Macrowine 2021
IVES 9 IVES Conference Series 9 Fining-Derived Allergens in Wine: from Detection to Quantification

Fining-Derived Allergens in Wine: from Detection to Quantification

Abstract

Since 2012, EU Commission approved compulsory labeling of wines treated with allergenic additives or processing aids “if their presence can be detected in the final product” (EU Commission Implementing Regulation No. 579/2012 of 29 June 2012). The list of potential allergens to be indicated on wine labels comprises sulphur dioxide and milk- and egg- derived fining agents, including hen egg lysozyme, which is usually added in wines as preservative. In some non-EU countries, the list includes gluten, tree nuts and fish gelatins. With the exception of lysozyme, all these fining proteins were long thought to be totally removed by subsequent winemaking processings (e.g. bentonite addition). Suitable methods to detect and quantify potential residual allergens are necessary to ensure compliance with legislation. ELISA kits are routinely used to ensure the absence of allergenic proteins in wines, since they are easy to perform. The required LOD and LOQ for milk and egg products with ELISA methods are 0.25 ppm and 0.5 ppm (OIV Comex 502- 2012). In a recent paper (1), no detectable egg white protein residue was found in red wines by ELISA, even when coadiuvant was added at 660 ppm, without following bentonite treatment. In 2011, a clinical survey (2) reported that, although no allergen residue was detected by ELISA kits in the fined wines, positive skin prick test reactions and basophil activation to the treated wines were observed in the majority of patients with allergy to milk, egg or fish, correlating with the concentration of the fining agents used. From these findings, some uncentainty about the safety of wines certified as “allergen-free” through ELISA methods can exist. More recently, mass spectrometry have been proposed, mainly as a confirmatory method of results from ELISA. The two main drawbacks for MS-based methods, especially in the past, were insufficient LOD and LOQ, and high costs. At present, some methods with competitive performance and linearity have been set up for milk and egg protein based fining aids. Aim of the present presentation is to review the different approaches in the detection of allergenic residues in wines, including recent metrological approaches, and development of innovative biosensors. Preliminary data on MS-based method that is currently being developed in our laboratory will be presented, as well future perspectives on the topic and possible implications for consumers health, safety and ethical preferences.

1- Uberti F., et al. Immunochemical investigation of allergenic residues in experimental and commercially-available wines fined with egg white proteins. Food Chem. 2014;159:343-52. 2- Vassilopoulou E., et al. Risk of allergic reactions to wine, in milk, egg and fish-allergic patients. Clin Transl Allergy. 2011;1:10.

Publication date: May 17, 2024

Issue: Macrowine 2016

Type: Poster

Authors

Maria Gabriella Giuffrida*, Cristina Lamberti, Daniela Gastaldi, Laura Cavallarin, Marzia Giribaldi

*ISPA

Contact the author

Tags

IVES Conference Series | Macrowine | Macrowine 2016

Citation

Related articles…

A combination of biotechnology tools and coopers elements for an alternative the addition of SO2 at the end of the malolactic fermentation in red wines or at the “mutage” for the “liquoreux” wines

In red wines the post-MLF SO2 addition is an essential event. It is also the case for the “mutage” during the elaboration of the “liquoreux”. At these moments SO2 plays an antimicrobial action and an antioxidant effect. But at current pH of wines, ensuring a powerful molecular SO2 has become very difficult. Recent work on Brettanomyces strains have also shown that some strains are resistant up to 1.2 mg / L of molecular SO2. It’s also the case of the some Saccharomuces or Zygosaccharomyces strains suitable to re-ferment “liquoreux” wines after the “mutage”.

Comprehensive exploration of wine aroma-related compounds as promoted by alternative vinification procedures in case of Zelen (Vitis vinifera L.) grapes processing

Not only vintner’s decisions in the vineyard, but also winemaker’s choices of technology approaches in the cellar play a significant role in the final wine style and quality. Whereas traditional technologies within chosen terroir are quite well explored and thus somehow predictable, there is no proper knowledge available on possible outcomes in case of implementing novel, alternative winemaking strategies. To reveal their effects on wine aroma compounds and sensory characteristics, two alternative strategies
(cryoextraction or addition of whole grape berries during last stages of fermentation) were compared to classical Vipava valley winemaking approach as normally used for an autochthonous variety Zelen. After separate vinification and bottling, all the experimental wines were subjected to semiquantitative metabolic profiling of volatile compounds (VOCs) by means of GC/MS and were then also sensorialy evaluated by pre-trained panel.

Foam characteristics of white, rosé and red sparkling wines elaborated by the champenoise method

Contribution Foam is the characteristic that differentiates sparkling wines from still wines, being the first sensory attribute that tasters and consumers perceive and that determines the final quality of sparkling wines [1]. The foaming properties mainly depend on the chemical composition of wines [2-3], and different factors involved in wine composition will have an effect on foam quality. In Spain, the sparkling wine market focuses on the production of white and rosé sparkling wine, with very low production of red sparkling wines. However, this type of wines is elaborated in countries like Australia, South-Africa, Argentina, Italy or Portugal, with a great acceptance by consumers. No studies on the foaming characteristics of red sparkling wines have been found.

Impact of some agronomic practices on grape skins anthocyanin content

Wine colour is the first quality characteristic to be assessed, especially regarding red wines. Anthocyanins are very well known to be the main responsible compounds for red wine colour. Red cultivars can synthesize and accumulate anthocyanins in berry skin to express their colour. However, anthocyanin accumulation is often influenced by a series of factors, such as genetic regulation, phytohormones, environmental conditions and viticultural management.

Characterization of free and glycosidically bound simple phenols in hybrid grape varieties using liquid chromatography coupled to high resolution mass (q-orbitrap)

Vitis vinifera is one of the most diffused grapevines over the word and it is the raw material for high quality wines production. The availability of more resistant interspecific hybrid vine varieties, developed from crosses between Vitis vinifera and other Vitis species, has generating much interest, also due to the low environmental effect of production. However, hybrid grape wine composition and varietal differences between interspecific hybrids are not well defined. Different studies revealed that wine consumption has health effects due to its high content of antioxidants, as phenolic compounds. In particular, simple phenols are appreciated not only for their physiological health benefits, including antioxidant, anti-inflammatory and cardioprotective effects, but also because they affect wines organoleptic profile and have a significant role in defining their nutritional characteristics.