Macrowine 2021
IVES 9 IVES Conference Series 9 Development of a new commercial phenolic analysis method for red grapes

Development of a new commercial phenolic analysis method for red grapes

Abstract

Grape phenolic content is an important quality factor that influences the appearance and mouthfeel of premium red wines. The wine industry uses a variety of commercial phenolic assays to determine phenolic maturity in red grapes. Some of these assays, however, are dated more than 20 years ago and do not always accurately reflect wine phenolic content from grape phenolic extracts. The aim of this study is to develop an adjusted phenolic sample preparation and extraction protocol so it can be used in commercial wineries and/or laboratories.In this study, six grape cultivars (Pinotage, Cabernet-Sauvignon, Merlot, Shiraz, Cinsualt and Pertit Verdot) were collected from 42 different vineyards from across 15 different farms. Representative samples were taken from the grapes of each block. Grape extractions were done in duplicates using four different methods namely Glories, Iland, Modified Iland and a custom made Machine crushed method. The Glories, Iland and modified Iland methods produces homogenized grapes, while the machine crushed method uses grape samples where only the skins were crushed. The modified Iland and machine crushed extraction methods were exposed to microwave treatment and extracted in a 50% alcohol solution for 30 min and 1 hour and 3h, 24h and 40h, respectively.Wines were made from every grape samples. Phenolic analyses were done for anthocyanins, tannins, total phenols index and colour density on the grapes and wines. Variation in the phenolic composition of the grapes where the different extraction methods were observed. Correlations between grapes and wines phenolic data with the different grape extraction methods will also be shown.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Asiphe Makalisa

South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University,Kiera Lambrecht, South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University Jose Luis Aleixandre Tudo, Universitat Politecnica de Valencia, Instituto de Ingenieria de Alimentos para el Desarrollo (IIAD), Departamento de Tecnología de Alimentos and Stellenbosch University, South African Grape and Wine Research Institute (SAGWRI), Department of Viticulture and Oenology Keren Bindon, Australian Grape and Wine Research Institute, Adelaide Wessel du Toit, South African Grape and Wine Research Institute, Department of Viticulture and Oenology, Stellenbosch University

Contact the author

Keywords

extraction, phenolic assay, red grapes, tannins

Citation

Related articles…

Salubrity of environment and zoning process: first consideration on the radioactivity of vineyard soils

La salubrité du milieu et des aliments intervient de plus en plus lourdement, et souvent négativement, sur la santé de l’homme, aussi bien sur l’individu que sur la société tout entière.

Which microorganisms contribute to mousy off-flavour in our wines?

In this video recording of the IVES science meeting 2024, Mariona H Gil i Cortiella (Universidad Autónoma de Chile, Santiago de Chile, Chile) speaks about Which microorganisms contribute to mousy off-flavour in owines. This presentation is based on articles accessible for free on OENO One and IVES Technical Reviews.

Exploring zoxamide sensitivity in Plasmopara viticola populations: implications for fungicide management in precision agriculture

Fungicides play a critical role in managing grapevine downy mildew caused by the oomycete Plasmopara viticola, a biotrophic and polycyclic pathogen with a high risk of fungicide resistance. Zoxamide, categorized as a low to medium resistance risk, disrupts cell division by inhibiting tubulin polymerization. Resistance to zoxamide is uncommon in field isolates. This six-year study (2017-2022) aimed to detect and quantify zoxamide sensitivity in P. viticola populations across varying resistance pressures in Italian grapevine regions. Analysis of 126 samples from 57 vineyards, mainly in North-Eastern Italy, revealed that most samples exhibited EC50, EC95, and MIC values below 0.1 and 10 mg/L of zoxamide, respectively. Nineteen vineyards showed reduced sensitivity (MIC>100 mg/L), but only four samples were characterized by 24-54% resistant oospores at >100 mg/L of zoxamide.

Utilizing ozone for the management of powdery mildew (Erysiphe necator Schwein.) in vineyards: potential and challenges

Powdery mildew, caused by fungal pathogens, poses a significant threat to grapevines in the DOCa Rioja region. In efforts to improve control strategies while reducing reliance on conventional phytosanitary products, ozone could constitute a potential alternative. However, it has short persistence, thus requiring frequent treatments. This study aimed to assess the suitability of ozone as an active substance for controlling powdery mildew within a phytosanitary strategy aimed at reducing conventional phytosanitary product usage. The strategy integrating ozone with conventional products yielded powdery mildew levels comparable to conventional treatments in both disease incidence and severity.

Influence of pre-harvest elicitors treatment during ripening period on phenolic composition in Monastrell grapes

Phenolic compounds are very importants in crop plants, which is why there have been the subject of a large number of studies