Comparative studies on the dynamics of fermentation of selected wine yeasts

Aim: Through machine-learning modelling of plant water status from environmental characteristics, this work aims to develop a model able to predict grape phenolic composition in space and time to guide selective harvest decisions at the estate scale.

After Champagne popping, the first consumer’s observation is the shape of the cork stopper. Consumers expect a “mushroom shape”. Nevertheless, we sometimes observe a “barrel” shape due to inappropriate cork’s elastic properties. The aim of this study was to follow the loss of cork stopper resiliency during 26 months according to the density (d) of the cork in contact with the wine. 1680 disks were weighed + measured and divided in 6 density classes: High (H1 d= 0,19 g/cm3 – H2 d= 0,21 g/cm3), Medium (M, not studied) and Low (L1 d= 0,13 g/cm3 – L2 d= 0,14 g/cm3). Then, 138 technical cork stoppers were produced for each of the 4 studied groups. These corks consisted of an agglomerated natural cork granule body to which two natural cork disks were glued. A total of 552 bottles of sparkling wine were closed with these corks and open after 13, 19 and 26 months to follow cork resiliencies. Wine bottles were stored horizontally; thus, the external natural cork disks were in contact to the wine. During the 26 months of the study, highly significant differences (ANOVA) were observed between the resiliencies of H-corks and those of L-corks, whatever the time studied. The diameters of the L-corks were statistically higher than those of the H-corks. No significant differences were observed between L1 and L2 corks. At the opposite, differences were noted between H1 and H2 at 19 and 26 months. This could be explained by the heterogeneity of the resiliency that was higher for H-corks than for L-corks. Finally, the corks were visually (12 judges) divided in 3 classes corresponding to high (expected mushroom shape, i.e high resiliency), medium (irregular shape of the disk in contact with the wine and/or low premature deterioration of the expected resiliency) and low qualities (barrel shape = premature deterioration of the resiliency). The corks were also divided in 3 categories corresponding to 0-33%, 34-66% and 67-100% resiliency. A strong correlation was noted between the visual and the instrumental categorizations. This study strongly evidenced 1) the importance of the cork density on the cork stopper behaviour when opening the bottle and 2) the interest of an instrumental approach reflecting the consumer’s perception.

Wine is a solution containing abundant volatile compounds which contribute to their aroma. Many of them are produced by yeast as metabolism by-products. Different yeast strains produce different volatile profiles. The possibility of studying the evolution of volatile compounds during fermentation, using sampling methods that not alter the volume of fermentation media, is of great interest. In spite of this, non-invasive methods to monitoring the evolution of volatile profile during fermentation have been seldom used. The goals of this work were to use by first time the headspace sorptive extraction (HSSE) as non-invasive method to monitor the evolution of volatile profiles throughout alcoholic fermentation and to study the changes on volatile profiles produced by Saccharomyces cerevisiae and Lachancea thermotolerans during fermentation of a must with high sugar content.

Multispectral unmanned aerial vehicle (UAV) has been successfully applied in monitoring vine vegetative growth, however, there are still potential relationships between remote sensing vegetation indexes (vis) and vine condition or grape quality, so it is worthy a deeper investigation to make a better use of UAV. One of the purposes of the study is to find out vis that could denote the severity of downy mildew (DM), so that precise and differentiated control strategies would be adopted subsequently.

Regulated deficit irrigation (RDI) is a common viticultural practice for wine grape production. In addition to the potential improvement of water use efficiency, the adoption of this technique favors smaller canopies with higher levels of fruit sun exposure, enhancing quality attributes associated with red wine grapes such as smaller berries with higher tannins and anthocyanins. However, these quality attributes do not necessarily transfer to white wine grapes. The goal of this project was to assess whether partial rootzone drying (PRD) is more suited than RDI to grow high-end white wine grapes in arid climates, especially aromatic varieties, using Riesling as a model.