Macrowine 2021
IVES 9 IVES Conference Series 9 The anthocyanin profile of galician endangered varieties. A tool for varietal selection

The anthocyanin profile of galician endangered varieties. A tool for varietal selection

Abstract

AIM: The current loss of genetic grapevine diversity is mainly due to the reduced number of varieties used for making wine. A way of preserved endangered varieties is the establishment of germplasm banks. The anthocyanin profile is a key factor in determining the oenological potential of red wine grape varieties. Thus, this work analyses the anthocyanin profiles of 29 varieties from the germplasm bank located in ¨Estación de Viticultura y Enología de Galicia¨ (EVEGA), Ourense (Galicia, Northwest Spain) in 2018 and 2019 seasons. 

METHODS: At harvest, grapes were picked up and the anthocyanin substances were extracted and analyzed by HPLC (1, 2). Results were subjected to statistical analysis, ANOVA (factor variety) and Principal Component Analysis (PCA).

RESULTS: The anthocyanins were identified as the monoglucoside (GLU) acetylglucoside (AC) and p-coumaroylglucoside forms (CM) of cyanidin (Cy), delphinidin (Dp), malvidin (Mv), pelargonidin (Pel), peonidin (Pn) and petunidin (Pt). Sixteen compounds were identified and quantified (mg/Kg of berry fresh weight, FW). Significant diferences between varieties (p<0.001) were found for the biannual means of all compounds. MvG had the highest content in all varieties analyzed, with the exception of Zamarrica, Xafardán, Moscatel de Hamburgo and Brancellao. Respect to the rest of varieties, Sousón, Castañal, Ferrón, Espadeiro and Caíño Bravo (1832, 1323, 1327, 1173 and 1097 mg/Kg FW respectively) showed the highest contents of Total Anthocyanin. It is worth noting that these varieties belong to the same genetic population (3).These contents were higher than those found in Mencia (643 mg/Kg FW) and Tempranillo (891 mg/Kg FW), varieties widely cultivated in Galicia and Spain respectively.

Application of Principal Component Analysis (PCA) to experimental data showed a good separation of varieties according to the anthocyanin profile. (71.32 and 74.22 % of the total variance in 2018 and 2019 respectively). PCA also showed a group including Ferrón, Sousón, Castañal and Espadeiro varieties related to high contents of ΣGLU, ΣDel, ΣPet and ΣMal

CONCLUSION

 Results demonstrated a high degree of anthocyanin profile difference between the varieties analyzed. Due to their anthocyanidin profile some of these varieties could play an important role in the red winery industry.

 

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Ángela Díaz Fernández

Viticulture and Oenology Station of Galicia [EVEGA] (Ourense, Galicia- Spain), Viticulture and Oenology Station of Galicia (EVEGA), Ourense (Galicia)  2 Technological Agri-Food Institute of Extremadura (CICYTEX, INTAEX), Badajoz (Extremadura).

Contact the author

Keywords

Vitis vinifera, red grapevine, germplasm bank, anthocyanidine

Citation

Related articles…

Permanent cover cropping with reduced tillage increased resiliency of wine grape vineyards to climate change

Majority of California’s vineyards rely on supplemental irrigation to overcome abiotic stressors. In the context of climate change, increases in growing season temperatures and crop evapotranspiration pose a risk to adaptation of viticulture to climate change. Vineyard cover crops may mitigate soil erosion and preserve water resources; but there is a lack of information on how they contribute to vineyard resiliency under tillage systems. The aim of this study was to identify the optimum combination of cover crop sand tillage without adversely affecting productivity while preserving plant water status. Two experiments in two contrasting climatic regions were conducted with two cover crops, including a permanent short stature grass (P. bulbosa hybrid), barley (Hordeum spp), and resident vegetation under till vs. no-till systems in a Ruby Cabernet (V. vinifera spp.) (Fresno) and a Cabernet Sauvingon (Napa) vineyard. Results indicated that permanent grass under no-till preserved plant available water until E-L stage 17. Consequently, net carbon assimilation of the permanent grass under no-till system was enhanced compared to those with barley and resident vegetation. On the other hand, the barley under no-till system reduced grapevine net carbon assimilation during berry ripening that led to lower content of nonstructural carbohydrates in shoots at dormancy. Components of yield and berry composition including flavonoid profile at either site were not adversely affected by factors studied. Switching to a permanent cover crop under a no-till system also provided a 9% and 3% benefit in cultural practices costs in Fresno and Napa, respectively. The results of this work provides fundamental information to growers in preserving resiliency of vineyard systems in hot and warm climate regions under context of climate change.

Mesoclimate impact on Tannat in the Atlantic terroir of Uruguay

The study of climate is relevant as an element conditioning the typicity of a product, its quality and sustainability over the years. The grapevine development and growth and the final grape and wine composition are closely related to temperature, while climate components vary at mesoscale according to topography and/or proximity to large bodies of water. The objective of this work is to assess the mesoclimate of the Atlantic region of Uruguay and to determine the effect of topography and the ocean on temperature and consequently on Tannat grapevine behavior.

How can historical cultivars mitigate the effects of climate change?

IFV, INRAe and the national network “Partenaires de la Sélection Vigne” representing 37 organizations from the different wine regions, have been working increasingly closely over the last 2 decades towards the preservation of the French varietal patrimony. There are approximately 600 patrimonial varieties according to INRAe and SupAgro Montpellier experts, including ancient cultivars (400) and intravarietal crossbreeds obtained since the 19th century. In the context of a drastic reduction in such varieties from the mid 1980’s in favor of mainstream varieties, it was essential to carry out an inventory of old vines and vineyards. INRAe Vassal collection plays a key role here as it holds the largest diversity available, along with a rich bibliography and herbariums, offering us the opportunity to document and double check the identity of a cultivar, consolidating the expertise of ampelographers. The work is carried out in several stages, from verifying the existence of a variety in a small region, through to rehabilitation. During this session, the authors present the process that leads to the official registration of a variety. After this, IFV selection center takes over to initiate the process of selection and propagation. A specific focus within regions such as the Alps, Champagne and the South-West will provide details of the full procedure. Bia, Bouysselet, Chardonnay rose, Mecle and the aptly named Tardif, are some of the cultivars that have followed this procedure. Furthermore, a recent regulation established by INAO on “varieties of interest for adaptation purposes” might boost uptake by growers. Since 2006, 36 historical cultivars have been registered. Most of these have been neglected in the past due to late maturity, lack of sugar and high titratable acidity at harvest time. Such characteristics are today considered as positive qualities, not only in mitigation of the effects of climate change, but also as an opportunity for restoring diversity…

Modeling the suitability of Pinot Noir in Oregon’s Willamette Valley in a changing climate

Air temperature is the key driver of grapevine phenology and a significant environmental factor impacting yield and quality for a winegrape growing region. In this study the optimal downscaled CMIP5 ensemble for computing thegrowing season average temperature (GST) viticulture climate classification index was determined to spatially compute on a decadal basis predictions of the GST climate index and the grapevine sugar ripeness (GSR) model for Pinot Noir throughout the Willamette Valley (WV) American Viticultural Area (AVA). Forecasts for average temperature and a 220 g/L target sugar concentration level were computed using daily Localized Constructed Analogs (LOCA) downscaled CMIP5 historic and Representative Concentration Pathways (RCP) future climate projections of minimum and maximum daily temperature. We explore spatiotemporal trends of the GST climate classification index and Pinot Noir specific applications of the GSR phenology model for the WV AVA. Spatiotemporal computations of the GST climate index and Pinot Noir specific applications of the GSR model enable the opportunity to explore relationships between their computed values with one intent being to provide updated GST ranges that better align with current temperature-based modeling understanding of Pinot Noir grapevine phenology and the viticultural application of LOCA CMIP5 climate projections for the WV AVA. The Pinot Noir specific applications of the GSR model or the GST index with updated bounds indicate that the percent of the WV AVA area suitable for Pinot Noir production is currently at or near its peak value in the upper 80s to lower 90s of this century.

Late frost protection in Champagne

Probably one of the most counterintuitive impacts of climate change on vine is the increased frequency of late frost. Champagne, due to its septentrional position is historically and regularly affected by this meteorological hazard. Champagne has therefore developed a strong experience in frost protection with first experiments dating from the end of 19th century. Frost protection can be divided in two parts: passive and active. Passive protection includes all the methods that do not seek to modify the vine’s environment or resistance at the time of frost. The most iconic passive protection in Champagne is the establishment of the individual reserve. This reserve allows to stock a certain quantity of clear wine during a surplus year to compensate a meteorological hazard like frost during the following years. Other common passive methods are the control of planting area (walls, bushes, topography), the choice of grape variety, late pruning, or the impact of grass cover and tillage. Active frost protection is also divided in two parts. Most of the existing techniques tend to modify vine’s environment. Most of the time they provide warmth (candles, heaters, windmills, heating cables…), or stabilise bud’s temperature above a lethal threshold (water sprinkling). The other way to actively fight is to enhance the resistance of buds to frost (elicitors). The Comité Champagne evaluates frost protection methods following three main axes: the efficiency, the profitability, and the environmental impact through a lifecycle assessment. This study will present the results on both passive and active protection following these three axes.