terclim by ICS banner
IVES 9 IVES Conference Series 9 Exploring zoxamide sensitivity in Plasmopara viticola populations: implications for fungicide management in precision agriculture

Exploring zoxamide sensitivity in Plasmopara viticola populations: implications for fungicide management in precision agriculture

Abstract

Fungicides play a critical role in managing grapevine downy mildew caused by the oomycete Plasmopara viticola, a biotrophic and polycyclic pathogen with a high risk of fungicide resistance. Zoxamide, categorized as a low to medium resistance risk, disrupts cell division by inhibiting tubulin polymerization. Resistance to zoxamide is uncommon in field isolates. This six-year study (2017-2022) aimed to detect and quantify zoxamide sensitivity in P. viticola populations across varying resistance pressures in Italian grapevine regions. Analysis of 126 samples from 57 vineyards, mainly in North-Eastern Italy, revealed that most samples exhibited EC50, EC95, and MIC values below 0.1 and 10 mg/L of zoxamide, respectively. Nineteen vineyards showed reduced sensitivity (MIC>100 mg/L), but only four samples were characterized by 24-54% resistant oospores at >100 mg/L of zoxamide. Notably, samples treated 4-5 times displayed a broader distribution of toxicological parameters, suggesting a heightened need to manage fungicide applications to reduce selection pressure. In conclusion, oospore assays proved valuable not only for detecting the overall sensitivity profile of populations but also for quantifying resistant individuals within them, enabling a better identification of critical factors affecting zoxamide sensitivity and highlighting the need for improved management practices in a precision agriculture context.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Mattia Peracchi1*, Beatrice Lecchi1, Giuliana Maddalena1, Silvia Laura Toffolatti1

1 Università degli Studi di Milano, Dipartimento di Scienze Agrarie e Ambientali – DISAA, Via Celoria 2, 20133 Milano2

Contact the author*

Keywords

plant disease, integrated pest management, disease control, fungicide resistance

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Great highlands wine growing terroir: conditions and expressions

During 1982 started our wine growing project at the Puntalarga Hill, between 2500 and 2600 meters a.s.l.: 5.78 ºN, 72.98 ºW. Pinot noir, white Riesling and Riesling x Silvaner crossings are the most planted grapevines. Since 1984 research and development activities are carried out on pertinent subjects.

Photoselective shade films affect grapevine berry secondary metabolism and wine composition

Grapevine physiology and production are challenged by forecasted increases in temperature and water deficits. Within this scenario, photoselective overhead shade films are promising tools in warm viticulture areas to overcome climate change related factors. The aim of this study was to evaluate the vulnerability of ‘Cabernet Sauvignon’ grape berry to solar radiation overexposure and optimize shade film use for berry integrity. A randomized complete block design field study was conducted across two years (2020-2021) in Oakville, Napa Valley, CA, with four shade films (D1, D3, D4, D5) differing in the percent of radiation spectra transmitted and compared to an uncovered control (C0). Integrals for gas exchange parameters and mid-day stem water potential were unaffected by the shade films in 2020 and 2021. By harvest, berries from uncovered and shaded vines did not differ in their size or primary metabolism in either year. Despite precipitation exclusion during the dormant season in the shaded treatments, yield did not differ between them and the control in either season. In 2020, total skin anthocyanins (mg/g fresh mass) in the shaded treatments was greater than C0 during berry ripening and at harvest. Conversely, flavonol concentrations in 2020 were reduced in shaded vines compared to C0. The 2020 growing season highlighted the impact of heat degradation on flavonoids. Flavonoid concentrations in 2021 increased until harvest while flavonoid degradation was apparent from veraison to harvest in 2020 across shaded and control vines. Wine analyses highlighted the importance of light spectra to modify wine composition. Wine color intensity, tonality and anthocyanin values were enhanced in D4 whereas antioxidant properties were enhanced in C0 and D5 wines. Altogether, our results highlighted the need of new approaches in warm viticulture areas given the impact that composition of light has on berry and wine quality.

Management of grapevine water status with the DSS Vintel® provides evidence of sustainable irrigation strategies while maintaining wine quality of Pinot gris in Friuli-Venezia Giulia region, NE italy

Deficit irrigation strategies can be valuable means to improve grape quality while saving important amounts of water. A simple way to use deficit irrigation can be based on irrigating a vineyard with a determined level of crop evapotranspiration. Using a precise physiological parameter indicating water status, irrigation could be managed to maintain a specific pre-dawn leaf water potential.

Application of GiESCO “bio-metaethics” charter in practice: the “direct” involvement of vine grower, winemaker, society

On the basis of a direct agreement between the GiESCO and the vine grower, the winemaker and the consumers (individual; company; public or private organizations), the communication on the content of the charter can be done as follows:
• Commitment to respect the basic rules of the GiESCO “BIO – MetaEthics” charter.
1/ Put Mankind in the depth of all concerns in a universal context: (grower, consumer, citizen, work valuing, education, security)
2/ Insure minimum impact on environment by optimizing cultivation technics: (maximum of natural biodegradable products, friendly practices, short channels, renewable energies, terroir sustainability)

The history of the first demarkated wine region of the world – the Tokaj wine region

The optimal climatic conditions of the region were proved in 1867, when a leaf-print of Vitis tokaiensis was found in a stone from miocen age (13 million years ago).