Terroir 2020 banner
IVES 9 IVES Conference Series 9 International Terroir Conferences 9 Terroir 2020 9 History and innovation of terroir 9 How geographical origin and vineyard management influence cv. Cabernet-Sauvignon in Chile – Machine learning based quality prediction

How geographical origin and vineyard management influence cv. Cabernet-Sauvignon in Chile – Machine learning based quality prediction

Abstract

Aims: The aims of this study were to i) characterize the impact of geographical origin and viticulture treatments on Chilean Cabernet-Sauvignon, and ii) develop machine learning models to predict its quality. 

Methods and Results: 100 vineyard plots representing the typical percentage distribution of geographical and viticulture impact factors on Chilean Cabernet-Sauvignon were monitored across two seasons, 2018 and 2019. Chemical analysis of grapes and wines included the quantification of phenolic compounds by liquid chromatography and UV-vis spectral measurements, aroma compounds by gas chromatography mass spectrometry (GC/MS), and maturity parameters. Spearman correlation and Principal component analysis (PCA) identified correlations of several non-volatile and volatile compounds with quality, mainly by means of their anthocyanins, flavonols, flavan‑3‑ols, total tannins and hydroxycinnamic acids. Furthermore by trans-2-hexenol, trans-3-hexenol, hexanal, 2-isobutyl-3-methoxypyrazine (IBMP), yeast assimilable nitrogen (YAN), total soluble solids and acidity. Experimental winemaking of 600 kg per plot followed a standardized procedure, and the wines were analyzed by an expert quality rating. A sensory quality profiling for the wines was performed through a Napping Ultra Flash Profile (UFP). It revealed the distinction of three different quality levels by mainly mouthfeel attributes, and fruity and green aromas. However, neither the observed correlations of chemical analysis and sensory quality ratings, nor origin or viticulture treatment could fully explain quality. Different clustering methods, namely k-means, k-medioids and spectral clustering were evaluated in order to find categories given by the chemical analysis data itself as unsupervised machine learning. Spectral clustering led to optimum results, and independently of sample origin and viticulture traits, quality ratings were characterized to be significantly different across the clusters allowing their interpretation as quality categories. 

Conclusions: 

Chilean Cabernet-Sauvignon quality is associated with chemical quality markers known for this variety in Australia and California, including phenolic compounds, C6 alcohols and aldehydes, IBMP, maturity parameters and YAN. However, evaluation of sensory quality is fairly subjective and viticulture treatments in practical application contain interdependency, therefore it is challenging to establish supervised models involving this data. The application of unsupervised spectral clustering is proposed as an objective quality classification approach, which can be trained using supervised models for predictive purposes.

Significance and Impact of the Study: There is a high industrial need for objective quality classification. For the first time chemical quality markers for Chilean Cabernet-Sauvignon were determined, and an unsupervised machine learning approach based on these markers could be proposed for objective quality classification.

DOI:

Publication date: March 19, 2021

Issue: Terroir 2020

Type: Video

Authors

Doreen Schober1*, Martin Legues1,2, Hugo Guidez3, Jose Carlos Caris Maldonado1, Sebastian Vargas1,  Alvaro Gonzalez Rojas1

1Center for Research and Innovation (CRI), Viña Concha y Toro, Ruta k-650 km 10, Pencahue, Región de Maule, Chile
2Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, Macul, Región Metropolitana, Santiago, Chile
3Institut National Supérieur des Sciences Agronomiques, Agroalimentaires, Horticoles et du Paysage, Agrocampus Ouest Campus d´Angers, France

Contact the author

Keywords

Cabernet-Sauvignon, spectral clustering, quality, terroir, vineyard management

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Late season canopy management practices to reduce sugar loading and improve color profile of Cabernet-Sauvignon grapes and wines in the high irradiance and hot conditions of California Central Valley

Global warming is accelerating grape ripening, leading to unbalanced wines from fruit with high sugar content but poor aroma and colour development. Reducing the size of the photosynthetic apparatus after veraison has been shown to delay technological ripeness in cool climates, but methods have not been tested in areas with high irradiance and temperature where fruit exposure could have disastrous effects on berry composition. In this Cabernet-Sauvignon trial, we compared the application of an antitranspirant (pinolene), to severe canopy topping and above bunch zone leaf removal, all performed at mid-ripening, with an untouched control. We monitored the vines weekly by measuring stem water potential, gas exchange, fruit zone light exposure. We sampled berries to measure berry weight, total soluble solids, pH, titratable acidity, and the anthocyanin profile. At harvest, we assessed yield components, measured carbon isotope discrimination, rated sunburn on clusters, and produced experimental wines. We submitted harvest samples to metabolomic profiling through PFP-Q Exactive MS/MS and wines to sensory analysis. Application of the antitranspirant significantly reduced stomatal conductance and assimilation rate but did not affect the stem water potential. Inversely, leaf removal and topping increased water potential but did not affect leaf gas exchange. The late topping was the only treatment able to decrease sugar content (up to 2Bx), increase titratable acidity and pH, and improve anthocyanin content because of lower degradation of di-hydroxylated forms. Late leaf removal above the bunch zone increased lightning conditions in the canopy and produced the most significant damage on fruits. Yield components were not affected. This work suggests that late-season canopy management can effectively control ripening speeds and improve grapes and wines. Still, the effect on grape exposure in a critical time must be well balanced to avoid problems with the appropriate technique.

Impact of changes in pruning practices on vine growth and yield

A gradual decline in vineyards has been observed over the past twenty years worldwide. This might be explained by the climate change, practices change or the increase of dieback diseases. To increase the longevity of vines, we studied the impact of different pruning strategies in four adult and four young vineyards located in France and Spain. In France, vineyards were planted with Cabernet franc on 3309C while Spanish trials were planted with Tempranillo grafted on 110R. Vegetative expression, yield, quality of berries and wood vessels conductivity were measured. The distribution of vegetative expression, yield and berry composition between primary and secondary vegetation were quantified. Finally, tomography was used to evaluate the implication of the treatments on sap flows.
First results show that i) the respectful pruning leads to an increase of 30 to 50% more secondary shoots than the aggressive pruning in France and between 15 and 20% in Spain, ii) there is no major effect on the yield over the first two years following the implementation of the new pruning practices, although the proportion of clusters from suckers is higher on the respectful pruning method. On young vines, the development of the trunk according to a respectful pruning leads to a loss of harvest 2 years after planting. This is due to the removal, on the future trunk, of the green suckers which carrying bunches. This operation carried out in spring rather than during winter pruning, would promote a better leaf / fruit balance when the plant comes into production, and could lead to better hydraulic conduction in the vessels of the trunk. Maintaining these trials for several years will provide more robust data to assess the impact of these practices on the vines over the long term.

Effects of graft quality on growth and grapevine-water relations

Climate change is challenging viticulture worldwide compromising its sustainability due to warmer temperatures and the increased frequency of extreme events. Grafting Vitis vinifera L.

Towards adaptation to climate change in Rioja: Quality evaluation of wines obtained from Grenache x Tempranillo selections

The wine sector is of great relevance and tradition in Mediterranean countries, however, it may be most susceptible to climate change. In recent years, wine production is facing changes worldwide, both at environmental as well as commercial levels, due to global warming and the shift in consumers’ preferences. Wine growers and wine makers are in search of solutions that allow to face these new challenges. One of the most promising initiatives in the long term is the introduction of new plant materials, specifically intraspecific hybridizations between premium varieties that may improve traditional germplasm in its adaptation to climate change. These inter-varietal crosses have the potential to generate quality wines, whilst maintaining the regional typicity, and constitute an attractive alternative for the consumer due to their sensory attributes. In this study, we have evaluated wines from 29 intraspecific Garnacha x Tempranillo hybrids in two different locations, with the aim to assess their oenological potential and sensory attributes. Thirteen of the selections were white and 16 were red. Microvinifications were conducted with two or three replications depending on grape availability. Conventional oenological parameters were determined for all wines. The sensory evaluation and hedonic scores were given by five experts. Red selections obtained higher quality scores than white ones. Among the white selections with higher quality scores, GT-41 Varea and GT-159 Varea outstand, due to their high total acidity and high malic acid content. Regarding red selections, GT-57 Varea and GT-57 UR were perceived as higher in quality, highlighted for their moderate alcoholic and high anthocyanin content. Our results indicate that intraspecific hybridization may be a powerful tool for adapting traditional cultivars to climate change in Rioja.

The concept of terroir: what place for microbiota?

Microbes play key roles on crop nutrient availability via biogeochemical cycles, rhizosphere interactions with roots as well as on plant growth and health. Recent advances in technologies, such as High Throughput Sequencing Techniques, allowed to gain deeper insight on the structure of bacterial and fungal communities associated with soil, rhizosphere and plant phyllosphere. Over the past 10 years, numerous scientific studies have been carried out on the microbial component of the vineyard. Whether the soil or grape compartments have been taken into account, many studies agree on the evidence of regional delineations of microbial communities, that may contribute to regional wine characteristics and typicity. Some authors proposed the term “microbial terroir” including “yeast terroir” for grapes to describe the connection between microbial biogeography and regional wine characteristics. Many factors are involved in terroir including climate, soil, cultivar and human practices as well as their interactions. Studies considering “microbial terroir” greatly contributed to improve our knowledge on factors that shape the vineyard microbial structure and diversity. However, the potential impact of “microbial terroir” on wine composition has yet not received strong scientific evidence and many questions remain to be addressed, related to the functional characterization of the microbial community and its impact on plant physiology and grape composition, the origins and interannual stability of vineyard microbiota, as well as their impact on wine sensorial attributes. The presentation will give an overview on the role of microbiota as a terroir component and will highlight future perspectives and challenges on this key subject for the wine industry.