Macrowine 2021
IVES 9 IVES Conference Series 9 Lamp – a modern tool for the detection of fungal infections in the vineyard

Lamp – a modern tool for the detection of fungal infections in the vineyard

Abstract

AIM: Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. Its simple handling and no need for dedicated equipment together with an evaluation of the amplification event by in-tube detection make this method advantageous and economically affordable for on-site investigations in the industry. In this study, the applicability of such assays for the detection of fungal infections in grape, soil, and must samples was tested and optimized.

METHODS: 88 grape, 42 soil, and 15 must samples from different vineyards in Europe collected during the harvest 2020 were tested with LAMP assays optimized for the specific detection of Botrytis (B.) cinerea [2] responsible for Botrytis bunch rot, the gushing-inducing fungus Penicillium (P.) oxalicum [3], and with a newly developed LAMP assay for the detection of the mycotoxin-producing and gushing-inducing fungus P. expansum [4,5].

RESULTS: The optimized LAMP assay for the detection of B. cinerea revealed positive samples in all tested vineyards. For P. oxalicum, 6% of grape samples showed positive results while soil and must were tested negative. P. expansum was only found in Germany with 28% of grape, 10% of soil, and 13% of must samples revealing positive results.

CONCLUSIONS:

The application of LAMP assays for the detection of fungal infections prior to the occurrence of visual mold symptoms by testing samples from vineyards is particularly beneficial. A specific and sensitive detection can be performed within 60 minutes of incubation and results can be monitored by naked eye inspection at day light. A simple sample preparation and the use of simple equipment like a water bath make LAMP a powerful tool for on-site investigations in the winemaking industry. SUPPORT: AiF 19952 N.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Lisa M. Frisch, Magdalena A. MANN, y Rudi F. VOGEL,  Ludwig NIESSEN

Technical University of Munich, Germany

Contact the author

Keywords

loop-mediated isothermal amplification (lamp), diagnosis, fungal infection, champagne gushing, on-site investigation

Citation

Related articles…

Screening of aroma metabolites within a set of 90 Saccharomyces strains

Currently, the main demand in the global wine market relies on products with unique flavour profiles, character, and typicity, and the metabolism of yeasts greatly influences the organoleptic properties of wines. Therefore, the natural diversity of Saccharomyces strains rises in interest over the last decade, but a large part of this phenotypic diversity remains unexplored

Gas chromatography-olfactometry characterization of corvina and corvinone young and aged wines

AIM AND METHODS: Corvina and Corvinone are the two main grape varieties used in the production of Valpolicella, Recioto and Amarone, top-quality red wines in north-eastern Italy. This work aimed at determining the aroma composition of Corvina and Corvinone experimental wines and identify the main aroma compounds contributing to the aroma characteristics of Corvina and Corvinone monovarietal wines. Five Corvina and five Corvinone wines were studied, the grapes coming from five different vineyards in Valpolicella. Volatile compounds were extracted by SPE and identified and quantified by gas chromatography-mass spectrometry (GC-MS), whereas their aroma impact was determined by gas chromatography- olfactometry (GC-O).RESULTS: Based on the GC-MS-O analysis, 95 odor zones were detected, from which 68 compounds were successfully identified. Using the criterion of a value higher than 30% in modified frequency (MF %), 51 compounds were selected and grouped according to odor similarity. Compounds with values below 30% were discarded.

Subsurface irrigation: a means to reduce chemical and water inputs in vineyards

Grape growers around the world are seeking to reduce their reliance on herbicides. However, traditional alternatives to chemical weed control do not always integrate seamlessly into established vineyard operations. Employing nonchemical weed management often requires trellis alterations, purchasing or hiring new equipment, and depending on region, may significantly increase tractor passes required to reach desired level of weed control. Critical thinking and thoughtful strategies are necessary to minimize expenditures and maintain quality during the transition away from herbicides. In this trial, irrigation was installed underground in an effort to minimize water loss due to evaporation, better direct the water to the vines, and reduce weed growth in the difficult to control undervine area.

PERCEPTUAL INTERACTIONS PHENOMENA INVOLVING VARIOUS VOLATILE COMPOUND FAMILIES LINKED TO SOME FRUITY NOTES IN BORDEAUX RED WINES

Fruity notes play a key role in the consumer’s appreciation of Bordeaux red wines. If literature provides a lot of knowledge about the nature of volatile compounds involved in this fruity expression, the sensory phenomena involving these compounds in mixture still need to be explored. Considering previous sensory works about the impact of esters and some overripening compounds, the goal of this work was to study the implication of perceptual interactions involving red wine odorant compounds of diverse origins and described as potentially affecting fruity aromatic expression.

Integrated approaches for the functional characterization of miRNAs in grapevine

Micro(mi)RNAs are small non-coding RNAs that regulate several pathways and are widely recognised as key players in plant development, tissue differentiation, and many other important physiological processes, including plant adaptation to biotic and abiotic stresses. The release of plant genomes and the application of high throughput sequencing have considerably extended miRNA discovery across many species, including grapevine (Vitis spp.). Despite their relevance in plant development, functional studies in grapevine to clarify the function of miRNAs are not yet available. Through the grapevine genetic improvement platform IMPROVIT at CNR-IPSP (http://www.ipsp.cnr.it/en/thematics/turin-headquarter-thematics/improvit/), we developed integrated approaches to discover miRNA function in grapevine.