terclim by ICS banner
IVES 9 IVES Conference Series 9 Optimization of in vitro establishment of grapevine varieties for fast micropropagation 

Optimization of in vitro establishment of grapevine varieties for fast micropropagation 

Abstract

Micropropagation is an important alternative to conventional methods of plant propagation. The objective of this study was to optimize a protocol for in vitro micropropagation of selected grapevine hybrids (H19 and H20) that are included in our breeding program. For the sprouting initiation experiment, nodal cuttings with only one axillary bud from two hybrids were separated, disinfected, and cultivated in 50% Murashige Skoog nutrient medium (½ MS) and Woody Plant Medium (WPM), adding 4.4 µM benzyladenine (BA) in both mediums. To optimize root induction, the sprouts obtained were cultivated in ½ MS and WPM, testing doses of 2, 4 and 8 µM Indole Acetic Acid (IAA) respectively. According to the results, the highest percentage of sprouted buds was obtained in ½ MS + 4.4 µM BA for H19 and H20 (79 and 82%, respectively) at 14 days. At 28 days, the percentage was lower in all of cases. Regarding the rooted sprouts, the highest percentage obtained was 52% in the WPM medium for H19 and 46% in the WPM + 4µM IAA medium for H20 at 14 days. At 28 days, however, the highest percentage of rooted shoots was in ½ MS + 2µM AIA medium for H19 and H20 (89 and 93%, respectively). In conclusion, the best way to micropropagate these hybrids in a short period of time is ½ MS + 4.4 µM BA and WPM for H19, and ½ MS + 4.4µM BA and WPM + 4µM IAA for H20.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

María Isabel Serrano Sánchez1*, Manuel Tornel Martínez1

1 Instituto Murciano de Desarrollo Agrario y Medioambiental (IMIDA). Equipo de Mejora de Uva de Mesa. C/ Mayor, s/n, 30150 Alberca Las Torres, Murcia (Spain)

Contact the author*

Keywords

micropropagation, rooting, in vitro culture, IAA, BA

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Grapevine yield-gap: identification of environmental limitations by soil and climate zoning in Languedoc-Roussillon region (south of France)

Grapevine yield has been historically overlooked, assuming a strong trade-off between grape yield and wine quality. At present, menaced by climate change, many vineyards in Southern France are far from the quality label threshold, becoming grapevine yield-gaps a major subject of concern. Although yield-gaps are well studied in arable crops, we know very little about grapevine yield-gaps. In the present study, we analysed the environmental component of grapevine yield-gaps linked to climate and soil resources in the Languedoc Roussillon. We used SAFRAN data and IGP Pays d’Oc wine yields from 2010 to 2018. We selected climate and soil indicators proving to have a significant effect on average wine yield-gaps at the municipality scale. The most significant factors of grapevine yield were the Soil Available Water Capacity; followed by the Huglin Index and the Climatic Dryness Index. The Days of Frost; the Soil pH; and the Very Hot Days were also significant. Then, we clustered geographical zones presenting similar indicators, facilitating the identification of resources yield-gaps. We discussed the number of zones with the experts of IGP Pays d’Oc label, obtaining 7 zones with similar limitations for grapevine yield. Finally, we analysed the main resources causing yield-gaps and the grapevine varieties planted on each zone. Mapping grapevine resource yield-gaps are the first stage for understanding grapevine yield-gaps at the regional scale.

Efecto de distintos ambientes sobre las características físico – químicas y sensoriales del Montepulciano d’Abruzzo DOC

La región de Abruzzo está situada entre los Apeninos y el mar Adriático, limitando al norte con el río Tronto y al sur con el Trigno. Desde un punto de vista físico se divide en dos franjas

EXTRACTIBLE COMPOUNDS FROM MICROAGGLOMERATED CORK STOPPERS

After bottling, the wine continues to evolve during storage. The choice of the stopper is an important factor in this evolution. In addition to the oxygen permeability of the closure, the migration of stopper compounds into the wine can also have an impact on the wine organoleptic properties. Many studies have shown that transfers of volatile compounds from the stoppers into the wine can happen depending on the type of closure used (1). Moreover, when cork-made stoppers are used, the migration of phenolic compounds from the stopper into the wine can also occur (2, 3).

OTA DEGRADATION BY BACTERIAL LACCASEST

Laccases from lactic acid bacteria (LAB) are described as multicopper oxidase enzymes with copper union sites. Among their applications, phenolic compounds’ oxidation and biogenic amines’ degradation, have been described. Besides, the role of LAB in the toxicity reduction of ochratoxin A (OTA) has been reported (Fuchs et al., 2008; Luz et al., 2018). Fungal laccases, but not bacterial laccases, have been screened for OTA and mycotoxins’ degradation (Loi et al., 2018). OTA is a mycotoxin produced by some fungal species, such as Penicillium and Aspergillus sp., which infect grape bunches used for winemaking.

Three proximal sensors to estimate texture, skeleton and soil water storage in vineyards

Proximal sensors are becoming widely used in precision viticulture, due to the quick, easy and non-invasive identification of soil spatial variability. The apparent soil electrical conductivity (ECa) is the main parameter measured by sensors, which is correlated to many factors, like soil water content, salinity, clay content and mineralogy, rock fragments, bulk density, and porosity.