Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 X-ray tomography: a promising tool to assess the selection of good quality grafted vines

X-ray tomography: a promising tool to assess the selection of good quality grafted vines

Abstract

The production of grated vines is a complex process from grafting to final sorting in nurseries. To reach the market, grafted grapevines must meet three criteria by law in France: resistance to a manual graft union test (named thumb test), a minimum number of three roots and a woody shoot of at least 2 cm long. The thumb test is in essence unmeasurable in view of its manual and subjective execution and does not allow to evaluate the internal quality of the junctions. The development of tools and medical imaging methods may help to assess the internal quality of the graft union.

Commonly used in the medical field to identify some pathologies, X-ray tomography is also used in other fields including plant biology because of its ability to image structures in depth. Previous work on vines has shown its interest to distinguish the pith, the phloem, the xylem vessels and the necrotic tissues. We decided to investigate its ability to identify possible internal criteria relevant to the selection of good quality grafted vines prior to marketing. 

We therefore developed a specific methodology in terms of scanning parameters, 3D reconstruction and images analysis able to be used onto many plants. It was then applied onto 110 vines, Omega-grafted, just before being sorted. Different internal anatomical and functional criteria were measured in the rootstock, the scion and the graft area. Two criteria (“Quantity of xylem produced after grafting” and “Air and necrosis volume in the grafting area”) appeared interesting because they present statically different values on the batches that pass or not the sorting process. 

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Anne-Sophie Spilmont*, Camille Carrere, Yosra Hmedi and Guillaume Mathieu

Institut Français de la Vigne et du vin (IFV), Montpellier, France.

Contact the author

Keywords

grafting, 3D imaging, X-ray Tomography, nursery, graft quality

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

Evaluating the greenness of wine analytical chemistry: A new metric approach

Wine is a complex matrix whose composition depends on climatic, agricultural, and winemaking factors, making quality control and authenticity assessment critical in the global market.
Read More

Radiation-associated effects on regulated deficit irrigation management in grapevine cv. Cabernet Sauvignon

The main challenge of regulated deficit irrigation (RDI) research is to isolate the factors that come with RDI, the direct effect of plant water status from the indirect ones like increased radiation and temperature changes on the cluster zone. This study aims to isolate the effects of vine water status from the effects of increased radiation on the phenolic composition of grapes subjected to RDI.
A three-year study on an RDI experiment where radiation was controlled was implemented in a commercial vineyard of Cabernet Sauvignon in Chile. Four RDI treatments based on partial evapotranspiration (ET) irrigation were established. Irrigation treatments were 100% ET, 70% ET, 50-100% ET (50% ET before veraison and 100% ET afterward), and 35-100% ET (35% ET before veraison and 100% ET afterward).

Read More

Some applications come from a method to concentrate proteins

All techniques usually used to assay proteins was not reliable in vegetable extract due to interferences with the components included in extracts like polyphenols, tanins, pectines, aromatics compounds. Absorbance at 280nm, Kjeldhal assay, Biuret and Lowry methods, Acid Bicinchonique technique and Bradford assay give the results depending on the composition of extract, on the presence or not of detergent and on the raw material (Marchal, 1995). Another difficulty in these extracts for the quantification of proteins comes from the large amount of water included in vegetable and the low concentration of proteins. Thus in red wines, proteins are usually not taken into account due to their low concentration (typically below 10 mgL-1) and to the presence of anthocyanis and polyphenols.

Read More

Delayed irrigation nearly doubles yield loss compared to anthocyanin gain in Southern Oregon Pinot noir

Irrigation initiation timing is a critical annual management decision that has cascading effects on grapevine productivity and wine quality.

Read More

DNA-free genome editing confers disease resistance in grapevine varieties

The grapevine is facing significant challenges due to climate change, as rising temperatures impact its physiological traits and disrupt plant phenology.

Read More