Terroir 2020 banner
IVES 9 IVES Conference Series 9 Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Abstract

Aim: This study aimed to evaluate whether irrigation of Touriga Nacional in Douro Demarcated Region (DDR) can partly mitigate the negative impacts of ongoing climate change on grapevine yield and quality and its impact on plant metabolism.

Methods and results: Water status was determined by performing pre-dawn leaf water potential (ΨPd) using a pressure chamber throughout the growing season. Although from the end of July till the end of August of 2018 both R30 and R70 significantly prevented a decay of ΨPd under extreme drought conditions, R30 promoted only a relatively small increase of yield at harvest, but this increase was not observed at R70. In 2019, drought conditions were not so harsh than in 2018 and differences in cluster weights were not observed among irrigation treatments at harvest. A UPLC-MS-based targeted metabolomic analysis from the vintage 2018 identified 44 compounds in grapes from non-irrigated (R0), irrigated at 30% of evapotranspiration (ETc; R30) and 70% ETc (R70), corresponding to eight classes: amino acids; phenolic acids; stilbenoid DP1; stilbenoid DP2; flavonols; flavan-3-ols; di-OH anthocyanins and tri-OH anthocyanins. PCA analysis showed that irrigation influenced the composition of the different classes of grape berry compounds e.g. amino acids, phenolic acids, stilbenoids, flavonols, flavan-3-ols, and anthocyanins.

Conclusions:

In the two consecutive seasons of 2018 and 2019 in DDR irrigation at R30 and R70 failed to bring Touriga Nacional vines to hydric comfort at veraison, when drought stress was more pronounced, and did not substantially affect yield and berry quality traits at harvest. However, UPLC-MS-base metabolomics analyses highlighted that berry metabolism was tuned under different irrigation regimes, but more water did not traduce in higher contents of key metabolites like anthocyanins

Significance and Impact of the Study: Douro Demarcated Region (DDR) has a Mediterranean climate with low rainfall values during summer, high temperatures and high levels of radiation. The introduction of irrigation in this region is still a matter of debate due to the limited number of available studies.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Inês Cabral1*, António  Teixeira2, Arnaud  Lanoue3, Marianne  Unlubayir3, Thibaut  Munsch3, Joana  Valente4, Fernando  Alves4, Pedro  Costa4, Frank  Rogerson4, Susana  Carvalho1, Hernâni  Gerós2,5,6, Anabela  Carneiro1, Jorge  Queiroz1

1GreenUPorto – Research Centre for Sustainable Agrifood Production & DGAOT, Faculty of Sciences, University of Porto, Vairão, Portugal
2Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Gualtar, Portugal
3Université de Tours, EA2106 Biomolécules et Biotechnologie Végétales, Tours, France
4Symington Family Estates, Vila Nova de Gaia, Portugal
5Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
6Centre of Biological Engineering (CEB), Department of Biological Engineering, University of Minho, Gualtar, Portugal

Contact the author

Keywords

Deficit irrigation, metabolomics, leaf water potential, grape quality

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Effect Of Grape Polysaccharides On The Volatile Composition Of Red Wines

Yeast mannoproteins and derivates are polysaccharides produced from the cell walls of different yeast strains widely used in the winemaking and finning of wines to improve their overall stability and sensory properties.

Wine without added SO2: Oxygen impact and color evolution during red wine aging

SO2 play a major role in wine stability and evolution during its aging and storage. Winemaking without SO2 is a big challenge for the winemakers since the lack of SO2 affects directly the wine chemical evolution such as the aromas compounds as well as the phenolic compounds. During the red wine aging, phenolic compounds such as anthocyanin, responsible of the red wine colour, and tannins, responsible of the mouthfeel organoleptic properties of wine, evolved quickly from the winemaking process to aging [1]. A lot of new interaction and molecules occurred lead by oxygen[2] thus the lack of SO2 will induce wine properties changes. Nowadays, the phenolic composition of the wine without added SO2 have not been clearly reported.

Genetic causes of SO2 consumption in Saccharomyces cerevisiae

SO2 is used during winemaking for its anti-oxidative and anti-microbial properties. A high SO2 concentration in the wine has negative impacts by hiding wine aromas and delaying malolactic fermentation.

The role of tomato juice serum in malolactic fermentation in wine

Introduction: Malolactic fermentation (MLF) is a common process in winemaking to reduce wine acidity, maintain microbial stability and modify wine aroma. However, successful MLF is often hampered by their sluggish or stuck activity of malolactic bacteria (MLB) which may be caused by nutrient deficiency, especially when MLB are inoculated after alcoholic fermentation (Alexandre et al., 2004; Lerm et al., 2010). Identification and characterization of essential nutrients and growth factors for MLB allows for production of highly efficient nutrient supplements for MLF.

Phenological stage dependency of Cabernet Sauvignon and Grenache response to water and nutrient limitation 

As the frequency and intensity of drought events increase, understanding the mechanisms of plant resilience to water deficit is crucial. To maintain an appropriate plant yield, a common practice is the application of high amounts of fertilizers with negative environmental impacts. The single and combined effect of water deficit and nutrient availability, namely nitrogen (N) and potassium (K), in Vitis Vinifera L. cv. Cabernet Sauvignon and Grenache was evaluated. Two-year-old grapevine plants grafted on SO4 rootstock were transferred in pots under semi-environmental conditions. During the growing season, plants were either maintained well-watered (100% ETc) or subjected to a controlled water deficit irrigation (33% ETc).