OENO IVAS 2019 banner
IVES 9 IVES Conference Series 9 Research on the origin and the side effects of chitosan stabilizing properties in wine

Research on the origin and the side effects of chitosan stabilizing properties in wine

Abstract

Fungal chitosan is a polysaccharide made up of glucosamine and N-acetyl-glucosamine and derived from chitin-glucan of Aspergillus niger or Agaricus bisporus. Fungal chitosan has been authorized as an antiseptic agent in wine since 2009 (OIV) and in organic wine in 2018. At the maximum dose of 10g/hl, it was shown to eliminate Brettanomyces bruxellensis, the main spoilage agent in red wines. Fungal chitosan is highly renewable, biocompatible (ADI equivalent to sucrose) and non-allergenic. However, winemakers often prefer to use sulfites (SO2), though sulfites are classified as priority food allergens, than chitosan. Indeed, many conflicting reports exist regarding its efficiency and its side effects towards beneficial wine microorganisms or wine taste. These contradictions could be explained by the heterogeneity of the fungal chitosan lots traded, the diversity of the wines (chemical composition, winemaking process), but also, by the recently highlighted huge genetic diversity prevailing in wine microbial species. 

The CHITOWINE project (ANR 17-CE21-0006) is based on the collaboration of three academic partners, a technology transfer unit and an industrial partner. It primarily aims to better define the potential and limitations of fungal chitosan use as an antimicrobial agent in wine. The work will first enable to better define the spectrum of fungal chitosan through the screening of a large microbial collection representative of the inter- and intra-specific diversity of wine ecosystem (more than 200 strains in 17 species of yeasts and bacteria). The chemical characteristics essential to the antiseptic activity of fungal chitosan (degree of acetylation, molecular weight, solubility and charge) and the influence of extrinsic parameters of reaction (pH, temperature, and dose) will be also evaluated. In addition, the physiological effects of chitosan will be sought through biochemical, microscopic and transcriptomic tests, to identify, if possible, the molecular targets of chitosan and to understand the sensitivity differences observed, between inter or intra species and between strains in the same species. Based on these results, improved use recommendation will be proposed and evaluated. Analytical methods to guide chitosan use will be developed and optimized.

DOI:

Publication date: June 23, 2020

Issue: OENO IVAS 2019

Type: Article

Authors

Marguerite Dols-Lafargue, Margot Paulin, Cécile Miot-Sertier, olivier Claisse, Patricia Ballestra, Warren Albertin-Leguay, Isabelle Masneuf Pomarède, Axel Marchal, Clément Brasselet, Cédric Delattre, Guillaume Pierre, Pascal Dubessay, Christine Gardarin, Philippe Michaud, Thierry Doco, Joana Coulon, Arnaud Massot, Lucie Dutilh, Amélie Vallet-Courbin, Julie Maupeu

Unité de recherche Oenologie, EA 4577, USC 1366 INRA, ISVV, Université de Bordeaux, Bordeaux INP, F33882 Villenave d’Ornon France 

Contact the author

Keywords

chitosan, antiseptic, efficiency, side-effects 

Tags

IVES Conference Series | OENO IVAS 2019

Citation

Related articles…

Soil carbon changes and greenhouse gas emissions in vineyards – Is the 4 per 1000 goal realistic?

In this video recording of the IVES science meeting 2023, Hans Reiner Schultz (Hochschule Geisenheim University, Germany) speaks about soil carbon changes and greenhouse gas emissions in vineyards – is the 4 per 1000 goal realistic?. This presentation is based on an original article accessible for free on OENO One.

Saccharomyces cerevisiae – Oenococcus oeni – Lactiplantibacillus plantarum: focus on malolactic fermentation during production of Catarratto and Riesling white wines

The increasing interest in enhancing groundbreaking sensory profile of wines determined the need to select novel strains of lactic acid bacteria (LAB). Metabolic processes characterizing malolactic fermentation (MLF) lead to the production of several organic compounds that significantly impact the oenological and sensory characteristics of wines.

New oenological technology for adaptation to climate change: reduction of alcohol content during wine fermentation through stripping, with fermentative CO2

The capture and valorization of fermentative CO2 have been developed for several years by the company w platform for internal uses, notably in the cellars: inerting, cooling, reduction of water consumption, extraction, with aroma valorization. In a context of climatic warming during the vegetative cycle, grapes are currently harvested with a significant sugar concentration, a phenomenon that is expected to intensify in the coming decades. The high alcohol content of the resulting wines goes against the demand of customers who are seeking high-quality wines with less alcohol.

Rapid damage assessment and grapevine recovery after fire

There is increasing scientific consensus that climate changeis the underlying cause of the prolonged dry and hot conditions that have increased the risk of extreme fire weather in many countries around the world. In December 2019, a bushfire event occurred in the Adelaide Hills, South Australia where 25,000 hectares were burnt and in vineyards and surrounding areas various degrees of scorching and infrastructure damage occurred. The ability to coordinate and plan recovery after a fire event relies on robust and timely data. The current practice for measuring the scale and distribution of fire damage is to walk or drive the vineyard and score individual vines based on visual observation. The process is time consuming, subjective, or semi-quantitative at best. After the December 2019 fires, it took many months to access properties and estimate the area of vineyard damaged. This study compares the rapid assessment and mapping of fire damage using high-resolution satellite imagery with more traditional ground based measures. Satellite imagery tracking vineyard recovery in the season following the bushfire is being correlated to field assessments of vineyard productivity such as canopy health and development, fertility and carbohydrate storage. Canopy health in the seasons following the fires correlated to the severity of the initial fire damage. Severely damaged vines had reduced canopy growth, were infertile or had very low fertility as well as lower carbohydrate levels in buds and canes during dormancy, which reduced productivity in the seasons following the bushfire event. In contrast, vines that received minor damage were able to recover within 1-2 years. Tools that rapidly and affordably capture the extent and severity of damage over large vineyard area will allow producers, government and industry bodies to manage decisions in relation to fire recovery planning, coordination and delivery, improving the efficiency and effectiveness of their response.

Changes in white wine composition after treatment with cationic exchange resin: impact on wine oxidation after 8 years of bottle storage

Samples from 3 wine types were treated with a cationic exchange resin (7 lots) and stored for 8 years (47 samples). Forty-seven parameters were determined, including (1) important substrates with impact in white wine oxidation and (2) markers of oxidation. From group 1, sugars, elements, phenolic compounds, α-dicarbonyls and SO2 and from group 2, browning (A420), acetaldehyde, alkanals, furanic compounds were quantified.