Exploring the gene regulatory networks of WRKY family in grapevine (Vitis vinifera  L.) using DAP-Seq

Aim: Wine aromatic profile is a combination of viticulture and oenological practices and it is related to character, quality, and consumer acceptance. Based on the competition between soil capacity and canopy development, and on the potential to produce sparkling wines at Caldas, in the south region of Minas Gerais (Brazil) (21°55´S and 46°23´W, altitude 1.100m), the aim of this work was the evaluation of the development of aromas (secondary metabolites) from grapes to sparkling wines in a vine-spacing experiment and whether the distance between the vines can influence the aromatic profile of the sparkling wines (final product). 

“Vinum debet esse naturale ex genimine vitis et non corruptum”. The Eucharistic wine must be made with pure grapes that must not be contaminated in any way. This is how wine was born in the monastery of the Augustinians, and that is how the genetic improvement of grapevine implemented over the decades at the Agricultural Institute of San Michele all’Adige (since 1874; Trentino – Italy) has been oriented to make the cultivation of grapes always more sustainable. This concept is still current and meets the worldwide urgent need of reducing the use of chemicals, under a climate crisis scenario. Since the beginning of the twentieth century, the varieties introduced in Trentino and the new cultivars produced by pioneer breeders have already embraced the principle of sustainable viticulture.

In the context of increasingly hot and dry summers, the adoption of innovative irrigation technologies has become essential for maintaining grape production while minimizing water use.

A consequence of climate change is the modification of grape harvest quality and physico-chemical parameters of the obtained wine: increase in alcoholic degree, decrease in pH, and modification of the extractability of macromolecules, which leads to problems of microbiological, tartaric, colour and colloidal stability. In order to respond to these problems, the winemaking processes must be anticipated and adapted with a better knowledge of macromolecule extractability in grapes and their evolution, according to the grape variety, vintage and winemaking process. The purpose of this study was to understand 1) how the harvest date can influence the extractability of macromolecules, polysaccharides and phenolic compounds, which are responsible for wine stability 2) how to adapt the winemaking process to the harvest date in order to optimise wine quality.

The under-way zoning works of the Emilia viticulture have pointed out a huge variability of the features of the soils, which belong to this area.