terclim by ICS banner
IVES 9 IVES Conference Series 9 Selection of beneficial endophytes from Sicilian grapevine germplasm 

Selection of beneficial endophytes from Sicilian grapevine germplasm 

Abstract

The recent expansion of arid areas due to climate change is putting grapevine and the other traditional productions at risk in all Mediterranean countries with a limited availability of fundamental resources such as water. It is possible to improve the resilience of vineyards by developing sustainable agricultural practices based on biological and natural resources such as endophytic microorganisms that colonize inner plant tissues, and which can potentially increase the tolerance to abiotic stresses. A selection of grapevine endophytes was conducted from 2021 to 2023 as part of the PRIMA project PROSIT. In particular, the research aimed to select consortia of endophytic bacteria and/or fungi able to improve the grapevine tolerance to drought. To this aim, vine leaves were harvested during two vegetative seasons, from local varieties subjected to a long-lasting adaptation to arid conditions without regular irrigation. A wide diversity of genera and species belonging to different microbial phyla was isolated on artificial media (NA and PDA) from surface-sterilized grapevine leaves. Selected fungal and bacterial isolates were identified by molecular barcoding based on ITS and 16S rDNA sequences, respectively. Overall, bacterial endophytes were isolated in higher numbers than fungal ones. In 2022, a higher abundance of fungal colonies was isolated at the end of the growing season, suggesting a seasonal dynamic of the microbiota composition. Some of the selected isolates belong to species already known as PGP endophytes of crops, including grapevine. Preliminary in vitro experiments confirmed that those strains can increase the concentration of auxins, ammonium and soluble phosphate.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Dalila Crucitti1*, Irene Doro2, Michela Zottini2, Alessandra Tondello3, Andrea Squartini3, Roberto De Michele1, Francesco Carimi1, Davide Pacifico1

1 IBBR CNR – Institute of Biosciences and Bioresources, via Ugo La Malfa 153, 90146 Palermo
2 Department of Biology, Università degli Studi di Padova, via U. Bassi 58b, 35131 Padova, Italy
3 Department of Agronomy, Animals, Food, Natural Resources, and Environment, DAFNAE Università degli Studi di Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy

Contact the author*

Keywords

drought stress, culturable endophytes, bacteria, fungi

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Cumulative effect of deficit irrigation and salinity on vine responses

Climate change is increasing water needs in most of the wine growing regions while reducing the availability and quality of water resources for irrigation. In this context, the sustainability of Mediterranean viticulture depends on grapevine responses to the combinations of water and salt stress. With this aim, this work studies the effects of deficit irrigation and salinity on the physiology of the Tempranillo cultivar (Vitis vinifera L.) grafted onto a drought and salinity tolerant rootstock (1103 Paulsen).

Influenza dell’esposizione del vigneto sulla maturazione dell’uva

Lo studio è stato condotto in vigneti commerciali di Vitis vinifera cv Nebbiolo localizzati in Piemonte, Italia del Nord-Ovest, intorno alla sommità di una collina. L’obiettivo dello studio è stato di determinare come l’esposizione del vigneto possa influenzare il comportamento vegetativo della vite, il manifestarsi delle fasi fenologiche, e la cinetica di maturazione dell’uva con particolare riguardo all’accumulo di antociani e flavonoli.

Guyot or pergola for dehydration of Rondinella grape

Pergola veronese is the most important vine training system in Valpolicella area but Guyot in the last decades is diffusing. Rondinella is one of the three most important varieties

AGEING BOTTLED WINES SUBMERGED IN SEA: DOES IT IMPACT WINE COMPOSITION?

Aging wines is a common practice in oenology, which in recent years has undergone some innovations. Currently, we are witnessing the practice of aging bottled wine in depth, immersed in the sea or in reservoirs, for variable periods of time, but so far, little is known about the impact of aging in depth on the physicochemical properties, of wines.
The objective of this work was to evaluate the impact of this practice on the physicochemical characteristics, in particular to verify changes in the volatile composition of wines bottled and subsequently immersed in depth. A red wine from Cabernet Sauvignon was bottled and a set of bottles were submerged from July to February (2020), another set of bottles were submerged from February to September (2020) and another set was kept in the wine cellar. Bottles from each set were analyzed (in triplicate) in July 2021.

Grape phylloxera leaf-feeding populations in commercial vineyards – a new biotype ?

Grape Phylloxera (Daktulosphaira vitifoliae Fitch) ordinarily has great difficulty establishing leaf galls on the European Grapevine (VitisviniferaL.). Yet populations of leaf-feeding Phylloxera are increasingly being observed throughout commercial vineyards world-wide. Effective plant protection strategies including quarantine actions are currently missing to fight, grape phylloxera populations in affected vineyards and combat linked negative effects on vines and yield. Contrary to the otherwise mandatory continuous infestation pressure from externally established populations (e.g. from populations developed on rootstock foliage or other interspecific hybrids, these leaf-feeding populations seem to establish themselves annually.