Macrowine 2021
IVES 9 IVES Conference Series 9 NMR profiling of grape musts from some italian regions

NMR profiling of grape musts from some italian regions

Abstract

With wine fraud, being a widespread problem [1], the need for more sophisticated and precise analytical methods of its detection remains ever persistent. Nuclear magnetic resonance (NMR) spectroscopy has been widely used for analysis of wine in recent years [2,3], but wine musts were much less studied; in fact, only one paper dealt with the NMR spectra of actual musts [4]. Difficulties arise mostly because grape musts are “live” objects, which undergo rapid fermentation at room temperature, if not inhibited either by freezing or chemical preservative; but even such measures are not sufficient to halt it completely [5]. We have investigated over 300 samples of grape must from 17 of 20 different Italian regions using 1H NMR spectroscopy with water signal suppression, postprocessing in the MatLab software with dynamic alignment [6] and optimized binning [7] to alleviate the effect of fermentation on the chemical shifts of mobile protons. After that, multivariate statistics was performed with techniques such as PCA, PLS-DA and OPLS-DA with respect to various group parameters such as regions, vitivinicultural zones, harvest periods and grape varieties. Advantages and drawbacks of each method were addressed.

DOI:

Publication date: September 13, 2021

Issue: Macrowine 2021

Type: Article

Authors

Pavel Solovyev

Fondazione Edmund Mach (FEM), via E. Mach 1, 38010, San Michele all’Adige, Italy ,Matteo Perini, Fondazione Edmund Mach (FEM), via E. Mach 1, 38010, San Michele all’Adige, Italy Pietro Franceschi, Fondazione Edmund Mach (FEM), via E. Mach 1, 38010, San Michele all’Adige, Italy Luana Bontempo, Fondazione Edmund Mach (FEM), via E. Mach 1, 38010, San Michele all’Adige, Italy Federica Camin, Fondazione Edmund Mach (FEM), via E. Mach 1, 38010, San Michele all’Adige, Italy, University of Trento, via Mach 1, 38010 San Michele all’Adige (TN), Italy

Contact the author

Keywords

grape musts, nmr spectroscopy, profiling, multivariate statistics

Citation

Related articles…

NADES extraction of anthocyanins derivatives from grape pomace

Grape pomace is one of the main by-products generated after pressing in wine-making. It’s valorization through the extraction of bioactive compounds is the answer for the development of sustainable processes. Nevertheless, in the recovery of anthocyanins derivatives, the extraction stage continues to be a limiting step. The nature of the sample and the type of solvent determine the efficiency of the process

Elaboration des cartes conseils pour une gestion du terroir à l’échelle parcellaire: utilisation d’algorithmes bases sur des paramètres physiques du milieu naturel

The “Anjou Terroirs” programme aims at bringing the necessary scientific basis for a ratio­nal and reasoned exploitation of the technical itinerary of the terroir. The scale study is 1/12500. For the mapping, many parameters, such as the granulometry or the depth of soil are observed to each point of caracterisation.

The influence of external factors on the alcoholic fermentation of wine yeasts

Wine yeast strains Saccharomyces ellipsoideus have important applications in food industry and in this regard is sought isolation as pure cultures and selecting those strains

Nitrogen – Lipid Balance in alcoholic fermentations. Example of Champagne musts

Nutrient availability – nitrogen, lipids, vitamins or oxygen – has a major impact on the kinetics of winemaking fermentations. Nitrogen is usually the growth-limiting nutrient and its availability determines the fermentation rate, and therefore the fermentation duration. In some cases, in particular in Champagne, grape musts have high nitrogen concentrations and are sometimes clarified with turbidity below 50 NTU. In these conditions, lipid deficiencies may occur and longer fermentations can be observed. To better understand this situation, a study was realized using a synthetic medium simulating the composition of a Champagne must : 180 g/L of sugar, 360 mg/L of assimilable nitrogen and a lipid content ranging from 1 to 8 mg/L of phytosterols (mainly β-sitosterol).

Berry weight loss in Vitis vinifera (L.) cultivars during ripening

Berry shriveling (BS) in vineyards are caused by numerous factors such as sunburn, dehydration, stem necrosis. Climate change results in an increase in day and night temperatures, rainfall throughout the year, changes in the timing and quantities, long dry summers and a combination of climatic variability such as floods, droughts and heatwaves). Grape development and its composition at harvest is influenced by the latter as grape metabolites are sensitive to the environmental conditions. The grape berry experiences water loss and an increase in flavour development as a result of the BS. An increased sugar content in grapes will result in higher alcohol wines and concentration of grape aromas which may be detrimental to the final wine quality.