Terroir 2020 banner
IVES 9 IVES Conference Series 9 Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum in a “terroir” of La Mancha wine región (Spain)

Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum in a “terroir” of La Mancha wine región (Spain)

Abstract

Aim: Among Botryosphaeriaceae species associated with Botryosphaeria dieback of grapevines, Neofusicoccum parvum is one of the most virulent and fastest wood-colonizing fungi. This study aimed to evaluate the susceptibility of six red grapevine cultivars (“Bobal”, “Monastrell”, “Garnacha Tinta”, “Moravia Agria”, “Tinto Velasco” and “Moribel” to N. parvum, under field conditions.

Methods and Results: Pathogenicity studies were conducted, over two consecutive years, with one-year old grapevine rooted cuttings inoculated with a N. parvum isolate. Rooted cuttings were wounded between the two upper internodes with a cork borer. A mycelial agar plug, from a 3-weeks-old culture on potato dextrose agar (PDA), was placed in the wound. Wounds were sealed with parafilm and wrapped with foil paper to prevent drying. Ten rooted cuttings per cultivar were inoculated with the fungus and two others with uncolonized PDA plugs, as negative controls. After inoculating, rooted cuttings were planted in a plot and irrigated by a drip system with two drippers per plant. Plants were collected after eight months and inspected for lesion development. Extent of wood necrosis was measured upward and downward from the inoculation point. Three rooted cuttings for each cultivar were selected and small pieces, of necrotic tissue from de edge of each lesion, were cut and placed on malt extract agar supplemented with 0.5 g/L of streptomycin sulphate (MEAS), in an attempt to recover the inoculated fungus and complete Koch’s postulates. N. parvum was identified by morphological and molecular approaches. Mean percentage of infected rooted cuttings ranged from 42.1% (“Monastrell” cultivar) to 93.3% (“Tinto Velasco” cultivar). Mean lengths of the extent of wood necrosis caused by N. parvum on inoculated one-year-old grapevine wood ranged from 21.2 (“Bobal” cultivar) to 87.2 mm (“Tinto Velasco” cultivar). N. parvum was reisolated from the edge of each lesion in 90.3% of the cultivars. The results of statistical analysis showed that “Bobal” and “Monastrell” cultivars were significantly more tolerant than “Tinto Velasco”.  

Conclusions:

All tested grapevine cultivars were susceptible to infection by N. parvum, evidencing that there was no qualitative resistance to this fungus. “Bobal” and “Monastrell” cultivars highlighted for their lower wood response susceptibility to N. parvum.

Significance and Impact of the Study: Interactions between Botryosphaeriaceae species and grapevine cultivars are poorly understood and there is currently little data available. This study allowed classifying different Vitis vinifera cultivars based on their degree of quantitative resistance to N. parvum. “Bobal” and “Monastrell” cultivars could be potential candidates to create tolerant varieties to N. parvum fungus. Using tolerant varieties would be the safest, easiest, the least expensive and the most effective means of controlling this disease.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Juan Luis Chacón1*, David Gramaje2, Adela Mena1, Pedro Miguel Izquierdo1, Jesús Martínez1

1Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de Castilla-La Mancha (IRIAF), Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Spain
2Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain

Contact the author

Keywords

Botryosphaeria dieback, grapevine, grapevine trunk diseases, Neofusicoccum parvum

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Grapevine sugar concentration model in the Douro Superior, Portugal

Increasingly warm and dry climate conditions are challenging the viticulture and winemaking sector. Digital technologies and crop modelling bear the promise to provide practical answers to those challenges. As viticultural activities strongly depend on harvest date, its early prediction is particularly important, since the success of winemaking practices largely depends upon this key event, which should be based on an accurate and advanced plan of the annual cycle. Herein, we demonstrate the creation of modelling tools to assess grape ripeness, through sugar concentration monitoring. The study area, the Portuguese Côa valley wine region, represents an important terroir in the “Douro Superior” subregion. Two varieties (cv. Touriga Nacional and Touriga Franca) grown in five locations across the Côa Region were considered. Sugar accumulation in grapes, with concentrations between 170 and 230 g l-1, was used from 2014 to 2020 as an indicator of technological maturity conditioned by meteorological factors. The climatic time series were retrieved from the EU Copernicus Service, while sugar data were collected by a non-profit organization, ADVID, and by Sogrape, a leading wine company. The software for calibrating and validating this model framework was the Phenology Modeling Platform (PMP), version 5.5, using Sigmoid and growing degree-day (GDD) models for predictions. The performance was assessed through two metrics: Roots Mean Square Error (RMSE) and efficiency coefficient (EFF), while validation was undertaken using leave-one-out cross-validation. Our findings demonstrate that sugar content is mainly dependent on temperature and air humidity. The models achieved a performance of 0.65

Spatial determination of areas in the Western Balkans region favorable for organic production

In problematic conditions for production of grapes and wine caused by the COVID-19 pandemic and the resulting occurrence of wine surpluses, producers are increasingly turning to the innovative viticulture and winemaking of products that are more appealing to the market and the consumers. On the other hand, consumption of the food safety or organic products, and therefore of organic grapes and wine, is increasingly common in the world, in particular in Europe. The Regional Rural Development Standing Working Group (SWG RRD), as a regional intergovernmental organization gathers actors in the viticulture and winemaking sector from states and territories of the Western Balkans (South-East Europe) in the Expert Working Group for Wine, with the aim of improving viticulture and winemaking in this region through joint activities. In accordance with the aforementioned, the SWG RRD is working on advancing organic production of grapes and wine, and on recognition of specificities of the terroir of wine-growing areas in Western Balkans. In addition, as part of the project “Facilitation of Exchange and Advice on Wine Regulations in Western Balkan Countries” helmed by the German Federal Ministry of Food and Agriculture, in addition to harmonization of relevant legislation with EU regulations, efforts are being invested towards recognition of organic wines. Within activities and project implemented by this organization, expert analyses and scientific research of the terroir of Western Balkans were carried out, and some of the results are presented in this paper.

Climate change projections to support the transition to climate-smart viticulture

The Earth’s system is undergoing major changes through a wide range of spatial and temporal scales as a response to growing anthropogenic radiative forcing, which is pushing the whole system far beyond its natural variability. Sources of greenhouse gases largely exceed their sinks, thus leading to a strengthened greenhouse effect. More energy is thereby being supplied to the system, with inevitable shifts in climatic patterns and weather regimes. Over the last decades, these modifications have been manifested in the full statistical distributions of the atmospheric variables, with dramatic changes in the frequency and intensity of extremes. Natural hazards, such as severe droughts, floods, forest fires, or heatwaves, are being triggered by extreme atmospheric events worldwide, thus threatening human activities. Viticultculture is not only exposed to changing climates but is also highly vulnerable, as grapevine phenology and physiological development are strongly controlled by atmospheric conditions. Therefore, the assessment of climate change projections for a given region is critical for climate change adaptation and risk reduction in viticulture. By adopting timely and suitable measures, the future sustainability and resiliency of the sector can be fostered. Climate-grapevine chain modelling is an essential tool for better planning and management. However, the accuracy of the resulting projections is limited by many uncertainties that must be duly taken into account when transferring knowledge to stakeholders and decision-makers. Climate-smart viticulture will comprise ensembles of locally tuned strategies, envisioning both adaptation and mitigation, assisted by emerging technologies and decision-support systems.

Combining effect of leaf removal and natural shading on grape ripening under two irrigation strategies in Manto negro (Vitis vinifera L.)

The increasingly frequent heat waves during grape ripening pose challenges for high quality wine grape production. Defoliation is a common practice that can improve the control of diseases in bunches, but also it increases the exposure to sunlight. Grapes exposed to solar radiation reach temperatures over the optimum for berry development and maturation. This makes the development of irrigation and canopy management techniques of great importance to maximize yield and grape quality. A field experiment was carried out during 2021 using Manto negro wine grapes to study the effect of applied irrigation and different light exposure levels on grape quality. Two irrigation treatments were imposed based on the frequency and amount of water doses in a four-block experimental vineyard at Bodega Ribas (Mallorca). Three light exposure treatments were randomly applied in each irrigation plot. The light treatments included exposed clusters from pea size, non-exposed clusters, and shaded clusters after softening. Leaf area index and canopy porosity was estimated every 2 weeks. Midday leaf water potential was measured weekly. Additionally, apparent electrical conductivity was measured between rows to estimate the soil water content variability. Light and temperature sensors were installed at the bunch level to quantify the differences in bunch temperature and light intensity among treatments. The effect of irrigation and cluster light exposure on berry weight, TSS, TA, malic acid, tartaric acid, K+, and pH were analysed at 5 moments along grape ripening. During different heat waves, the natural shading technique decreased the maximum bunch temperature around 10 °C respect to the exposed bunches in both irrigation strategies. The combination of defoliation and shading techniques after softening decreased TSS at harvest and affected most of the quality parameters during the last stages of ripening, showing an interesting technique to delay ripening in warm viticulture areas.

A predictive model of spatial Eca variability in the vineyard to support the monitoring of plant status

[lwp_divi_breadcrumbs home_text="IVES" use_before_icon="on" before_icon="||divi||400" module_id="publication-ariane" _builder_version="4.19.4" _module_preset="default" module_text_align="center" module_font_size="16px" text_orientation="center"...