Terroir 2020 banner
IVES 9 IVES Conference Series 9 Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum in a “terroir” of La Mancha wine región (Spain)

Evaluation of six red grapevine cultivars inoculated with Neofusicoccum parvum in a “terroir” of La Mancha wine región (Spain)

Abstract

Aim: Among Botryosphaeriaceae species associated with Botryosphaeria dieback of grapevines, Neofusicoccum parvum is one of the most virulent and fastest wood-colonizing fungi. This study aimed to evaluate the susceptibility of six red grapevine cultivars (“Bobal”, “Monastrell”, “Garnacha Tinta”, “Moravia Agria”, “Tinto Velasco” and “Moribel” to N. parvum, under field conditions.

Methods and Results: Pathogenicity studies were conducted, over two consecutive years, with one-year old grapevine rooted cuttings inoculated with a N. parvum isolate. Rooted cuttings were wounded between the two upper internodes with a cork borer. A mycelial agar plug, from a 3-weeks-old culture on potato dextrose agar (PDA), was placed in the wound. Wounds were sealed with parafilm and wrapped with foil paper to prevent drying. Ten rooted cuttings per cultivar were inoculated with the fungus and two others with uncolonized PDA plugs, as negative controls. After inoculating, rooted cuttings were planted in a plot and irrigated by a drip system with two drippers per plant. Plants were collected after eight months and inspected for lesion development. Extent of wood necrosis was measured upward and downward from the inoculation point. Three rooted cuttings for each cultivar were selected and small pieces, of necrotic tissue from de edge of each lesion, were cut and placed on malt extract agar supplemented with 0.5 g/L of streptomycin sulphate (MEAS), in an attempt to recover the inoculated fungus and complete Koch’s postulates. N. parvum was identified by morphological and molecular approaches. Mean percentage of infected rooted cuttings ranged from 42.1% (“Monastrell” cultivar) to 93.3% (“Tinto Velasco” cultivar). Mean lengths of the extent of wood necrosis caused by N. parvum on inoculated one-year-old grapevine wood ranged from 21.2 (“Bobal” cultivar) to 87.2 mm (“Tinto Velasco” cultivar). N. parvum was reisolated from the edge of each lesion in 90.3% of the cultivars. The results of statistical analysis showed that “Bobal” and “Monastrell” cultivars were significantly more tolerant than “Tinto Velasco”.  

Conclusions:

All tested grapevine cultivars were susceptible to infection by N. parvum, evidencing that there was no qualitative resistance to this fungus. “Bobal” and “Monastrell” cultivars highlighted for their lower wood response susceptibility to N. parvum.

Significance and Impact of the Study: Interactions between Botryosphaeriaceae species and grapevine cultivars are poorly understood and there is currently little data available. This study allowed classifying different Vitis vinifera cultivars based on their degree of quantitative resistance to N. parvum. “Bobal” and “Monastrell” cultivars could be potential candidates to create tolerant varieties to N. parvum fungus. Using tolerant varieties would be the safest, easiest, the least expensive and the most effective means of controlling this disease.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Juan Luis Chacón1*, David Gramaje2, Adela Mena1, Pedro Miguel Izquierdo1, Jesús Martínez1

1Instituto Regional de Investigación y Desarrollo Agroalimentario y Forestal de Castilla-La Mancha (IRIAF), Ctra. Toledo-Albacete s/n, 13700 Tomelloso, Spain
2Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas – Universidad de la Rioja – Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071 Logroño, Spain

Contact the author

Keywords

Botryosphaeria dieback, grapevine, grapevine trunk diseases, Neofusicoccum parvum

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.

The interplay between grape ripening and weather anomalies – A modeling exercise

Current climate change is increasing inter- and intra-annual variability in atmospheric conditions leading to grapevine phenological shifts as well altered grape ripening and composition at ripeness. This study aims to (i) detect weather anomalies within a long-term time series, (ii) model grape ripening revealing altered traits in time to target specific ripeness thresholds for four Vitis vinifera cultivars, and (iii) establish empirical relationships between ripening and weather anomalies with forecasting purposes. The Day of the Year (DOY) to reach specific grape ripeness targets was determined from time series of sugar concentrations, total acidity and pH collected from a private company in the period 2009-2021 in North-Eastern Italy. Non-linear models for the DOY to reach the specified ripeness thresholds were assessed for model efficiency (EF) and error of prediction (RMSE) in four grapevine cultivars (Merlot, Cabernet Sauvignon, Glera and Garganega). For each vintage and cultivar, advances or delays in DOY to target specified ripeness thresholds were assessed with respect to the average ripening dynamics. Long-term meteorological series monitored at ground weather station by means of hourly air temperature and rainfall data were analyzed. Climate statistics were obtained and for each time period (month, bimester, quarter and year) weather anomalies were identified. A linear regression analysis was performed to assess a possible correlation that may exist between ripening and weather anomalies. For each cultivar, ripeness advances or delays expressed in number of days to target the specific ripening threshold were assessed in relation to registered weather anomalies and the specific reference time period in the vintage. Precipitation of the warmest month and spring quarter are key to understanding the effect of climate change on sugar ripeness. Minimum temperatures of May-June bimester and maximum temperatures of spring quarter best correlate with altered total acidity evolution and pH increment during the ripening process, respectively.

Green berries on Gewürztraminer (Vitis vinifera L.) in South Tyrol (Italy)

The grape variety Gewürztraminer is known to be affected by two physiological disorders namely berry shrivel and bunch stem necrosis. During the season 2014 we noticed a new symptomatology type of ripening disorder on the variety. The new symptom showed not all berries fallowing the normal maturation stages, but single berries remaining at a soft but green stage till harvest. The broad distribution of these so called “green berries” symptoms in different production sites of our region, caused huge damage due to the difficulty of eliminating single berries per bunch before harvesting. Therefore, the Research Centre Laimburg began to investigate the reasons and origins of this new symptom. This work shows the results of first attempts to find causes for the symptom as well as the resulting approach to mitigate symptoms. Applications of magnesium leaf fertilizer showed first promising results against this putative disorder. To study the causal effect of the green berries 30 symptomatic vineyards in 2014 have been selected for a monitoring during the season 2016. To evaluate the foliar nutrient treatment two vineyards have been selected for application of magnesium sulfate and magnesium chloride. Leaf and berry nutrient analysis, as well as the main quality parameters during ripening have been performed. As soon as “green berries” symptoms appeared, incidence and severity have been evaluated. Most of the symptomatic vineyards of the 2016 monitoring showed light to clear magnesium deficit symptoms on their foliage. Only during the seasons 2020 and 2021 “green berries” symptoms could be found in the leaf fertilizer treatment vineyards. Both seasons showed a significant effect of the magnesium treatments to reduce the incidence and severity of the symptom. It seems that the appearance of the “green berries” symptom on Gewürztraminer is correlated to a disturbed uptake of magnesium of the vines.

Differential responses of red and white grape cultivars trained to a single trellis system – the VSP

Commercial grape production relies on training grapevine cultivars onto a variety of trellis systems. Training allows for well-lit leaves and clusters, maximizing fruit quality in addition to facilitating cultivation, harvesting, and diseases control. Although grapevines can be trained onto an infinite variety of trellis systems, most red and white cultivars are trained to the standard VSP (Vertical Shoot Positioning) system. However, red and white cultivars respond differently to VSP in fruit composition and growth characteristics, which are yet to be fully understood. Therefore, the objective of this study was to examine the influence of the VSP trellis system on fruit composition of three red, Cabernet Sauvignon, Merlot and Syrah, and three white, Chardonnay, Riesling, and Gewurztraminer cultivars grown under uniform growing conditions in the same vineyard. All cultivars were monitored for maturity and harvested at their physiologically maximum possible sugar concentration to compare various fruit quality attributes such as Brix, pH, TA, malic and tartaric acids, glucose and fructose, potassium, YAN, and phenolic compounds including total anthocyanins, anthocyanin profile, and tannins. A distinct pattern in fruit composition was observed in each cultivar. In regards to growth characteristics, Syrah grew vigorously with the highest cluster weight. Although all cultivars developed pyriform seeds, the seed size and weight varied among all cultivars. Also varied were mesocarp cell viability, brush morphology, and cane structure. This knowledge of the canopy architectural characteristics assessed by the widely employed fruit compositional attributes and growth characteristics will aid the growers in better management of the vines in varied situations.

A multidisciplinary approach to evaluate the effects of the training system on the performance of “Aglianico del Vulture” vineyards

Vineyards are complex agro-ecosystems with high spatial and temporal variability. An efficient training system may counteract the adverse effects of this variability. Moreover, considering the climate change issues, choosing an efficient training system that enhances water use and protects the vines from radiative thermal stress has become a priority for the farmers. A multidisciplinary approach that assesses the soil-crop-yield-wine relationships of vineyards in a distributed and holistic way could bring added knowledge on the behavior of the different training systems. This ongoing research aimed to implement a multidisciplinary approach to study the behavior of “Aglianico del Vulture” grapevines trained with two different systems: a spurred cordon (SC) and an “Alberello in parete” (AL), grown in a high-quality wine production area of Basilicata region (Italy). The approach merged several methods and scales of soil, ecophysiology, must/wine quality, and spectral data collection to assess the influence of the training system. Homogeneous zones (HZs) in both training systems were defined through a procedure based on geomorphological classification, unmanned aerial vehicles (UAV) images analysis, and a traditional soil survey supported by geophysical scanning. During the 2021 season, TDR probes monitored soil water content, while grapevine health status was assessed using eco-physiological measurements (LWP, chlorophyll content, PSII photosynthetic efficiency, LAI, and point-based field spectroscopy). These grapevine in-vivo measurements validated the spectral vegetation indexes (NDVI, RENDVI, CVI, and TVI) derived from the UAV multispectral imagery, which monitored the grapevine status in a distributed and non-invasive way. Grape yield, quality of berries, must and wine were measured to assess the effects of the training systems. The first experimental year results showed the variability of the vineyards and revealed relationships among soil parameters, crop characteristics, and vegetation indices of the SC and AL training systems. This multidisciplinary study could bring new insights into the vineyard training system’s effects on grape yield and wine quality.