Macrowine 2021
IVES 9 IVES Conference Series 9 Development of a new commercial phenolic analysis method for red grapes

Development of a new commercial phenolic analysis method for red grapes

Abstract

Grape phenolic content is an important quality factor that influences the appearance and mouthfeel of premium red wines. The wine industry uses a variety of commercial phenolic assays to determine phenolic maturity in red grapes. Some of these assays, however, are dated more than 20 years ago and do not always accurately reflect wine phenolic content from grape phenolic extracts. The aim of this study is to develop an adjusted phenolic sample preparation and extraction protocol so it can be used in commercial wineries and/or laboratories.In this study, six grape cultivars (Pinotage, Cabernet-Sauvignon, Merlot, Shiraz, Cinsualt and Pertit Verdot) were collected from 42 different vineyards from across 15 different farms. Representative samples were taken from the grapes of each block. Grape extractions were done in duplicates using four different methods namely Glories, Iland, Modified Iland and a custom made Machine crushed method. The Glories, Iland and modified Iland methods produces homogenized grapes, while the machine crushed method uses grape samples where only the skins were crushed. The modified Iland and machine crushed extraction methods were exposed to microwave treatment and extracted in a 50% alcohol solution for 30 min and 1 hour and 3h, 24h and 40h, respectively.Wines were made from every grape samples. Phenolic analyses were done for anthocyanins, tannins, total phenols index and colour density on the grapes and wines. Variation in the phenolic composition of the grapes where the different extraction methods were observed. Correlations between grapes and wines phenolic data with the different grape extraction methods will also be shown.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Asiphe Makalisa

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University,Kiera Lambrecht, South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University Jose Luis Aleixandre Tudo, Universitat Politecnica de Valencia, Instituto de Ingenieria de Alimentos para el Desarrollo (IIAD), Departamento de Tecnología de Alimentos and Stellenbosch University, South African Grape and Wine Research Institute (SAGWRI), Department of Viticulture and Oenology Keren Bindon, Australian Grape and Wine Research Institute, Adelaide Wessel du Toit, South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University

Contact the author

Keywords

extraction, phenolic assay, red grapes, tannins

Citation

Related articles…

Nematode vectors, grape fanleaf virus (GFLV) incidence and free virus vine plants obtaining in “Condado de Huelva” vineyards zone

The « Condado de Huelva » Registered Appellation Origin Mark (RAOM) is located in the Province of Huelva, in the southwest of Andalucía (Spain), being limited by the Atlantic Ocean and the Province of Sevilla. « Zalema », a white high productive grapevine plant is its major cultivar. The predominant rootstocks used are « Rupestris du Lot », « Castel 196-17 », « Couderc 161-49 », Couderc 33-09 », « Richter 110 » and « Millardet 41-B ». Traditionally, « Zalema » cv. has been dedicated to the elaboration of amber, bouquet-flavoured wines and in the last years mainly to young, fruit-flavoured white table wines.

New oenological technology for adaptation to climate change: reduction of alcohol content during wine fermentation through stripping, with fermentative CO2

The capture and valorization of fermentative CO2 have been developed for several years by the company w platform for internal uses, notably in the cellars: inerting, cooling, reduction of water consumption, extraction, with aroma valorization. In a context of climatic warming during the vegetative cycle, grapes are currently harvested with a significant sugar concentration, a phenomenon that is expected to intensify in the coming decades. The high alcohol content of the resulting wines goes against the demand of customers who are seeking high-quality wines with less alcohol.

Comparison of the free radical-scavenging activity in infected oidium and sound dolcetto grape cultivar grown in a terroir of Central Italy

The importance of polyphenols, which are present in many vegetables and grapes too, is well-know and documented. Specific research works about the red grape

Impact of technical itineraries on the diversity and the functioning of arbuscular mycorrhizal fungi and associated microorganisms in vineyards soils and grapevine roots

Context and purpose. The vine is a holobiont, where the plant interacts positively, negatively, and neutrally with microbes that together form the vine’s microbiome.

Application of non-Saccharomyces yeasts in peculiar winemaking, sparkling and sweet wines: biological acidification, prise de mousse, aroma profile. Two cases of study

In this video recording of the IVES science meeting 2025, Raffaele Guzzon (Fondazione Edmund Mach, Centro di Trasferimento Tecnologico, San Michele all’Adige (TN), Italy) speaks about the application of non-Saccharomyces yeasts in peculiar winemaking, sparkling and sweet wines (biological acidification, prise de mousse, aroma profile). This presentation is based on an original article accessible for free on OENO One.