Terroir 2020 banner
IVES 9 IVES Conference Series 9 Regional discrimination of shiraz using targeted and non-targeted analytical approaches

Regional discrimination of shiraz using targeted and non-targeted analytical approaches

Abstract

Aims: Shiraz is the most widely cultivated grape variety in Australia, and is grown under a range of viticultural and climatic conditions. Given its importance to the Australian wine sector, a number of studies have been conducted in recent years which involved a comprehensive assessment of grape composition, in order to objectively predict wine quality and style outcomes. It was of interest to reanalyse this compositional database to determine if regional variation in Shiraz composition exists, and if so, to identify analytical approaches which might best discriminate the response of this variety to the unique growing conditions imposed by regional or sub-regional variables. 

Methods and Results: For a preliminary regional study, Shiraz grapes were obtained from multiple geographical indices within South Australia, and analysed for a range of targeted volatile and non-volatile compounds, as well as by non-targeted near- and mid-infrared approaches. Using multivariate modelling, it was found that data generated using both the targeted and non-targeted analytical approaches could discriminate the samples on a regional basis. For a focused study on site diversity within the Barossa Valley, Shiraz grape samples were collected from a number of sub-regions, and from multiple locations within each vineyard (5-10). Grapes were micro-vinified, and grape and wine samples were further analysed for non-volatiles using targeted and non-targeted approaches. Grape samples were also assessed using near- and mid-infrared spectroscopy. It was found using the targeted analytical approach that within-vineyard variability exceeded between-vineyard variation for some measures, preventing discrimination of vineyards or sub-regions using multivariate modelling. However, using the data generated from multiple non-targeted analytical approaches, within-vineyard variation was substantially reduced. This enabled Shiraz vineyards to be clearly defined using a non-targeted ‘chemical fingerprint’ and showed some potential to discriminate the Barossa sub-regions. Mass spectra generated using the non-targeted profiling approach were further assessed, and enabled the identification of grape-derived compounds which were relevant to the sub-regional response. 

Conclusion:

Non-targeted profiling of grapes and wines showed the potential to discriminate geographical indices (region) as well as sites within a region, even though absolute differences in grape composition could be substantial. This indicates that certain aspects of grape chemistry are more sensitive to site- or region-specific variables than others. Further work could seek to identify individual compounds, or classes of compounds, which most consistently define the ‘terroir’ response for the Shiraz grape variety. 

Significance and Impact of the Study: Using the results of this study, new methods could be developed to quantify the relevant grape or wine metabolites identified using the non-targeted approach, in order to apply these more broadly within studies which seek to objectively characterise ‘terroir’.

DOI:

Publication date: March 16, 2021

Issue: Terroir 2020

Type: Video

Authors

Keren Bindon1*, Paul Smith1,2, Dylan Grigg3, Natoiya Lloyd1, Luca Nicolotti1, Jean Macintyre4, Roberta De Bei3, Cassandra Collins3

1The Australian Wine Research Institute, PO Box 197, Glen Osmond, SA 5064, Australia
2Wine Australia, Industry House-National Wine Centre, Cnr Hackney and Botanic Roads, SA 5000, Australia
3The University of Adelaide, PMB 1, Glen Osmond, SA, 5064, Australia
4Pernod Ricard Winemakers, 1914 Barossa Valley Way, Rowland Flat, SA, 5352, Australia

Contact the author

Keywords

Shiraz, objective measures, grape and wine quality

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Long-term drought resilience of traditional red grapevine varieties from a semi-arid region

In recent decades, the scarcity of water resources in agriculture in certain areas has been aggravated by climate change, which has caused an increase in temperatures, changes in rainfall patterns, as well as an increase in the frequency of extreme phenomena such as droughts and heat waves. Although the vine is considered a drought-tolerant specie, it has to satisfy important water requirements to complete its cycle, which coincides with the hottest and driest months. Achieving sustainable viticulture in this scenario requires high levels of efficiency in the use of water, a scarce resource whose use is expected to be severely restricted in the near future. In this regard, the use of drought-tolerant varieties that are able to maintain grape yield and quality could be an effective strategy to face this change. During three consecutive seasons (2018-2020) the behavior in rainfed regime of 13 traditional red grapevine varieties of the Spain central region was studied. These varieties were cultivated in a collection at Centro de Investigación de la Vid y el Vino de Castilla-La Mancha (IVICAM-IRIAF) located in Tomelloso (Castilla-La Mancha, Spain). Yield components (yield, mean bunch and berry weight, pruning weight), physicochemical parameters of the musts (brix degree, total acidity, pH) and some physiological parameters related with water stress during ripening period (δ13C, δ18O) were analysed. The application of different statistical techniques to the results showed the existence of significant differences between varieties in their response to stressful conditions. A few varieties highlighted for their high ability to adapt to drought, being able to maintain high yields due to their efficiency in the use of water. In addition, it was possible quantify to what extent climate can be a determinant in the δ18O of musts under severe water stress conditions.

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

Effect of fertigation strategies to adapt PGI Côtes de Gascogne production to hot vintage

The development of fertigation could be a possible solution to adapt PGI Côtes de Gascogne (south-western France) wine production to climate change. The goal would be to limit the negative effects of water stress on yield performance expectation (around 15 tons per hectare) and to make the use of fertilizers more efficient. This study aimed to compare the effects of three strategies of water and minerals supply on grapes and wines qualities. Two fertigation practices were compared to a rainfed control which is the current standard of the local grape growing production. The fertilizers (nitrogen and potassium) were (i) fully brought by irrigation pipe during the season, (ii) partially brought by irrigation pipe and partially on the soil or (iii) fully brought on the soil at the beginning of the season for the non-irrigated control (local standard). The trial was run on cv. Colombard trained on spur pruned with vertical shoot positioning system on a sandy-silty-clay soil over the 2020 vintage which was particularly hot for the region. Moderate to strong water deficit appeared during the growing period of the berries and held on after veraison. Irrigation strategies allowed for maintaining grapevine without water deficit and being significantly different from the control water status. Grapevine with fully or partial fertigation strategies produced 25% more yield mainly due to the increase of the bunch weight. Also, the fully fertigation showed the best ratio between yield and maturity and brought 30% less of fertilizers (both nitrogen and potassium) than the two other strategies. Finally, the analysis of aromatic compounds in Colombard wines, varietal thiols family, showed the same level of concentrations for the 3 treatments, confirming that the yield performance did not impact the aromatic potential in this trial.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...

Spatial variability of temperature is linked to grape composition variability in the Saint-Emilion winegrowing area

Elevated temperature during the grape maturation period is a major threat for grape quality and thus wine quality. Therefore, characterizing the grape composition response to temperature at a larger scale would represent a crucial step towards adaptation to climate change. In response to changes in temperature, various physiological mechanisms regulate grape composition. Primary and secondary metabolisms are both involved in this response, with well-known effects, for example on anthocyanins, and lesser known effects, for example on aromas or aroma precursors. At the field scale or at the regional scale, however, numerous environmental or plant-specific factors intervene to make the effects of temperature difficult to distinguish from overall variability. In this study, it was attempted to overcome this difficulty by selecting well-characterized situations with differing temperatures.
A long-term study of air temperature variability across several Merlot vineyards in the Saint-Emilion and Pomerol wine producing area found significant temperature differences and gradients at various time scales linked to environmental factors. From this study area, a few sites were selected with similar age, soil and training system conditions, and with repeated and contrasted temperature differences during the maturation period. The average temperature difference during the maturation period was about 2°C between cooler and warmer sites, a difference similar to that expected under future climate change scenarios. In close vicinity to the temperature sensors at each site, grape berries were sampled at different times until full maturity during 2019 and 2020. Also, berries from bunches on either side of the row were analyzed separately, allowing an investigation of bunch exposure effect associated with the coupling of berry temperature and solar radiation. Four replicates of pooled berries for each time – site – bunch exposure combination were obtained and analyzed for biochemical composition. Analyses of variance of the biochemical composition data collected at different sampling times reveal significant effects associated with temperature, site, and bunch azimuth. For instance, anthocyanins in grape skins are clearly influenced by temperature and solar radiation exposure, with up to 30% reduction in warmer conditions.