieties for fast micropropagation 

Optimization of in vitro establishment of grapevine varieties for fast micropropagation 

Abstract

Micropropagation is an important alternative to conventional methods of plant propagation. The objective of this study was to optimize a protocol for in vitro micropropagation of selected grapevine hybrids (H19 and H20) that are included in our breeding program. For the sprouting initiation experiment, nodal cuttings with only one axillary bud from two hybrids were separated, disinfected, and cultivated in 50% Murashige Skoog nutrient medium (½ MS) and Woody Plant Medium (WPM), adding 4.4 µM benzyladenine (BA) in both mediums. To optimize root induction, the sprouts obtained were cultivated in ½ MS and WPM, testing doses of 2, 4 and 8 µM Indole Acetic Acid (IAA) respectively. According to the results, the highest percentage of sprouted buds was obtained in ½ MS + 4.4 µM BA for H19 and H20 (79 and 82%, respectively) at 14 days. At 28 days, the percentage was lower in all of cases. Regarding the rooted sprouts, the highest percentage obtained was 52% in the WPM medium for H19 and 46% in the WPM + 4µM IAA medium for H20 at 14 days. At 28 days, however, the highest percentage of rooted shoots was in ½ MS + 2µM AIA medium for H19 and H20 (89 and 93%, respectively). In conclusion, the best way to micropropagate these hybrids in a short period of time is ½ MS + 4.4 µM BA and WPM for H19, and ½ MS + 4.4µM BA and WPM + 4µM IAA for H20.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

María Isabel Serrano Sánchez1*, Manuel Tornel Martínez1

1 Instituto Murciano de Desarrollo Agrario y Medioambiental (IMIDA). Equipo de Mejora de Uva de Mesa. C/ Mayor, s/n, 30150 Alberca Las Torres, Murcia (Spain)

Contact the author*

Keywords

micropropagation, rooting, in vitro culture, IAA, BA

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Microbiome, disease-resistant varieties, and wine quality

The development of interspecific hybrid varieties (ihvs) resistant to diseases such as powdery mildew and downy mildew allows for a decrease in the use of inputs in vineyards. In this pers-pective, ihvs represent a response to societal demand for reducing environmental impact and are increasingly used in viticulture. At the same time, wines resulting from so-called sponta-neous fermentations, based on indigenous flora, have recently gained popularity.

Genetic traceability of ‘Nebbiolo’ musts and wines by single nucleotide polymorphism (SNP) genotyping assays

AIM: ‘Nebbiolo’ (Vitis vinifera L.) is one of the most ancient and prestigious Italian grape cultivars. It is renowned for its use in producing monovarietal high-quality red wines, such Barolo and Barbaresco. Wine quality and value can be heavily modified if cultivars other than those allowed are employed.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.

From precursor identification to the study of the distribution of 3-methyl-2,4-nonanedione in red wines and spirits

Prematurely aged red wines are marked by intense prune and fig aromatic nuances that dominate the complex bouquet that can be achieved through bottle aging.

Impact of climate variability and change on grape yield in Italy

Viticulture is entangled with weather and climate. Therefore, areas currently suitable for grape production can be challenged by climate change. Winegrowers in Italy already experiences the effect of climate change, especially in the form of warmer growing season, more frequent drought periods, and increased frequency of weather extremes.
The aim of this study is to investigate the impact of climate variability and change on grape yield in Italy to provide winegrowers the information needed to make their business more sustainable and resilient to climate change. We computed a specific range of bioclimatic indices, selected by the International Organisation of Vine and Wine (OIV), and correlated them to grape yield data. We have worked in collaboration with some wine consortiums in northern and central Italy, which provided grape yield data for our analysis.
Using climate variables from the E-OBS dataset we investigate how the bioclimatic indices changed in the past, and the impact of this change on grape productivity in the study areas. The climate impact on productivity is also investigated by using high-resolution convection-permitting models (CPMs – 2.2 horizontal resolution), with the purpose of estimating productivity in future emission scenarios. The CPMs are likely the best available option for this kind of impact studies since they allow a better representation of small-scale processes and features, explicitly resolve deep convection, and show an improved representation of extremes. In our study, we also compare CPMs with regional climate models (RCMs – 12 km horizontal resolution) to assess the added value of high-resolution models for impact studies. Further development of our study will lead to assessing the future suitability for vine cultivation and could lead to the construction of a statistical model for future projection of grape yield.