Crossed approaches to experimental economics and sensory analysis regarding noble rot sweet wines perception

Challenges associated with climate change, such as soil erosion and drought, have impacted viticulture across wine regions globally in recent decades. As winegrowers struggle to maintain yield and quality standards under these conditions, methods to adapt to and mitigate the impacts of climate change have become more prevalent. One potential mitigation strategy is to enhance symbiotic interaction of grapevine roots with arbuscular mycorrhizal fungi (AMF).

The objective of the present work is to investigate wine produced from dehydrated grapes and vinified according to classical Roman manuals.

METHODS – Locally produced Muscat of Alexandria’s grapes were used for the sweet wine production, grown in the experimental vineyard of Instituto Superior de Agronomia (Lisbon, Portugal). The grapes were harvested manually slightly over-ripe and subjected to greenhouse drying. After 7-10 days dried grapes were transported to an experimental winery for various operations (e.g., grape weighing, sorting, crushing/destemming). Several maceration protocols were used comprising the addition of saltwater and white wine to whole bunches or destemmed grapes. Fermentation was conducted with the addition of commercial yeast. The standard physico-chemical parameters of wines were determined according to the OIV standards.

Context and purpose of the study. Daylength is a key climatic factor within the terroir concept. However, the complex interplay of multiple variables in regions with varying daylengths makes it challenging to isolate and investigate this specific factor.

Downy mildew, a disease caused by Plasmopara viticola (Berk. et Curt.) Berl. and De Toni, is one of the strongest threats to grapevine (Vitis vinifera L.) production. Recently, sources of resistance to downy mildew were identified among Caucasian germplasm. Among them, the Georgian variety Mgaloblishvili revealed a unique resistance mechanism. A genome wide association study (GWAS) allowed the identification of the genetic bases of Mgaloblishvili resistance, the loci Rpv29, Rpv30 and Rpv31. To dissect the three resistance loci, Mgaloblishvili genome was sequenced using PacBio HiFi reads and assembled.

New Plant Breeding Techniques (NPBTs) have arisen with the objective of surmounting the constraints inherent in conventional breeding methodologies, thereby enhancing plant resilience against both biotic and abiotic stresses. To date the application of genome editing in grapevine is still limited by the necessity to overcome recalcitrance to produce embryogenic calli and to regenerate plants. In our studies, we developed a smart and versatile genetic transformation system carrying all the most promising features of different genome editing approaches. In specific, we joined the GRF-GIF expression to improve regeneration, the systemic movement of the editing transcripts through tRNA-like sequences (TLS) and the cisgenic-like approach to remove transgenes.