Terroir 2020 banner
IVES 9 IVES Conference Series 9 Juice carbon isotope discrimination is related to vine growth and fruit quality of Barossa Shiraz

Juice carbon isotope discrimination is related to vine growth and fruit quality of Barossa Shiraz

Abstract

Aim: Interactions between soil, climate and management that modulate vine growth, yield and grape composition are strongly defined by vine water availability and nutrient uptake during the season. Carbon isotope discrimination (δ13C) has been used as an integrative measurement of vine water availability during the season, with the potential to identify spatial variations of terroir in vineyards that do not receive irrigation. We measured juice δ13C at harvest across multiple vineyards with the aim to discriminate sub-regions based on soil water availability due to variation in climate, soil and management (especially supplementary irrigation). We explored the relationship between δ13C and pruning mass, yield and fruit parameters important for wine quality.

Methods and Results: The study was conducted in 2019 in irrigated Shiraz vineyards spread across six sub-regions in the Barossa Valley, SA. A total of 63 samples collected at harvest (approx. 25⁰ Brix) were subject to δ13C analysis, this included three samples from each of 21 vineyards. Yield, pruning mass and berry maturity (total soluble solids, titratable acidity and pH) and quality parameters (total tannins, anthocyanins and phenolics) were assessed. Carbon isotope composition of the grape sugars was measured on autoclaved berry juice using a continuous flow isotope ratio mass spectrometer. δ13C discriminated between sub-regions and within vineyards. Vineyards from sub-regions, Eden Valley, Central and Northern Grounds had lower δ13C than vineyards from the Western Ridge and Eastern Edge, with the Southern Grounds. Similarly, zones within a vineyard with lower plant biomass, as indicated by PCD imagery, showed lower δ13C. A significant relationship was observed between δ13C and yield (r = -0.72***), pruning mass (r = -0.54**), anthocyanins (r = 0.65**) and total phenolics (r = 0.61**). Higher water stress (< δ13C) during the season was associated with a lower yield, lower pruning mass but with higher total anthocyanins and phenolics. No significant relationships between δ13C and other berry traits (including total tannins) were observed.

Conclusions: 

δ13C is a useful method to integrate and distinguish components of terroir that affect vine productivity and some fruit quality parameters which remains sound even when the vines receive irrigation. 

Significance and Impact of the Study: This study shows the potential use of δ13C to discriminate between blocks with different moisture availability that may induce changes in yield and some aspects of fruit quality. δ13C may emerge as a proxy for terroir in zoning studies of irrigated vines, but further validation is needed using cluster analysis that integrates soil, climate and fruit composition geospatially across multiple seasons.

DOI:

Publication date: March 17, 2021

Issue: Terroir 2020

Type: Video

Authors

Marcos Bonada1, Cassandra Collins2, Paul Petrie1

1South Australian Research and Development Institute, Urrbrae, Australia 2The University of Adelaide, School of Agriculture, Food and Wine, Waite Research Institute, Glen Osmond, Australia

Keywords

Carbon isotope discrimination, water availability, grapevine growth, fruit composition, terroir

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

The modification of cultural practices in grapevine cv. Syrah, does it modify the characteristics of the musts?

The work shows the results of a year of experimentation (2020) in a Syrah variety vineyard in La Roda (Castilla-La Mancha, Spain). The trial approach was on a randomized block design with two factors: Irrigation (I) and Pruning (P).
Irrigation schedules were adjusted to apply amounts close to 1,500 m3/ha. With this provision, 2 different irrigation treatments were proposed: I1) Start of irrigation from pea-sized grape to post-harvest (providing at least 20 % of the total amount of irrigation water to be provided post-harvest); I2) Start of irrigation from pea-sized grape to harvest (usual irrigation practice in the study area). Pruning was proposed with two treatments, one at the end of January (P1), which is pruning on a conventional date; and P2) pruning carried out at the beginning of budding. In total, 4 repetitions were designed with 4 elementary plots, each one of them representing one of the proposed treatments (I1P1; I1P2; I2P1; I2P2). In total, 16 plots were worked on and each elementary plot consisted of 30 strains, distributed in 3 lines.
The productive response was evaluated with the yield results of the harvest harvested at 23 ºBrix. The qualitative response was measured in the musts through the indices of technological (acidity, pH and potassium) and phenolic maturity and aromatic compounds in free and glycosylated fractions. The treatments tested had, in general, an effect on the different variables analyzed.

Analysis of some environmental factors and cultural practices that affect the production and quality of the Manto Negro, Callet and Prensal Blanc varieties

45 non irrigated vineyards distributed in the DO (Denomination) Pla i Llevant de Mallorca and the DO Binissalem Mallorca were used to investigate the characteristics of production and quality and their relationships certain environmental factors and cultural practices. The grape varieties investigated are autochthonous to the island of Mallorca, Manto Negro and Callet as red and Prensal Blanc as white. All plants were measured for four consecutive years in the main production and quality parameters. Among the environmental factors, the type of soil has been studied, more specifically its water retention capacity, the planting density, the age of the vineyard and the level of viral infection. The presence or absence of virus seems to have no effect on any component studied in the varieties studied. For the white variety Prensal Blanc age is negatively correlated with production and the number of bunches, nevertheless it does not cause any effect on the required quality parameters. However, for the red varieties Callet and Manto Negro, the age of the plantation is the variable that best correlates with the quality parameters, therefore the old vines should be the object of preservation by the viticulturists and winemakers in order to guarantee its contribution to the quality of the wines made with these varieties.

Grapevine yield-gap: identification of environmental limitations by soil and climate zoning in Languedoc-Roussillon region (south of France)

Grapevine yield has been historically overlooked, assuming a strong trade-off between grape yield and wine quality. At present, menaced by climate change, many vineyards in Southern France are far from the quality label threshold, becoming grapevine yield-gaps a major subject of concern. Although yield-gaps are well studied in arable crops, we know very little about grapevine yield-gaps. In the present study, we analysed the environmental component of grapevine yield-gaps linked to climate and soil resources in the Languedoc Roussillon. We used SAFRAN data and IGP Pays d’Oc wine yields from 2010 to 2018. We selected climate and soil indicators proving to have a significant effect on average wine yield-gaps at the municipality scale. The most significant factors of grapevine yield were the Soil Available Water Capacity; followed by the Huglin Index and the Climatic Dryness Index. The Days of Frost; the Soil pH; and the Very Hot Days were also significant. Then, we clustered geographical zones presenting similar indicators, facilitating the identification of resources yield-gaps. We discussed the number of zones with the experts of IGP Pays d’Oc label, obtaining 7 zones with similar limitations for grapevine yield. Finally, we analysed the main resources causing yield-gaps and the grapevine varieties planted on each zone. Mapping grapevine resource yield-gaps are the first stage for understanding grapevine yield-gaps at the regional scale.

Rootstock regulation of scion phenotypes: the relationship between rootstock parentage and petiole mineral concentration

Grapevine is grown as a graft since the end of the 19th century. Rootstocks not only provide tolerance to Phylloxera but also ensure the supply of water and mineral nutrients to the scion. Rootstocks are an important mean of adaptation to environmental conditions, because the scion controls the typical features of the grapes and wine. However, among the large diversity of rootstocks worldwide, few of them are commercially used in the vineyard. The aim of this study was to investigate the extent to which rootstocks modify the mineral composition of the petioles of the scion. Vitis vinifera cvs. Cabernet-Sauvignon, Pinot noir, Syrah and Ugni blanc were grafted onto 55 different rootstock genotypes and planted in a vineyard as three replicates of 5 vines. Petioles were collected in the cluster zone with 6 replicates per combination. Petiolar concentrations of 13 mineral elements (N, P, K, S, Mg, Ca, Na, B, Zn, Mn, Fe, Cu, Al) at veraison were determined. Scion, rootstock and the interaction explained the same proportion of the phenotypic variance for most mineral elements. Rootstock genotype showed a significant influence on the petiole mineral element composition. Rootstock effect explained from 7 % for Cu to 25 % for S of the variance. The difference of rootstock conferred mineral status is discussed in relation to vigor and fertility. Rootstocks were also genotyped with 23 microsatellite markers. Data were analysed according to genetic groups in order to determine whether the petiole mineral composition could be related to the genetic parentage of the rootstock. Thanks to a highly powerful design, it is the first time that such a large panel of rootstocks grafted with 4 scions has been studied. These results give the opportunity to better characterize the rootstocks and to enlarge the diversity used in the vineyard.

Is wine terroir a valid concept under a changing climate?

The OIV[i] defines terroir as a concept referring to an area in which collective knowledge of the interactions between the physical and biological environment (soil, topography, climate, landscape characteristics and biodiversity features) and vitivinicultural practices develops, providing distinctive wine characteristics. Those are perceptible in the taste of wine, which drives consumer preference and, therefore, wine’s value in the marketplace. Geographical indications (GI) are recognized regulatory constructs formalizing and protecting the nexus between wine taste and the terroir generating it. Despite considering updates, GIs do not consider the nexus as a dynamic one and do not anticipate change, namely of climate. Being climate a fundamental feature of terroir, it strongly impacts wine characteristics, such as taste. According to IPCC[ii], many widespread, rapid and unprecedented changes of climate occurred, some being irreversible over hundreds to thousands of years. Climatic shifts and atmospheric-driven extreme events have been widely reported worldwide. Recent climatic trends are projected to strengthen in upcoming decades, whereas extremes are expected to increase in frequency and intensity, forcing wines away from GI definitions. Geographical shifts of viticultural suitability are projected, often moving into regions and countries different from current ones. Some authors propose adaptation in viticulture, winemaking and product innovation. We show evidence of climate changing wine characteristics in the Douro valley, home of 270-year-old Port GI. We discuss herein resist or adapt stances for when climate changes the nexus between terroir and wine characteristics. Using the MED-GOLD[iii] dashboard, a tool allowing for easy visual navigation of past and future climates, we demonstrate how policymakers can identify future moments, throughout the 21st century under different emission scenarios, when GI specifications will likely need updates (e.g., boundaries, varieties) to reduce climate-change impacts.