Macrowine 2021
IVES 9 IVES Conference Series 9 The anthocyanin profile of galician endangered varieties. A tool for varietal selection

The anthocyanin profile of galician endangered varieties. A tool for varietal selection

Abstract

AIM: The current loss of genetic grapevine diversity is mainly due to the reduced number of varieties used for making wine. A way of preserved endangered varieties is the establishment of germplasm banks. The anthocyanin profile is a key factor in determining the oenological potential of red wine grape varieties. Thus, this work analyses the anthocyanin profiles of 29 varieties from the germplasm bank located in ¨Estación de Viticultura y Enología de Galicia¨ (EVEGA), Ourense (Galicia, Northwest Spain) in 2018 and 2019 seasons. 

METHODS: At harvest, grapes were picked up and the anthocyanin substances were extracted and analyzed by HPLC (1, 2). Results were subjected to statistical analysis, ANOVA (factor variety) and Principal Component Analysis (PCA).

RESULTS: The anthocyanins were identified as the monoglucoside (GLU) acetylglucoside (AC) and p-coumaroylglucoside forms (CM) of cyanidin (Cy), delphinidin (Dp), malvidin (Mv), pelargonidin (Pel), peonidin (Pn) and petunidin (Pt). Sixteen compounds were identified and quantified (mg/Kg of berry fresh weight, FW). Significant diferences between varieties (p<0.001) were found for the biannual means of all compounds. MvG had the highest content in all varieties analyzed, with the exception of Zamarrica, Xafardán, Moscatel de Hamburgo and Brancellao. Respect to the rest of varieties, Sousón, Castañal, Ferrón, Espadeiro and Caíño Bravo (1832, 1323, 1327, 1173 and 1097 mg/Kg FW respectively) showed the highest contents of Total Anthocyanin. It is worth noting that these varieties belong to the same genetic population (3).These contents were higher than those found in Mencia (643 mg/Kg FW) and Tempranillo (891 mg/Kg FW), varieties widely cultivated in Galicia and Spain respectively.

Application of Principal Component Analysis (PCA) to experimental data showed a good separation of varieties according to the anthocyanin profile. (71.32 and 74.22 % of the total variance in 2018 and 2019 respectively). PCA also showed a group including Ferrón, Sousón, Castañal and Espadeiro varieties related to high contents of ΣGLU, ΣDel, ΣPet and ΣMal

CONCLUSION

 Results demonstrated a high degree of anthocyanin profile difference between the varieties analyzed. Due to their anthocyanidin profile some of these varieties could play an important role in the red winery industry.

 

DOI:

Publication date: September 10, 2021

Issue: Macrowine 2021

Type: Article

Authors

Ángela Díaz Fernández

Viticulture and Oenology Station of Galicia [EVEGA] (Ourense, Galicia- Spain), Viticulture and Oenology Station of Galicia (EVEGA), Ourense (Galicia)  2 Technological Agri-Food Institute of Extremadura (CICYTEX, INTAEX), Badajoz (Extremadura).

Contact the author

Keywords

Vitis vinifera, red grapevine, germplasm bank, anthocyanidine

Citation

Related articles…

Grapevine xylem embolism resistance spectrum reveals which varieties have a lower mortality risk in a future dry climate

Wine growing regions have recently faced intense and frequent droughts that have led to substantial economical losses, and the maintenance of grapevine productivity under warmer and drier climate will rely notably on planting drought-resistant cultivars. Given that plant growth and yield depend on water transport efficiency and maintenance of photosynthesis, thus on the preservation of the vascular system integrity during drought, a better understanding of drought-related hydraulic traits that have a significant impact on physiological processes is urgently needed. We have worked towards this end by assessing vulnerability to xylem embolism in 30 grapevine commercial varieties encompassing red and white Vitis vinifera varieties, hybrid varieties characterized by a polygenic resistance for powdery and downy mildew, and commonly used rootstocks. These analyses further allowed a global assessment of wine regions with respect to their varietal diversity and resulting vulnerability to stem embolism. Hybrid cultivars displayed the highest vulnerability to embolism, while rootstocks showed the greatest resistance. Significant variability also arose among Vitis vinifera varieties, with Ψ12 and Ψ50 values ranging from -0.4 to -2.7 MPa and from -1.8 to -3.4 MPa, respectively. Cabernet franc, Chardonnay and Ugni blanc featured among the most vulnerable varieties while Pinot noir, Merlot and Cabernet Sauvignon ranked among the most resistant. In consequence, wine regions bearing a significant proportion of vulnerable varieties, such as Poitou-Charentes, France and Marlborough, New Zealand, turned out to be at greater risk under drought. These results highlight that grapevine varieties may not respond equally to warmer and drier conditions, outlining the importance to consider hydraulic traits associated with plant drought tolerance into breeding programmes and modeling simulations of grapevine yield maintenance under severe drought. They finally represent a step forward to advise the wine industry about which varieties and regions would have the lowest risk of drought-induced mortality under climate change.

Heatwaves and grapevine yield in the Douro region, crop model simulations

Heatwaves or extreme heat events can be particularly harmful to agriculture. Grapevines grown in the Douro winemaking region are particularly exposed to this threat, due to the specificities of the already warm and dry climatic conditions. Furthermore, climate change simulations point to an increase in the frequency of occurrence of these extreme heat events, therefore posing a major challenge to winegrowers in the Mediterranean type climates. The current study focuses on the application of the STICS crop model to assess the potential impacts of heatwaves in grapevine yields over the Douro valley winemaking region. For this purpose, STICS was applied to grapevines using high-resolution weather, soil and terrain datasets over the Douro. To assess the impact of heatwaves, the weather dataset (1989-2005) was artificially modified, generating periods with anomalously high temperatures (+5 ºC), at certain onset dates and with specific durations (from 5 to 9 days). The model was run with this modified weather dataset and results were compared to the original unmodified runs. The results show that heatwaves can have a very strong impact on grapevine yields, strongly depending on the onset dates and duration of the heatwaves. The highest negative impacts may result in a decrease in the yield by up to -35% in some regions. Despite some uncertainties inherent to the current modelling assessment, the present study highlights the negative impacts of heatwaves on viticultural yields in the Douro region, which is critical information for stakeholders within the winemaking sector for planning suitable adaptation measures.

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Aromatic maturity is a cornerstone of terroir expression in red wine

Harvesting grapes at adequate maturity is key to the production of high-quality red wines. Enologists and wine makers define several types of maturity, including technical maturity, phenolic maturity and aromatic maturity. Technical maturity and phenolic maturity are relatively well documented in the scientific literature, while articles on aromatic maturity are scarcer. This is surprising, because aromatic maturity is, without a doubt, the most important of the three in determining wine quality and typicity (including terroir expression). Optimal terroir expression can be obtained when the different types of maturity are reached at the same time, or within a short time frame. This is more likely to occur when the ripening takes place under mild temperatures, neither too cool, nor too hot. Aromatic expression in wine can be driven, from low to high maturity, by green, herbal, fresh fruit, ripe fruit, jammy fruit, candied fruit or cooked fruit aromas. Green and cooked fruit aromas are not desirable in red wines, while the levels of other aromatic compounds contribute to the typicity of the wine in relation to its origin. Wines produced in cool climates, or on cool soils in temperate climates, are likely to express herbal or fresh fruit aromas; while wines produced under warm climates, or on warm soils in temperate climates, may express ripe fruit, jammy fruit or candied fruit aromas. Growers can optimize terroir expression through their choice of grapevine variety. Early ripening varieties perform better in cool climates and late ripening varieties in warm climates. Additionally, maturity can be advanced or delayed by different canopy management practices or training systems.

Climate and the evolving mix of grape varieties in Australia’s wine regions

The purpose of this study is to examine the changing mix of winegrape varieties in Australia so as to address the question: In the light of key climate indicators and predictions of further climate change, how appropriate are the grape varieties currently planted in Australia’s wine regions? To achieve this, regions are classified into zones according to each region’s climate variables, particularly average growing season temperature (GST), leaving aside within-region variations in climates. Five different climatic classifications are reported. Using projections of GSTs for the mid- and late 21st century, the extent to which each region is projected to move from its current zone classification to a warmer one is reported. Also shown is the changing proportion of each of 21 key varieties grown in a GST zone considered to be optimal for premium winegrape production. Together these indicators strengthen earlier suggestions that the mix of varieties may be currently less than ideal in many Australian wine regions, and would become even less so in coming decades if that mix was not altered in the anticipation of climate change. That is, grape varieties in many (especially the warmest) regions will have to keep changing, or wineries will have to seek fruit from higher latitudes or elevations if they wish to retain their current mix of varieties and wine styles.