Terroir 2020 banner
IVES 9 IVES Conference Series 9 Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Abstract

Aim: This study aimed to evaluate whether irrigation of Touriga Nacional in Douro Demarcated Region (DDR) can partly mitigate the negative impacts of ongoing climate change on grapevine yield and quality and its impact on plant metabolism.

Methods and results: Water status was determined by performing pre-dawn leaf water potential (ΨPd) using a pressure chamber throughout the growing season. Although from the end of July till the end of August of 2018 both R30 and R70 significantly prevented a decay of ΨPd under extreme drought conditions, R30 promoted only a relatively small increase of yield at harvest, but this increase was not observed at R70. In 2019, drought conditions were not so harsh than in 2018 and differences in cluster weights were not observed among irrigation treatments at harvest. A UPLC-MS-based targeted metabolomic analysis from the vintage 2018 identified 44 compounds in grapes from non-irrigated (R0), irrigated at 30% of evapotranspiration (ETc; R30) and 70% ETc (R70), corresponding to eight classes: amino acids; phenolic acids; stilbenoid DP1; stilbenoid DP2; flavonols; flavan-3-ols; di-OH anthocyanins and tri-OH anthocyanins. PCA analysis showed that irrigation influenced the composition of the different classes of grape berry compounds e.g. amino acids, phenolic acids, stilbenoids, flavonols, flavan-3-ols, and anthocyanins.

Conclusions:

In the two consecutive seasons of 2018 and 2019 in DDR irrigation at R30 and R70 failed to bring Touriga Nacional vines to hydric comfort at veraison, when drought stress was more pronounced, and did not substantially affect yield and berry quality traits at harvest. However, UPLC-MS-base metabolomics analyses highlighted that berry metabolism was tuned under different irrigation regimes, but more water did not traduce in higher contents of key metabolites like anthocyanins

Significance and Impact of the Study: Douro Demarcated Region (DDR) has a Mediterranean climate with low rainfall values during summer, high temperatures and high levels of radiation. The introduction of irrigation in this region is still a matter of debate due to the limited number of available studies.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Inês Cabral1*, António  Teixeira2, Arnaud  Lanoue3, Marianne  Unlubayir3, Thibaut  Munsch3, Joana  Valente4, Fernando  Alves4, Pedro  Costa4, Frank  Rogerson4, Susana  Carvalho1, Hernâni  Gerós2,5,6, Anabela  Carneiro1, Jorge  Queiroz1

1GreenUPorto – Research Centre for Sustainable Agrifood Production & DGAOT, Faculty of Sciences, University of Porto, Vairão, Portugal
2Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Gualtar, Portugal
3Université de Tours, EA2106 Biomolécules et Biotechnologie Végétales, Tours, France
4Symington Family Estates, Vila Nova de Gaia, Portugal
5Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
6Centre of Biological Engineering (CEB), Department of Biological Engineering, University of Minho, Gualtar, Portugal

Contact the author

Keywords

Deficit irrigation, metabolomics, leaf water potential, grape quality

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

Zonage vitivinicole: recherches et considérations initiales sur une proposition de “nouvelle” méthodologie d'”évaluation de la qualité” du produit tel qu’élément base pour le zonage aussi

Si on part de l’introduction que l’activité vitivinicole maintenant plus que jamais doit être une activité d’entreprenariat introduite de mieux en mieux sur le territoire et donc effectuée pour rendre maximal le Profit

Climate change and viticulture in Nordic Countries and the Helsinki area

The first vineyards in Northern Europe were in Denmark in the 15th century, in the southern parts of Sweden and Finland in the 18th century at 55–60 degrees latitude. The grapes grown there have not been made into wine, but the grapes have been eaten at festive tables. The resurgence of viticulture has started with global warming, and currently the total area of viticulture in the Nordic countries, including Norway, is estimated to be 400–500 hectares, most of which is in Denmark. Southern Finland, like all southern parts of Northern Europe, belongs to the cool-cold winegrowing area.

Isolation of indigenous yeast strains from the Purcari and Trifești wine centers in the Republic of Moldova and evaluation of their impact on the quality of dry red wines

In the conducted research, 30 yeast strains from red grape varieties were isolated from the Purcari wine center, and 28 yeast strains from red grape varieties were isolated from the Trifești wine center in the Republic of Moldova.

Sustainable geographical indications? Inclusion of sustainability criteria in the Denomination of Origin Campos de Cima da Serra, Brazil

The objective of this study is to assess the potential for integrating sustainability guidelines into Geographical Indications of wine, especially in the case of the Denomination of Origin Campos de Cima da Serra (CCS), Brazil.