GiESCO 2019 banner
IVES 9 IVES Conference Series 9 GiESCO 9 GiESCO 2019 9 Exploring the plasticity of the grapevine drought physiology

Exploring the plasticity of the grapevine drought physiology

Abstract

Context and purpose of the study ‐ Grapevine response to water deficit has been extensively studied. Nevertheless, debate still exists regarding some physiology adoption under drought, e.g. vulnerability to cavitation or iso‐anisohydric classification, among others. Discrepancies between published results, other than specific experimental setup, are attributed to environment/climate conditions and genotypes used. Indeed, the same genotype could exhibit a different phenotype under different climates (i.e. phenotypic plasticity). To date little information is available regarding the plasticity extent of certain traits related to drought response in grapevines. Here we present the results of a novel experiment, where a single genotype was exposed to similar water stress conditions in two different locations characterized by different climatic conditions.

Material and methods ‐ 90 plants of Grüner Veltliner grafted on 5BB rootstock were grown in pots during the 2016 season in Tulln (N‐E Austria). In January 2017 and after pruning, half of the vines were transported to Udine (N‐E Italy). In both locations, vines were re‐potted in 20L pots and filled with the same commercial potting media supplemented with 30% perlite. Then, pots were arranged in rows that were covered using a plastic film roof to prevent rain. Water was supplemented by drip irrigation and a set of vines were positioned under weighting mini‐lysimeters to measure ETc. Climate data was recorded by a weather station in each site. Vines were irrigated daily to 100% ETc until the imposition of water stress (WS) towards the end of June (pre‐veraison, E‐L 33). WS vines were daily irrigated 30% ETc of WW, ecophysiological data recorded frequently, and berry/leaf tissues sampled. The experiment was carried out for two consecutive vintages on the same vines.

Results ‐ Climatic variables were different between sites and deficit irrigation based on ETc yielded different results in terms of water stress intensity. In both years, well‐watered controls in Tulln never reached water potential below ‐0.7 MPa while those in Udine reached values as low as ‐1.2 MPa. Although the pot volumes and soil type used was the same, differences in atmospheric water demand determined different irrigation volumes between sites. Also, WS vines in Udine reached Ψstem values much lower than in Tulln, impacting differently as well some berry ripening parameters. Interestingly, the stomatal conductance (gs) response to Ψstem was different between sites: in Udine gs reached values <50 mmol H2O m‐2 s‐2 at Ψstem values much lower (‐1.2 MPa) than in Tulln (‐0.8 MPa), showing how gs/Ψ often used as indicators for iso‐anisohydric classification are influenced by environmental conditions. Finally, the WUEi was different among sites in 2018, determining a better performance of the vines in Tulln compared with those in Udine. 

DOI:

Publication date: June 19, 2020

Issue: GiESCO 2019

Type: Article

Authors

Jose Carlos HERRERA (1), Katja ŠUKLJE (2), Stefania SAVOI (1), Alberto CALDERAN (3), Lorena BUTINAR (2), Enrico PETERLUNGER (3), Giorgio ALBERTI (3), Paolo SIVILOTTI (3), Astrid FORNECK (1)

(1) Universität für Bondenkultur (BOKU), Institute of Viticulture and Pomology, Konrad‐Lorenz Str. 24, 3430 Tulln, Austria
(2) University of Nova Gorica, Wine Research Centre, Glavni trg 8, 5271 Vipava, Slovenia
(3) University of Udine, Department of Agricultural, Food, Environmental and Animal Sciences, via delle Scienze 206, 33100 Udine, Italy

Contact the author

Keywords

deficit irrigation, water relations, berry ripening

Tags

GiESCO 2019 | IVES Conference Series

Citation

Related articles…

Impact of climate change on the viticultural climate of the Protected Designation of Origin “Jumilla” (SE Spain)

Protected Designation of Origin “Jumilla” (PDO Jumilla) is located in the Spanish provinces of Albacete and Murcia, in the South-eastern part of the Iberian Peninsula, where most of the models predict a severe impact of climate change in next decades. PDO Jumilla covers an area of 247,054 hectares, of which more than 22,000 hectares

Assessing the relationship between cordon strangulation, dieback, and fungal trunk disease symptom expression

Grapevine trunk diseases including Eutypa dieback are a major factor in the decline of vineyards and may lead to loss of productivity, reduced income, and premature reworking or replanting. Several studies have yielded results indicating that vines may be more likely to express symptoms of vascular disease if their health is already compromised by stress. In Australia and many other wine-growing regions it is a common practice for canes to be wrapped tightly around the cordon wire during the establishment of permanent cordon arms. It is likely that this practice may have a negative effect on health and longevity, as older cordons that have been trained in this manner often display signs of decay and dieback, with the wire often visibly embedded within the wood of the cordon. It is possible that adopting a training method which avoids constriction of the vasculature of the cordon may help to limit the onset of vascular disease symptom expression. A survey was conducted during the spring of two consecutive growing seasons on vineyards in South Australia displaying symptoms of Eutypa lata infection when symptomless shoots were 50–100 cm long. Vines were assessed as follows: (i) the proportion of cordon exhibiting dieback was rated using a 0–100% scale; (ii) the proportion of canopy exhibiting foliar symptoms of Eutypa dieback was rated using a 0–100% scale; (iii) the severity of strangulation was rated using a 0–4 point scale. Images were also taken of each vine for the purpose of measuring plant area index (PAI) using the VitiCanopy App. The goal of the survey was to determine if and to what extent any correlation exists between severity of strangulation and cordon dieback, in addition to Eutypa dieback foliar symptom expression.

Variations of soil attributes in vineyards influence their reflectance spectra

Knowledge on the reflectance spectrum of soil is potentially useful since it carries information on soil chemical composition that can be used to the planning of agricultural practices. If compared with analytical methods such as conventional chemical analysis, reflectance measurement provides non-destructive, economic, near real-time data. This paper reports results from reflectance measurements performed by spectroradiometry on soils from two vineyards in south Brazil. The vineyards are close to each other, are on different geological formations, but were subjected to the same management. The objective was to detect spectral differences between the two areas, correlating these differences to variations in their chemical composition, to assess the technique’s potential to predict soil attributes from reflectance data.To that end, soil samples were collected from ten selected vine parcels. Chemical analysis yield data on concentration of twenty-one soil attributes, and spectroradiometry was performed on samples. Chemical differences significant to a 95% confidence level between the two studied areas were found for six soil attributes, and the average reflectance spectra were separated by this same level along most of the observed spectral domain. Correlations between soil reflectance and concentrations of soil attributes were looked for, and for ten soil traits it was possible to define wavelength domains were reflectance and concentrations are correlated to confidence levels from 95% to 99%. Partial Least Squares Regression (PLSR) analyses were performed comparing measured and predicted concentrations, and for fifteen out of 21 soil traits we found Pearson correlation coefficients r > 0.8. These preliminary results, which have to be validated, suggest that variations of concentration in the investigated soil attributes induce differences in reflectance that can be detected by spectroradiometry. Applications of these observations include the assessment of the chemical content of soils by spectroradiometry as a fast, low-cost alternative to chemical analytical methods.

Characterization of variety-specific changes in bulk stomatal conductance in response to changes in atmospheric demand and drought stress

In wine growing regions around the world, climate change has the potential to affect vine transpiration and overall vineyard water use due to related changes in atmospheric demand and soil water deficits. Grapevines control their transpiration in response to a changing environment by regulating conductance of water through the soil-plant-atmosphere continuum. Most vineyard water use models currently estimate vine transpiration by applying generic crop coefficients to estimates of reference evapotranspiration, but this does not account for changes in vine conductance associated with water stress, nor differences thought to exist between varieties. The response of bulk stomatal conductance to daily weather variability and seasonal drought stress was studied on Cabernet-Sauvignon, Merlot, Tempranillo, Ugni blanc, and Semillon vines in a non-irrigated vineyard in Bordeaux France. Whole vine sap flow, temperature and humidity in the vine canopy, and net radiation absorbed by the vine canopy were measured on 15-minute intervals from early July through mid-September 2020, together with periodic measurement of leaf area, canopy porosity, and predawn leaf water potential. From this data, bulk stomatal conductance was calculated on 15-minute intervals, and multiple regression analysis was performed to identify key variables and their relative effect on conductance. Attention was focused on addressing multicollinearity and time-dependency in the explanatory variables and developing regression models that were readily interpretable. Variability of vapor pressure deficit over the day, and predawn water potential over the season explained much of the variability in conductance, with relative differences in response coefficients observed across the five varieties. By characterizing this conductance response, the dynamics of vine transpiration can be better parameterized in vineyard water use modeling of current and future climate scenarios.

How distinctive are single vineyard Gewürztraminer musts and wines from Alto Adige (Italy) based on untargeted analysis, sensory profiling, and chemometric elaboration?

Vitis vinifera L. ‘Gewürztraminer’ is a historical grape variety of Alto Adige (Südtirol), Italy, which is widely grown in the area of Tramin an der Weinstraße, but is also grown globally. It produces highly aromatic wines that are strongly influenced by the terroir of the vineyard sites where they are grown. This study looked at musts and young wines from ‘Gewürztraminer’ grapes harvested in seven distinct vineyards near Tramin and then processed at Cantina di Termeno, minimizing winemaking protocol variability. Samples were profiled using bidimensional gas chromatography–time-of-flight mass spectrometry, liquid chromatography coupled to electrochemical detection, and near-IR spectrometry. The data were subjected to Principle Component Analysis and Hierarchical Clustering Analysis. Sensory discriminant testing was undertaken using the sorting method with a semi-trained panel, and the data were processed using Multidimensional Scaling. Seven must/wine pairs could be distinguished based on their untargeted volatilome profiles and on sensory evaluation. As expected, there were greater differences in the volatile compounds between the wines than between the musts. The wines from vineyards 4 and 5 were nonetheless quite homogenous in terms of chemical and sensory analyses, as were the wines from vineyards 1 and 3. For the phenolic profile, differences were noted between the musts and wines of vineyards 2, 3, and 4, but the musts from vineyards 5 and 7 were similar. Sensory analysis showed the wines from vineyards 6 and 7 to be distinct from the rest. These results reinforce that the composition of ‘Gewürztraminer’ musts and wines is strongly determined by vineyard site, even in a small geographic area with high variability of the terroir (soil and microclimate), and that these differences are apparent in the flavours and aromas of the finished wines. Further confirmation would require a larger sample of wines, preferably from several vintages.