Macrowine 2021
IVES 9 IVES Conference Series 9 Ability of Saccharomyces cerevisiae strains to modulate the aroma of albariño wines

Ability of Saccharomyces cerevisiae strains to modulate the aroma of albariño wines

Abstract

The objective of the present work is to evaluate the impact of three S. cerevisiae strains on the comprehensive aroma profile of Albariño wine along its shelf life.

The strains Lalvin QA23TM, Lalvin SauvyTM and Affinity ECA5TM (Lallemand Bio) fermented a model must containing precursors of polyfunctional mercaptans (PFMs) and a polyphenolic and aroma precursor fraction1 extracted from Albariño grapes. Wines were submitted to accelerated anoxic aging at 50 ºC during 1, 2, 5, 8 weeks and at 75 ºC during 12, 24, 48, 72h of aging, respectively. Fermentative aroma compounds, SO2, Strecker aldehydes, and varietal aroma compounds were determined by GC, using six different analytical methods.

The aroma profiles of the Albariño wines obtained are characterized by low amounts of volatile phenols, vanillin derivatives and TDN precursors and by medium to high levels of linalool, β-damascenone, rose oxide, γ-nona and γ-decalactones, which explain the typical and subtle floral aroma notes associated with Albariño wines2. Levels of linalool faded during aging, but floral notes may be partially compensated by increasing levels of ethyl cinnamate.

The ability of the strains assayed to modulate levels of terpenes and lactones was limited citronellol and rose oxide. They were able to influence slightly but significantly levels of β-damascenone and ethyl cinnamate in aged wines. This suggests that the influence of the strains on floral notes is significant, but not dominant3. In clear contrast, the strains introduced a great variability in the levels of PFMs which mostly remained all along wine shelf life.

Even if aging was carried out under strict anoxic conditions, levels of Strecker aldehydes increased, isobutanal and 2-methylbutanal in a strain-dependent way, suggesting that Strecker degradation of amino acids took place with already present wine α-dicarbonyls. Levels of diacetyl and isovaleric acid increased during aging, in spite of the fact that aging conditions were not adequate for microbial development.

Regarding fermentative compounds, levels of higher alcohols and their acetates, straight and branched chain fatty acids and their ethyl esters as well as Strecker aldehydes were strongly strain-dependent. Except for acetates, differences were maintained during aging or even intensified in the cases of aldehydes and ethyl esters of branched acids.

Finally, aging at 50 and 75 ºC were in general very well correlated, suggesting that aging at 75ºC can satisfactorily predict evolution during aging of many wine components. aging at 75ºC can satisfactorily predict evolution during aging of many wine components, except PFMs and Strecker aldehydes.

S. cerevisiae strains can be used to produce Albariño wines with completely different sensory profiles and different sensory evolutions during aging. While the effects on varietal floral and sweet aroma compounds was just moderate, effects on PFMs and fermentative aroma compounds, including Strecker aldehydes were very large.

DOI:

Publication date: September 14, 2021

Issue: Macrowine 2021

Type: Article

Authors

Marie Denat 

Laboratory for Aroma Analysis and Enology (LAAE), University of Zaragoza, Instituto Agroalimentario de Aragón (IA2) (UNIZAR-CITA), Zaragoza (Spain)  ,Vicente FERREIRA, (LAAE), University of Zaragoza, Instituto Agroalimentario de Aragón (IA2), Zaragoza (Spain) Ignacio ONTAÑÓN, (LAAE), University of Zaragoza, Instituto Agroalimentario de Aragon (IA2), Zaragoza (Spain)

Contact the author

Keywords

cerevisiae, fermentation, wine aging, albariño, polyfunctional mercaptans, strecker aldehydes

Citation

Related articles…

Adaptability of grapevines to climate change: characterization of phenology and sugar accumulation of 50 varieties, under hot climate conditions

Climate is the major factor influencing the dynamics of the vegetative cycle and can determine the timing of phenological periods. Knowledge of the phenology of varieties, their chronological duration, and thermal requirements, allows not only for the better management of interventions in the vineyard, but also to predict the varieties’ behaviour in a scenario of climate change, giving the wine producer the possibility of selecting the grape varieties that are best adapted to the climatic conditions of a certain terroir. In 2014, Symington Family Estates, Vinhos, established two grape variety libraries in two different places with distinctive climate conditions (Douro Superior, and Cima Corgo), with the commitment of contributing to a deeper agronomic and oenological understanding of some grape varieties, in hot climate conditions. In these research vineyards are represented local varieties that are important in the regional and national viticulture, but also others that have over time been forgotten — as well as five international reference cultivars. From 2017 to 2021, phenological observations have been made three times a week, following a defined protocol, to determine the average dates of budbreak, flowering and veraison. With the climate data of each location, the thermal requirements of each variety and the chronological duration of each phase have been calculated. During maturation, berry samples have been gathered weekly to study the dynamics of sugar accumulation, between other parameters. The data was analysed applying phenological and sugar accumulation models available in literature. The results obtained show significant differences between the varieties over several parameters, from the chronological duration and thermal requirements to complete the various stages of development, to the differences between the two locations, confirming the influence of the climate on phenology and the stages of maturation, in these specific conditions.

δ13C : A still underused indicator in precision viticulture  

The first demonstration of the interest of carbon isotope composition of sugars in grapevine, as an integrated indicator of vineyard water status, dates back to 2000 (Gaudillère et al., 1999; Van Leeuwen et al., 2001). Thanks to the isotopic discrimination of Carbon that takes place during plant photosynthesis, under hydric stress conditions, it is possible to accurately estimate the photosynthetic activity. Ever since, δ13C has been widely applied with success to zonation, terroir studies and vine physiology research, but is still not widely used by viticulturists. This is quite astonishing by considering the impact of global warming on viticulture and the need to improve water management, that would justify a widespread use of δ13C.
The lack of private laboratories proposing the analysis, the cost of the technology, as well as the long analytical delays, have been detrimental to its development. Some laboratories tried to overcome the analytical difficulties of isotopic analysis by using fourier transformed infrared spectroscopy, as a fast and cheap alternative to the official OIV method (IRMS). These claimed FTIR models have never been published or peer reviewed and cannot be considered robust. In this work, thanks to the recent acquisition of IRMS technology, new modern and robust applications of δ13C for viticulture are proposed. This includes the use of the analysis to make parcel separations at harvesting, the possibility to increase the precision of hydric stress cartography and the potential cost reduction when compared with Scholander pressure bomb analysis.

Extreme canopy management for vineyard adaptation to climate change: is it a good idea?

Climate change constitutes an enormous challenge for humankind and for all human activities, viticulture not being an exception. Long-term strategic changes are probably needed the most, but growers also need to deal with short-term changes: summers that are getting progressively warmer, earlier harvest dates and higher pH in musts and wines. In the last 10-15 years, a relevant corpus of research is being developed worldwide in order to evaluate to which extent extreme canopy management operations, aimed at reducing leaf area and, thus, limiting the source to sink ratio, could be useful to delay ripening. Although extreme canopy management can result in relevant delays in harvest dates, longer term studies, as well as detailed analysis of their implications on carbohydrate reserves, bud fertility and future yield are desirable before these practices can be recommended.

Use of a new, miniaturized, low-cost spectral sensor to estimate and map the vineyard water status from a mobile 

Optimizing the use of water and improving irrigation strategies has become increasingly important in most winegrowing countries due to the consequences of climate change, which are leading to more frequent droughts, heat waves, or alteration of precipitation patterns. Optimized irrigation scheduling can only be based on a reliable knowledge of the vineyard water status.

In this context, this work aims at the development of a novel methodology, using a contactless, miniaturized, low-cost NIR spectral tool to monitor (on-the-go) the vineyard water status variability. On-the-go spectral measurements were acquired in the vineyard using a NIR micro spectrometer, operating in the 900–1900 nm spectral range, from a ground vehicle moving at 3 km/h. Spectral measurements were collected on the northeast side of the canopy across four different dates (July 8th, 14th, 21st and August 12th) during 2021 season in a commercial vineyard (3 ha). Grapevines of Vitis vinifera L. Graciano planted on a VSP trellis were monitored at solar noon using stem water potential (Ψs) as reference indicators of plant water status. In total, 108 measurements of Ψs were taken (27 vines per date).

Calibration and prediction models were performed using Partial Least Squares (PLS) regression. The best prediction models for grapevine water status yielded a determination coefficient of cross-validation (r2cv) of 0.67 and a root mean square error of cross-validation (RMSEcv) of 0.131 MPa. This predictive model was employed to map the spatial variability of the vineyard water status and provided useful, practical information towards the implementation of appropriate irrigation strategies. The outcomes presented in this work show the great potential of this low-cost methodology to assess the vineyard stem water potential and its spatial variability in a commercial vineyard.

austrianvineyards.com: online viewer of all designations of Austrian wine

To digitally record and present all the origins of Austrian wines in the same perfect and clear way was the motivation for the Austrian Wine Marketing Board (Austrian Wine) to start with the project in 2018. In June 2021 the results were presented to the public in an online viewer showing all the designations of Austrian wine, available at https://austrianvineyards.com in a largely barrier-free manner. The online viewer provides tailored individual maps fitted to the respective zoom level. The smallest unit of wine-origins in Austria is called Ried and is displayed in a plot-specific manner highlighting areas under vine. Information on the Ried include administrative district, winegrowing municipality, cadastral municipality, large collective vineyard site, specific winegrowing region, generic winegrowing region, winegrowing area and, in many cases, an illustrative picture. Complementary data on the size, elevation (minimum-maximum), orientation (in 8 sectors plus flat) and gradient (minimum, maximum, average) are based on the area under vine according to the EU’s Integrated Administration and Control System. Additional information covers climate data. The diagrams are taken from the monthly breakdown of data in the annals of the Central Institute for Meteorology and Geodynamics, Austria provide a display of values for air temperature, precipitation, and sunshine hours for the reference year and the long-term average. Seasonal aggregated data on temperature, precipitation, and sunshine hours complete the display. Short descriptions with emphasis on geology and soil, field name in historical maps, etymology of the denomination, and main planted variety complements the available information for the main designations in the online viewer. These descriptions are compiled by winegrowers, geologists, historians, and journalists. All the information and data can be extracted to a pdf-file. Printed vineyard maps are also available. Missing content regarding wine origins in Styria will be completed in winter 2021/22.