Terroir 2020 banner
IVES 9 IVES Conference Series 9 The vascular connections in grafted plants under examination

The vascular connections in grafted plants under examination

Abstract

Aims: Decreasing longevity of vineyards due to the increase in the infection of different grapevine trunk diseases is a growing concern, and could be related to the quality of grafting. The main aim of this study was to evaluate the use of xylem hydraulic conductivity measurements as a potential indicator for the quality of vascular connections in the graft junction of grapevine plants. For that purpose, two specific trials were carried out: (1) the quality of different batches of plants whose subjective quality was previously known from the nursery’s experience was evaluated, and (2) the vascular connections between the rootstock and the scion were evaluated on a different set of plants grafted using different grafting techniques.

Methods and Results: (1) The XYL´EM conductivity meter was used to measure the specific hydraulic conductivity (Ks) of plants from six different batches whose quality was subjectively defined from the nurseryman experience. Hydraulic conductivity was measured in one-year-old dormant plants, which were kept in a cold chamber since leaving the nursery, first on the whole plant, then on the rootstock and finally at the graft junction. Results showed that two of the six batches evaluated had significantly lower values than the others. (2) After reflecting on the previous experience, we decided to perform the measurements in growing plants, for which we used different batches of plants grafted using different techniques but keeping the same scion (Airén) and the same rootstock (110 R) in all of them. The grafting techniques used were ‘omega’, ‘full cleft’, ‘manual whip and tongue’, ‘mechanical whip and tongue’, and ‘V’. This time, Ksmeasurements were only carried out on the whole plant by determining the water flow from the scion to the rootstock. Results revealed a tendency that the maWT, meWT and V grafts had higher Ks values than FC or OM grafts, which corresponds to higher growth rates according to results previously collected on a field trial performed on the same type of grafted plants.

Conclusions:

Hydraulic conductivity measurements may be a useful trait for the evaluation of vascular connections between the rootstock and the scion in grapevine.

Significance and Impact of the Study: To the best of our knowledge, there are no similar studies in grapevine combining hydraulics measurements with grafting techniques. Results suggest that the hydraulic functioning at the graft junction could be an interesting tool to measure the quality/quantity of vascular connections on grafted plants, and be used to characterize batches in experimentation or, even, to evaluate batches from the nurseries as a quality control.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Diana Marín1*, José M. Torres-Ruiz2, F. Javier Abad1, 3, Luis G. Santesteban1

Dept. of Agronomy, Biotechnology and Food Science, Univ. Pública de Navarra, Campus Arrosadia, 31006 Pamplona, Spain
Université Clermont-Auvergne, INRAE, PIAF, 63000 Clermont-Ferrand, France
3 INTIA, Edificio de Peritos Avda. Serapio Huici nº 22, 31610, Villava, Spain

Contact the author

Keywords

Grapevine, Vitis vinifera L., omega graft, whip and tongue, alternative graft

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Grapevine yield estimation in a context of climate change: the GraY model

Grapevine yield is a key indicator to assess the impacts of climate change and the relevance of adaptation strategies in a vineyard landscape. At this scale, a yield model should use a number of parameters and input data in relation to the information available and be able to reproduce vineyard management decisions (e.g. soil and canopy management, irrigation). In this study, we used data from six experimental sites in Southern France (cv. Syrah) to calibrate a model of grapevine yield limited by water constraint (GraY). Each yield component (bud fertility, number of berries per bunch, berry weight) was calculated as a function of the soil water availability simulated by the WaLIS water balance model at critical phenological phases. The model was then evaluated in 10 grapegrowers’ plots, covering a diversity of biophysical and technical contexts (soil type, canopy size, irrigation, cover crop). We identified three critical periods for yield formation: after flowering on the previous year for the number of bunches and berries, around pre-veraison and post-veraison of the same year for mean berry weight. Yields were simulated with a model efficiency (EF) of 0.62 (NRMSE = 0.28). Bud fertility and number of berries per bunch were more accurately simulated (EF = 0.90 and 0.77, NRMSE = 0.06 and 0.10, respectively) than berry weight (EF = -0.31, NRMSE = 0.17). Model efficiency on the on-farm plots reached 0.71 (NRMSE = 0.37) simulating yields from 1 to 8 kg/plant. The GraY model is an original model estimating grapevine yield evolution on the basis of water availability under future climatic conditions.  It allows to evaluate the effects of various adaptation levers such as planting density, cover crop management, fruit/leaf ratio, shading and irrigation, in various production contexts.

Bioclimatic shifts and land use options for Viticulture in Portugal

Land use, plays a relevant role in the climatic system. It endows means for agriculture practices thus contributing to the food supply. Since climate and land are closely intertwined through multiple interface processes, climate change may lead to significant impacts in land use. In this study, 1-km observational gridded datasets are used to assess changes in the Köppen–Geiger and Worldwide Bioclimatic (WBCS)

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Grape berry size is a key factor in determining New Zealand Pinot noir wine composition

Making high quality but affordable Pinot noir (PN) wine is challenging in most terroirs and New Zealand’s (NZ) situation is no exception. To increase the probability of making highly typical PN wines producers choose to grow grapes in cool climates on lower fertility soils while adopting labour intensive practices. Stringent yield targets and higher input costs necessarily mean that PN wine cost is high, and profitability lower, in line-priced varietal wine ranges. To understand the reasons why higher yielding vines are perceived to produce wines of lower quality we have undertaken an extensive study of PN in NZ. Since 2018, we established a network of twelve trial sites in three NZ regions to find individual vines that produced acceptable commercial yields (above 2.5kg per vine) and wines of composition comparable to “Icon” labels. Approximately 20% of 660 grape lots (N = 135) were selected from within a narrow juice Total Soluble Solids (TSS) range and made into single vine wines under controlled conditions. Principal Component Analysis of the vine, berry, juice and wine parameters from three vintages found grape berry mass to be most effective clustering variable. As berry mass category decreased there was a systematic increase in the probability of higher berry red colour and total phenolics with a parallel increase in wine phenolics, changed aroma fraction and decreased juice amino acids. The influence of berry size on wine composition would appear stronger than the individual effects of vintage, region, vineyard or vine yield. Our observations support the hypothesis that it is possible to produce PN wines that fall within an “Icon” benchmark composition range at yields above 2.5kg per vine provided that the Leaf Area:Fruit Weight ratio is above 12cm2 per g, mean berry mass is below 1.2g and juice TSS is above 22°Brix.

Effect of vigour and number of clusters on eonological parameters and metabolic profile of Cabernet Sauvignon red wines

Vegetative growth and yield are reported to affect grape and wine quality. They can be controlled through different techniques linked to vine management. The objective of this research was to determine the effect of vine vigour and number of clusters per vine on physicochemical composition and phenolic profile of red wines. The experiment was carried out during two vegetative cycles, with cv. Cabernet Sauvignon grafted onto Paulsen 1103. Three vine vigour were defined, according to shoot weight at previous harvests, being low, medium and high. Five treatments of number of clusters were used for each vigour, with 15, 22, 29, 36, and 45 clusters per vine. Grapes from all treatments were harvested in the same day from Brix and total acidity criteria. Thirty days after bottling, classical analyzes and phenolic compounds were performed. As results, different responses were obtained from each vintage. In 2020, a dry season from veraison to harvest, grapes and wines obtained from low vigour treatment and 45 clusters per vine was the highest in sugar and alcohol content respectively, while grapes and wines from high vigour and 15 clusters presented the lowest sugar and alcohol content. Total anthocyanins were higher in treatment with low vigour and 15 clusters, while the lowest amounts were found in low vigour with 45 clusters, as well as medium and high vigour with 36 clusters per vine. Total tannins were higher in high vigour with 22 clusters and medium vigour with 29 clusters, while were lower in low vigour with 36 clusters. In 2021, a wet season at harvest, responses were different, and great variations were observed between treatments. As conclusions, yield and vine vigour had strong influence on grape and wine quality, promoting different enological potentials on which can be indicated/used for aging strategies of red and even rosé wines.