Terroir 2020 banner
IVES 9 IVES Conference Series 9 Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Influence of deficit irrigation on grapevine cv. “Touriga Nacional” in Douro region: A metabolomic approach

Abstract

Aim: This study aimed to evaluate whether irrigation of Touriga Nacional in Douro Demarcated Region (DDR) can partly mitigate the negative impacts of ongoing climate change on grapevine yield and quality and its impact on plant metabolism.

Methods and results: Water status was determined by performing pre-dawn leaf water potential (ΨPd) using a pressure chamber throughout the growing season. Although from the end of July till the end of August of 2018 both R30 and R70 significantly prevented a decay of ΨPd under extreme drought conditions, R30 promoted only a relatively small increase of yield at harvest, but this increase was not observed at R70. In 2019, drought conditions were not so harsh than in 2018 and differences in cluster weights were not observed among irrigation treatments at harvest. A UPLC-MS-based targeted metabolomic analysis from the vintage 2018 identified 44 compounds in grapes from non-irrigated (R0), irrigated at 30% of evapotranspiration (ETc; R30) and 70% ETc (R70), corresponding to eight classes: amino acids; phenolic acids; stilbenoid DP1; stilbenoid DP2; flavonols; flavan-3-ols; di-OH anthocyanins and tri-OH anthocyanins. PCA analysis showed that irrigation influenced the composition of the different classes of grape berry compounds e.g. amino acids, phenolic acids, stilbenoids, flavonols, flavan-3-ols, and anthocyanins.

Conclusions:

In the two consecutive seasons of 2018 and 2019 in DDR irrigation at R30 and R70 failed to bring Touriga Nacional vines to hydric comfort at veraison, when drought stress was more pronounced, and did not substantially affect yield and berry quality traits at harvest. However, UPLC-MS-base metabolomics analyses highlighted that berry metabolism was tuned under different irrigation regimes, but more water did not traduce in higher contents of key metabolites like anthocyanins

Significance and Impact of the Study: Douro Demarcated Region (DDR) has a Mediterranean climate with low rainfall values during summer, high temperatures and high levels of radiation. The introduction of irrigation in this region is still a matter of debate due to the limited number of available studies.

DOI:

Publication date: March 25, 2021

Issue: Terroir 2020

Type : Video

Authors

Inês Cabral1*, António  Teixeira2, Arnaud  Lanoue3, Marianne  Unlubayir3, Thibaut  Munsch3, Joana  Valente4, Fernando  Alves4, Pedro  Costa4, Frank  Rogerson4, Susana  Carvalho1, Hernâni  Gerós2,5,6, Anabela  Carneiro1, Jorge  Queiroz1

1GreenUPorto – Research Centre for Sustainable Agrifood Production & DGAOT, Faculty of Sciences, University of Porto, Vairão, Portugal
2Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Gualtar, Portugal
3Université de Tours, EA2106 Biomolécules et Biotechnologie Végétales, Tours, France
4Symington Family Estates, Vila Nova de Gaia, Portugal
5Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, Vila Real, Portugal
6Centre of Biological Engineering (CEB), Department of Biological Engineering, University of Minho, Gualtar, Portugal

Contact the author

Keywords

Deficit irrigation, metabolomics, leaf water potential, grape quality

Tags

IVES Conference Series | Terroir 2020

Citation

Related articles…

Better understand the soil wet bulb formation with subsurface or aerial drip irrigation in viticulture

The gradual change in rainfall patterns experienced in the south of France vineyards, especially around the Mediterranean sea, means that the vines are increasingly subject to summer drought. The winegrowers developped the use of irrigation techniques to ensure the maintenance of competitive yields in the production of wines under Protected Geographical Indication label. In practice, drip irrigation pipes can be installed above the ground or buried into the soil as well as at different distances from the vine row. The objective of this study was to examine the profiles of the wet bulbs of the soil obtained from two drip irrigation systems : aerial drip located under the vine row and subsurface drip placed in the middle of the inter-row. This experiment took place over two consecutive seasons (2020-2021) on a 3.4 ha Viognier plot in the Mediterranean region (PGI Oc, France) on sandy clay soil. The annual rainfalls were less than 400 mm. Soil water content probes were installed at different depths (20 – 40 – 60 – 80 cm) and at different lateralities from the vine row (30 – 60 – 90 – 120 cm) to control the formation of the soil wet bulb during irrigation. The mapping and the analysis of the data allowed a better understanding and differentiation of the water percolation when irrigating with subsurface or aerial drip. For the same amount of water and without differences of vine water status, it is shown that in a subsurface drip irrigation situation, the size of the wet bulb formed is larger than in aerial drip irrigation system.

Variety and climatic effects on quality scores in the Western US winegrowing regions

Wine quality is strongly linked to climate. Quality scores are often driven by climate variation across different winegrowing regions and years, but also influenced by other aspects of terroir, including variety. While recent work has looked at the relationship between quality scores and climate across many European regions, less work has examined New World winegrowing regions. Here we used scores from three major rating systems (Wine Advocate, Wine Enthusiast and Wine Spectator) combined with daily climate and phenology data to understand what drives variation across wine quality scores in major regions of the Western US, including regions in California, Oregon and Washington. We examined effects of variety, region, and in what phenological period climate was most predictive of quality. As in other studies, we found climate, based mainly on growing degree day (GDD) models, was generally associated with quality—with higher GDD associated with higher scores—but variety and region also had strong effects. Effects of region were generally stronger than variety. Certain varieties received the highest scores in only some areas, while other varieties (e.g., Merlot) generally scored lower across regions. Across phenological stages, GDD during budbreak was often most strongly associated with quality. Our results support other studies that warmer periods generally drive high quality wines, but highlight how much region and variety drive variation in scores outside of climate.

Analysis of Cabernet Sauvignon and Aglianico winegrape (V. vinifera L.) responses to different pedo-climatic environments in southern Italy

Water deficit is one of the most important effects of climate change able to affect agricultural sectors. In general, it determines a reduction in biomass production, and for some plants, as in the case of grapevine, it can endorse fruit quality. The monitoring and management of plant water stress in the vineyard

20-Year-Old data set: scion x rootstock x climate, relationships. Effects on phenology and sugar dynamics

Global warming is one of the biggest environmental, social, and economic threats. In the Douro Valley, change to the climate are expected in the coming years, namely an increase in average temperature and a decrease in annual precipitation. Since vine cultivation is extremely vulnerable and influenced by the climate, these changes are likely to have negative effects on the production and quality of wine.
Adaptation is a major challenge facing the viticulture sector where the choice of plant material plays an important role, particularly the rootstock as it is a driver for adaptation with a wide range of effects, the most important being phylloxera, nematode and salt, tolerance to drought and a complex set of interactions in the grafted plant.
In an experimental vineyard, established in the Douro Region in 1997, with four randomized blocs, with five varieties, Touriga Nacional, Tinta Barroca, Touriga Franca and Tinta Roriz, grafted in four rootstocks, Rupestris du Lot, R110, 196-17C, R99 and 1103P, data was collected consecutively over 20 years (2001-2020). Phenological observations were made two to three times a week, following established criteria, to determine the average dates of budbreak, flowering and veraison. During maturation, weekly berry samples were taken to study the dynamics of sugar accumulation, amongst other parameters. Climate data was collected from a weather station located near the vineyard parcel, with data classified through several climatic indices.
The results achieved show a very low coefficient of variations in the average date of the phenophases and an important contribution from the rootstock in the dynamic of the phenology, allowing a delay in the cycle of up to10-12 days for the different combinations. The Principal Component Analysis performed, evaluating trends in the physical-chemical parameters, highlighted the effect of the climate and rootstock on fruit quality by grape varieties.

Simulating climate change impact on viticultural systems in historical and emergent vineyards

Global climate change affects regional climates and hold implications for wine growing regions worldwide. Although winegrowers are constantly adapting to internal and external factors, it seems relevant to develop tools, which will allow them to better define actual and future agro-climatic potentials. Within this context, we develop a modelling approach, able to simulate the impact of environmental conditions and constraints on vine behaviour and to highlight potential adaptation strategies according to different climate change scenarios. Our modeling approach, named SEVE (Simulating Environmental impacts on Viticultural Ecosystems), provides a generic modeling framework for simulating grapevine growth and berry ripening under different conditions and constraints (slope, aspect, soil type, climate variability…) as well as production strategies and adaptation rules according to climate change scenarios. Each activity is represented by an autonomous agent able to react and adapt its reaction to the variability of environmental constraints. Using this model, we have recently analyzed the evolution of vineyards’ exposure to climatic risks (frost, pathogen risk, heat wave) and the adaptation strategies potentially implemented by the winegrowers. This approach, implemented for two climate change scenarios, has been initiated in France on traditional (Loire Valley) and emerging (Brittany) vineyards. The objective is to identify the time horizons of adaptations and new opportunities in these two regions. Carried out in collaboration with wine growers, this approach aims to better understand the variability of climate change impacts at local scale in the medium and long term.