 IVES 9 IVES Conference Series 9 Lamp – a modern tool for the detection of fungal infections in the vineyard

Lamp – a modern tool for the detection of fungal infections in the vineyard

Abstract

AIM: Loop-mediated isothermal amplification (LAMP) [1] is a modern technology for fast and sensitive amplification of specific DNA sequences under isothermal conditions. Its simple handling and no need for dedicated equipment together with an evaluation of the amplification event by in-tube detection make this method advantageous and economically affordable for on-site investigations in the industry. In this study, the applicability of such assays for the detection of fungal infections in grape, soil, and must samples was tested and optimized.

METHODS: 88 grape, 42 soil, and 15 must samples from different vineyards in Europe collected during the harvest 2020 were tested with LAMP assays optimized for the specific detection of Botrytis (B.) cinerea [2] responsible for Botrytis bunch rot, the gushing-inducing fungus Penicillium (P.) oxalicum [3], and with a newly developed LAMP assay for the detection of the mycotoxin-producing and gushing-inducing fungus P. expansum [4,5].

RESULTS: The optimized LAMP assay for the detection of B. cinerea revealed positive samples in all tested vineyards. For P. oxalicum, 6% of grape samples showed positive results while soil and must were tested negative. P. expansum was only found in Germany with 28% of grape, 10% of soil, and 13% of must samples revealing positive results.

CONCLUSIONS:

The application of LAMP assays for the detection of fungal infections prior to the occurrence of visual mold symptoms by testing samples from vineyards is particularly beneficial. A specific and sensitive detection can be performed within 60 minutes of incubation and results can be monitored by naked eye inspection at day light. A simple sample preparation and the use of simple equipment like a water bath make LAMP a powerful tool for on-site investigations in the winemaking industry. SUPPORT: AiF 19952 N.

DOI:

Publication date: September 3, 2021

Issue: Macrowine 2021

Type: Article

Authors

Lisa M. Frisch, Magdalena A. MANN, y Rudi F. VOGEL, Ludwig NIESSEN

Technical University of Munich, Germany

Contact the author

Keywords

loop-mediated isothermal amplification (lamp), diagnosis, fungal infection, champagne gushing, on-site investigation

Citation

Training system and its influence on iso-anisohydric behavior of cv. Syrah

Water use efficiency is one of the most valued objectives in vine growing in mediterranean climates (de la fuente et al., 2015). Due to this, the grape growers provide different adaptation strategies according to their efficient consumption against the presumable water deficit generated under these environmental conditions. The use of non-positioned shoot systems (like sprawl, bush, etc.) Can help to achieve this objective.

Different soil types and relief influence the quality of Merlot grapes in a relatively small area in the Vipava Valley (Slovenia) in relation to the vine water status

Besides location and microclimatic conditions, soil plays an important role in the quality of grapes and wine. Soil properties influence…

A methyl salicylate glycoside mapping of monovarietal Italian white wines.

Among the main plant secondary metabolites, glycosides have a key-role in wine chemistry. Glycosides are non-volatile complex composed of a non-sugar component (aglycone) bound to one or more carbohydrates.

Effect of multi-level and multi-scale spectral data source on vineyard state assessment

Currently, the main goal of agriculture is to promote the resilience of agricultural systems in a sustainable way through the improvement of use efficiency of farm resources, increasing crop yield and quality under climate change conditions. This last is expected to drastically modify plant growth, with possible negative effects, especially in arid and semi-arid regions of Europe on the viticultural sector. In this context, the monitoring of spatial behavior of grapevine during the growing season represents an opportunity to improve the plant management, winegrowers’ incomes, and to preserve the environmental health, but it has additional costs for the farmer. Nowadays, UAS equipped with a VIS-NIR multispectral camera (blue, green, red, red-edge, and NIR) represents a good and relatively cheap solution to assess plant status spatial information (by means of a limited set of spectral vegetation indices), representing important support in precision agriculture management during the growing season. While differences between UAS-based multispectral imagery and point-based spectroscopy are well discussed in the literature, their impact on plant status estimation by vegetation indices is not completely investigated in depth. The aim of this study was to assess the performance level of UAS-based multispectral (5 bands across 450-800nm spectral region with a spatial resolution of 5cm) imagery, reconstructed high-resolution satellite (Sentinel-2A) multispectral imagery (13 bands across 400-2500 nm with spatial resolution of <2 m) through Convolutional Neural Network (CNN) approach, and point-based field spectroscopy (collecting 600 wavelengths across 400-1000 nm spectral region with a surface footprint of 1-2 cm) in a plant status estimation application, and then, using Bayesian regularization artificial neural network for leaf chlorophyll content (LCC) and plant water status (LWP) prediction. The test site is a Greco vineyard of southern Italy, where detailed and precise records on soil and atmosphere systems, in-vivo plant monitoring of eco-physiological parameters have been conducted.

Influence of polysaccharide extracts from wine by-products on the volatile composition of sparkling white wines

In the production of sparkling wines, during the second fermentation, mannoproteins are released by yeast autolysis, which affect the quality of the wines. The effect of mannoproteins has been extensively studied, and may affect aroma and foam quality. However, there are no studies on the effect of other polysaccharides such as those from grapes. Considering the large production of waste from the wine industry, it was proposed to obtain polysaccharide-rich extracts from some of these by-products[1].