Macrowine 2021
IVES 9 IVES Conference Series 9 The anthocyanin profile of galician endangered varieties. A tool for varietal selection

The anthocyanin profile of galician endangered varieties. A tool for varietal selection

Abstract

AIM: The current loss of genetic grapevine diversity is mainly due to the reduced number of varieties used for making wine. A way of preserved endangered varieties is the establishment of germplasm banks. The anthocyanin profile is a key factor in determining the oenological potential of red wine grape varieties. Thus, this work analyses the anthocyanin profiles of 29 varieties from the germplasm bank located in ¨Estación de Viticultura y Enología de Galicia¨ (EVEGA), Ourense (Galicia, Northwest Spain) in 2018 and 2019 seasons. 

METHODS: At harvest, grapes were picked up and the anthocyanin substances were extracted and analyzed by HPLC (1, 2). Results were subjected to statistical analysis, ANOVA (factor variety) and Principal Component Analysis (PCA).

RESULTS: The anthocyanins were identified as the monoglucoside (GLU) acetylglucoside (AC) and p-coumaroylglucoside forms (CM) of cyanidin (Cy), delphinidin (Dp), malvidin (Mv), pelargonidin (Pel), peonidin (Pn) and petunidin (Pt). Sixteen compounds were identified and quantified (mg/Kg of berry fresh weight, FW). Significant diferences between varieties (p<0.001) were found for the biannual means of all compounds. MvG had the highest content in all varieties analyzed, with the exception of Zamarrica, Xafardán, Moscatel de Hamburgo and Brancellao. Respect to the rest of varieties, Sousón, Castañal, Ferrón, Espadeiro and Caíño Bravo (1832, 1323, 1327, 1173 and 1097 mg/Kg FW respectively) showed the highest contents of Total Anthocyanin. It is worth noting that these varieties belong to the same genetic population (3).These contents were higher than those found in Mencia (643 mg/Kg FW) and Tempranillo (891 mg/Kg FW), varieties widely cultivated in Galicia and Spain respectively.

Application of Principal Component Analysis (PCA) to experimental data showed a good separation of varieties according to the anthocyanin profile. (71.32 and 74.22 % of the total variance in 2018 and 2019 respectively). PCA also showed a group including Ferrón, Sousón, Castañal and Espadeiro varieties related to high contents of ΣGLU, ΣDel, ΣPet and ΣMal

CONCLUSION

 Results demonstrated a high degree of anthocyanin profile difference between the varieties analyzed. Due to their anthocyanidin profile some of these varieties could play an important role in the red winery industry.

 

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Ángela Díaz Fernández

Viticulture and Oenology Station of Galicia [EVEGA] (Ourense, Galicia- Spain), Viticulture and Oenology Station of Galicia (EVEGA), Ourense (Galicia)  2 Technological Agri-Food Institute of Extremadura (CICYTEX, INTAEX), Badajoz (Extremadura).

Contact the author

Keywords

Vitis vinifera, red grapevine, germplasm bank, anthocyanidine

Citation

Related articles…

Adaptation to soil and climate through the choice of plant material

Choosing the rootstock, the scion variety and the training system best suited to the local soil and climate are the key elements for an economically sustainable production of wine. The choice of the rootstock/scion variety best adapted to the characteristics of the soil is essential but, by changing climatic conditions, ongoing climate change disrupts the fine-tuned local equilibrium. Higher temperatures induce shifts in developmental stages, with on the one hand increasing fears of spring frost damages and, on the other hand, ripening during the warmest periods in summer. Expected higher water demand and longer and more frequent drought events are also major concerns. The genetic control of the phenotypes, by genomic information but also by the epigenetic control of gene expression, offers a lot of opportunities for adapting the plant material to the future. For complex traits, genomic selection is also a promising method for predicting phenotypes. However, ecophysiological modelling is necessary to better anticipate the phenotypes in unexplored climatic conditions Genetic approaches applied on parameters of ecophysiological models rather than raw observed data are more than ever the basis for finding, or building, the ideal varieties of the future.

The concept of terroir: what place for microbiota?

Microbes play key roles on crop nutrient availability via biogeochemical cycles, rhizosphere interactions with roots as well as on plant growth and health. Recent advances in technologies, such as High Throughput Sequencing Techniques, allowed to gain deeper insight on the structure of bacterial and fungal communities associated with soil, rhizosphere and plant phyllosphere. Over the past 10 years, numerous scientific studies have been carried out on the microbial component of the vineyard. Whether the soil or grape compartments have been taken into account, many studies agree on the evidence of regional delineations of microbial communities, that may contribute to regional wine characteristics and typicity. Some authors proposed the term “microbial terroir” including “yeast terroir” for grapes to describe the connection between microbial biogeography and regional wine characteristics. Many factors are involved in terroir including climate, soil, cultivar and human practices as well as their interactions. Studies considering “microbial terroir” greatly contributed to improve our knowledge on factors that shape the vineyard microbial structure and diversity. However, the potential impact of “microbial terroir” on wine composition has yet not received strong scientific evidence and many questions remain to be addressed, related to the functional characterization of the microbial community and its impact on plant physiology and grape composition, the origins and interannual stability of vineyard microbiota, as well as their impact on wine sensorial attributes. The presentation will give an overview on the role of microbiota as a terroir component and will highlight future perspectives and challenges on this key subject for the wine industry.

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

Influence of weather and climatic conditions on the viticultural production in Croatia

The research includes an analysis of the impact of weather conditions on phenological development of the vine and grape quality, through monitoring of four experimental cultivars (Chardonnay, Graševina, Merlot and Plavac mali) over two production years. In each experimental vineyard, which were evenly distributed throughout the regions of Slavonia and The Croatian Danube, Croatian Uplands,

Geospatial trends of bioclimatic indexes in the topographically complex region of Barolo DOCG

Barolo DOCG is an economically important wine producing region in Northwest Italy. It is a small region of approximately 70 km2 gross area. The topography is very complex with steep sloped hills ranging in elevation from below 200 m to 550 m. Barolo DOCG wine is made exclusively from the Nebbiolo grape. Bioclimatic indexes are often used in viticulture to gain a better understanding of broader climate trends which can be compared temporally and geographically. These indexes are also used for identifying potential phenological timing, growing region suitability, and potential risks associated with expected climatic changes. Understanding how topography influences bioclimatic indexes can help with understanding of mesoscale climate behaviour leading to improved decision making and risk management strategies. The average monthly maximum and minimum temperatures, the Cool Night Index, the Huglin Index, and the monthly diurnal range (from July to October) were calculated using data from 45 weather stations within a 40 km radius of the Barolo DOCG growing area between the years 1996 and 2019. Linear and multiple regression models were developed using independent variables (elevation, aspect, slope) extracted from a digital elevation model to identify significant relationships. Bioclimatic indexes were then kriged with external drift using independent variables that showed significant relationships with the bioclimatic index using a 100 m resolution grid. The maximum monthly temperatures and the Huglin Index showed consistent significant negative relationships with elevation in all years. The minimum monthly temperatures showed no relationship with elevation but in some months a small but significant relationship was observed with aspect. Due to the lack of a relationship between minimum monthly temperatures and elevation compared to the significant relationship between maximum monthly temperatures and elevation, monthly diurnal range had a negative relationship with elevation.