Macrowine 2021
IVES 9 IVES Conference Series 9 Screening of aroma metabolites within a set of 90 Saccharomyces strains

Screening of aroma metabolites within a set of 90 Saccharomyces strains

Abstract

Currently, the main demand in the global wine market relies on products with unique flavour profiles, character, and typicity, and the metabolism of yeasts greatly influences the organoleptic properties of wines. Therefore, the natural diversity of Saccharomyces strains rises in interest over the last decade, but a large part of this phenotypic diversity remains unexplored. Moreover, the genetic basis underlying the variation in the production of flavour-active metabolites within the Saccharomyces genus remains poorly understood. The main purpose of this project is to provide a better understanding of how the synthesis of these flavour-active compounds is modulated at genetic level, aiming to identify genes with specific functions in the metabolism of yeasts. This information will be obtained through the generation of novel hybrids between different Saccharomyces species and the use of quantitative genetics. In this context, the first step was to assess the phenotypic diversity at the scale of Saccharomyces genus, regarding traits of industrial interest. With this aim, 90 yeast strains of all the eight species which compose the Saccharomyces clade were screened for their fermentative capacities and the production of aromas and other compounds of interest (such as glycerol or succinate). Fermentations in oenological conditions were carried out at different temperatures, monitoring the kinetic profiles and analysing the production of the main fermentation metabolites (by HPLC) as well as the production of more than 40 aroma compounds (by GC-MS). The sporulation ability of the strains, necessary for the hybridization, was also assessed. Important differences were found in the kinetic and volatile profiles of the strains, and the whole dataset provides a comprehensive picture of the phenotypic diversity within the genus Saccharomyces. This information confirms the interest in further development of genetic approaches to identify the molecular basis underlying the studied traits and opens the door for their improvement.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Rafael Álvarez-Rafael, Sylvie DEQUIN, Edward J. LOUIS, Carole CAMARASA

UMR 1083 Sciences Pour l’Oenologie, INRAE, Montpellier SupAgro, Montpellier, France, UMR 1083 Sciences Pour l’Oenologie, INRAE, Montpellier SupAgro, Montpellier, France ,Centre of Genetic Architecture of Complex Traits, University of Leicester, Leicester, UK, UMR 1083 Sciences Pour l’Oenologie, INRAE, Montpellier SupAgro, Montpellier, France

Contact the author

Keywords

Alcoholic fermentation; genus saccharomyces; phenotypic diversity; fermentative volatile compounds

Citation

Related articles…

Using unmanned aerial vehicle with multispectral camara to efficiently and precisely monitor the incidence of downy mildew and technical maturity of beibinghong (vitis amurensis Rpru.) grapes 

Multispectral unmanned aerial vehicle (UAV) has been successfully applied in monitoring vine vegetative growth, however, there are still potential relationships between remote sensing vegetation indexes (vis) and vine condition or grape quality, so it is worthy a deeper investigation to make a better use of UAV. One of the purposes of the study is to find out vis that could denote the severity of downy mildew (DM), so that precise and differentiated control strategies would be adopted subsequently.

Application of uv-led in wine as an alternative to sulphur dioxide

Sulfites (SO2) are commonly used in the wine industry to preserve products during storage for antiseptic and antioxidant purposes (Oliveira et al., 2011).

Sensory profile: a tool to characterize originality of wines produced without sulfites

A trend to reduce chemical inputs in wines exists, especially sulfur dioxide (SO2). This additive is widely used due to its antioxidant, antiseptic and antioxidasic properties. During without sulfites vinification, bioprotection by adding yeast on harvest could be a sulfites alternative. With extension of this wine market, sensory impact linked to sulfites absence and/or sulfites alternative should be evaluated. That’s what this approach proposes to do, focusing on sensory characteristics of wines produced with or without SO2 addition during the winemaking process. METHODS: Wines were elaborated from Merlot grapes of two maturity levels according to three modalities: SO2, without SO2 and bioprotection on harvest (mix of Torulaspora delbrueckii and Metschnikowia pulcherrima). SO2 modality was sulfited throughout the winemaking and aging processes whether other modalities received any addition. After two years of aging, sensory studies were carried out with a specific panel for one month. First, descriptors were generated to differentiate the wines, then panelists were trained on these specific descriptors for five sessions and finally wines sensory profiles were elaborated

OPTIMIZING THE IDENTIFICATION OF NEW THIOLS AT TRACE LEVEL IN AGED RED WINES USING NEW OAK WOOD FUNCTIONALISATION STRATEGY

During bottle aging, many thiol compounds are involved in the expression of bouquet of great aged red wines according to the quality of the closure.1,2 Identifying thiol compounds in red wines is a challenging task due several drawbacks including, the complexity of the matrix, the low concentration of these impact compounds and the amount of wine needed.3,4
This work aims to develop a new strategy based on the functionalisation of oak wood organic extracts with H₂S, to produce new thiols, in order to mimic what can happen in red wine during bottle aging. Following this approach and through sensory analysis experiments, we demonstrated that the vanilla-like aroma of fresh oak wood was transformed into intense “meaty” nuances similar to those found in old but non oxidized red wines.

Exploring the resistance of non-Saccharomyces wine yeasts to dehydration-rehydration processes

AIM: The use of non-Saccharomyces (NS) yeasts in multi-starter fermentations with S. cerevisiae is a trend in the wine industry, but the number of strains commercially available in a powder formulation, such as active dry yeasts (ADY), is still limited.