Soils, climate and vine management: their influence on Marlborough Sauvignon blanc wine style

Micro(mi)RNAs are small non-coding RNAs that regulate several pathways and are widely recognised as key players in plant development, tissue differentiation, and many other important physiological processes, including plant adaptation to biotic and abiotic stresses. The release of plant genomes and the application of high throughput sequencing have considerably extended miRNA discovery across many species, including grapevine (Vitis spp.). Despite their relevance in plant development, functional studies in grapevine to clarify the function of miRNAs are not yet available. Through the grapevine genetic improvement platform IMPROVIT at CNR-IPSP (http://www.ipsp.cnr.it/en/thematics/turin-headquarter-thematics/improvit/), we developed integrated approaches to discover miRNA function in grapevine.

Grapevines are susceptible to freezing damage at temperatures below -5°F during the winter season. Preventing winter injury to grapevines is a major challenge in many grape-producing regions. Conventional methods such as hilling-up soil over graft unions have been developed as winter protection methods for preventing vine loss. However, these practices have drawbacks such as soil erosion, vine damage and crown gall development.

L’histoire de viticulture de l’Afrique du Sud embrasse 340 ans, et a commencé, à la province du Cap, où les colonisateurs hollandais ont planté les premières vignes. L’arrivée des Huguenots français en 1688 a avancé, le développement.

This paper describes a zoning study performed on a vast territory of around 86,500 hectares, situated in the countryside area of La Mancha Central (Castilla-La Mancha). The aim of the study was to classify the environment according to a small number of ecological criteria, establish the relevant territorial units and generate thematic maps with the different levels of criteria employed and synthetic maps by crossing these criteria.

Developments in high-throughput sequencing (HTS) technologies allow us to obtain large amounts of microbial information from wine and must samples. Thus approaches, that are aimed at characterising the microbial diversity during fermentation, can be enhanced, or possibly even replaced, with HTS-based metabarcoding. To reduce experimental biases and increase data reproducibility, we compared 3 DNA extraction methods by evaluating differences in the fungal diversity with Riesling alcoholic fermentation samples at four different vineyards. The fungal diversity profiling was done using the genetic markers ITS2 and D2 using metabarcoding. The extraction methods compared consisted of a commercial kit, a recently published protocol that includes a DNA enhancer, and a protocol based on a buffer containing common inhibitor removal reagents. All methods were able to distinguish vineyard effects on the fungal diversity, but the results differed quantitatively.