Isolation of indigenous yeast strains from the Purcari and Trifești wine centers in the Republic of Moldova and evaluation of their impact on the quality of dry red wines
Abstract
In the conducted research, 30 yeast strains from red grape varieties were isolated from the Purcari wine center, and 28 yeast strains from red grape varieties were isolated from the Trifești wine center in the Republic of Moldova. Morphological and cultural analysis revealed their diversity, confirming that all strains belong to the class Ascomycotina. Nucleotide sequencing comparisons were made with sequences deposited in the NCBI genetic bank (ncbi.nlm.nih.gov), and the yeasts were identified using the FT-IR method. Based on the biochemical and technological indices of the isolated yeast strains, the following strains were selected: from the Purcari wine center – No.21 – C-S-120-P-2, No.24 – R-NNP-2, No.29 – R-N-120-P-4, No.30 – R-N-120-P-5; from the Trifești wine center – No.27 – MTr-4, No.32 – M100Tr-1, No.35 – M100Tr-4, No.41 – C-S60Tr-2, No.43 – C-S60Tr-4. In micro-vinification conditions, it was established that the use of selected yeast strains from the Purcari wine center (No.30 – R-N-120-P-5) and Trifești wine center (No.32 – M100Tr-1, No.41 – C-S60Tr-2) allows the production of high-quality dry red wines, both by physicochemical indices and organoleptic ratings, and does not compromise the quality of wines made with imported Dry Active Yeasts.The selected indigenous yeast strains were deposited in the National Collection of Non-Pathogenic Microorganisms, and storage certificates and passports were obtained for each yeast strain, with the identification code assigned by the National Collection of Non-Pathogenic Microorganisms of the Institute of Microbiology and Biotechnology, Technical University of Moldova.
DOI:
Publication date: 22/09/2025
Issue: 46th World Congress of Vine and Wine
Type: Short communication
Authors
1 PI National Institute for Applied Research in Agriculture and Veterinary Medicine, Chishinau, MD-2070, Republic of Moldova