Enoforum 2021
IVES 9 IVES Conference Series 9 Enoforum Web 9 Enoforum Web Conference 2021 9 Characterized one of the largest collections of grapevine rootstocks (non-vinifera)

Characterized one of the largest collections of grapevine rootstocks (non-vinifera)

Abstract

Microsatellite markers are a valuable tool to facilitate the management of germplasm collections and assess genetic diversity. This study reports the genetic characterization of a large collection of 379 rootstocks and other non-viniferaaccessions maintained at the University of Milan, Italy. Accessions were genotyped with 22 highly polymorphic microsatellite markers, including the nine “international” loci used for grapevine identification, three VMC, three VrZAG, and seven VChr loci; 17 loci were retained to identify cultivars, investigate genetic diversity, analyze pedigrees, infer population structure, and design a core collection. This study identified 232 unique genotypes; the allelic profiles of 70 rootstocks were confirmed according to the literature and databases, while the profiles of 43 rootstocks were proposed for the first time. Pedigree analysis highlighted 77 parents-offspring trios and 44 parent-offspring relationships, some of them already known and others new. Genetic-structure analysis showed a more likely number of three ancestral groups, with a high percentage of admixed samples. A structure based on the genetic background of genotypes was not observed. A core collection of 70 genotypes captured 100% of the entire number (373) of detected alleles. Most of these genotypes were unidentified or poorly characterized. The information provided in this paper could assist breeders in their efforts to exploit still unexplored individuals useful for long-term breeding plans. 

DOI:

Publication date: April 23, 2021

Issue: Enoforum 2021

Type: Article

Authors

Gabriella De Lorenzis1*, Daniele Migliaro2, Davide Bianchi1, Giovambattista Simone Di Lorenzo1, Barbara De Nardi2, Massimo Gardiman2, Osvaldo Failla1, Lucio Brancadoro1, Manna Crespan2*

Department of Agricultural and Environmental Sciences, Milano, Italy
CREA – Research Centre for Viticulture and Enology, Conegliano, Italy

Contact the author

Tags

Enoforum 2021 | IVES Conference Series

Citation

Related articles…

Impact of glutathione and elemental sulphur juice addition on the volatile thiol production in South African Sauvignon blanc wine

Three compounds, 3-mercaptohexanol (3MH), 3-mercaptohexyl-acetate (3MHA) and 4-mercapto-4-methylpentan-2-one (4MMP), also known as varietal thiols, have been identified to contribute positively to wine aroma and are responsible for the distinct gooseberry, grapefruit, guava and box tree character found in Sauvignon blanc wines. Certain volatile thiol compounds though, can cause off-aromas of onion, garlic, rubber and rotten egg, this group of molecules is known as reductive sulphur compounds (RSC). This study looks into how the addition of sulphur-compounds to Sauvignon blanc juice contributes to the varietal thiol (3MH and 3MHA) concentration and reductive sulphur compound concentration in South African Sauvignon blanc wine.

The representation of the vines: from symbol to spectacle

Landscapes such as its representation express values, beliefs and intentions of the individuals and the communities that produce them.

Mapping grapevine metabolites in response to pathogen challenge: a Mass Spectrometry Imaging approach

Every year, viticulture is facing several outbreaks caused by established diseases, such as downy mildew and grey mould, which possess different life cycles and modes of infection. To cope with these different aggressors, grapevine must recognize them and arm itself with an arsenal of defense strategies.
The regulation of secondary metabolites is one of the first reactions of plants upon pathogen challenge. Their rapid biosynthesis can highly contribute to strengthen the defense mechanisms allowing the plant to adapt, defend and survive.

Grapevine responses to red blotch disease – a structural-functional perspective of symptomatology development and fruit quality

Red Blotch disease caused by Grapevine red blotch-associated virus (GRBaV) is a severe concern to grape growers and winemakers in major grape-growing regions worldwide. One key aspect of all viruses, including Red Blotch, is their intimate association with cell components and anomalous structures following infection. Therefore, the objective of this study was to analyze symptomatology, vine function, fruit quality and ultrastructure of various tissues and document the relationship of ultrastructural cytopathology with the GRBaV infection in Pinot Noir and Merlot employing various microscopy techniques.

Effect of soil texture on early bud burst

Notre objectif est d’étudier de façon précise les relations entre la physiologie de la vigne et le sol, en prenant en compte l’effet millésime. Nous avons plus précisément étudier la précocité de débourrement de la vigne (stade D) en fonction de la texture du sol et plus particulièrement de la teneur en éléments grossiers.