terclim by ICS banner
IVES 9 IVES Conference Series 9 Selection of beneficial endophytes from Sicilian grapevine germplasm 

Selection of beneficial endophytes from Sicilian grapevine germplasm 

Abstract

The recent expansion of arid areas due to climate change is putting grapevine and the other traditional productions at risk in all Mediterranean countries with a limited availability of fundamental resources such as water. It is possible to improve the resilience of vineyards by developing sustainable agricultural practices based on biological and natural resources such as endophytic microorganisms that colonize inner plant tissues, and which can potentially increase the tolerance to abiotic stresses. A selection of grapevine endophytes was conducted from 2021 to 2023 as part of the PRIMA project PROSIT. In particular, the research aimed to select consortia of endophytic bacteria and/or fungi able to improve the grapevine tolerance to drought. To this aim, vine leaves were harvested during two vegetative seasons, from local varieties subjected to a long-lasting adaptation to arid conditions without regular irrigation. A wide diversity of genera and species belonging to different microbial phyla was isolated on artificial media (NA and PDA) from surface-sterilized grapevine leaves. Selected fungal and bacterial isolates were identified by molecular barcoding based on ITS and 16S rDNA sequences, respectively. Overall, bacterial endophytes were isolated in higher numbers than fungal ones. In 2022, a higher abundance of fungal colonies was isolated at the end of the growing season, suggesting a seasonal dynamic of the microbiota composition. Some of the selected isolates belong to species already known as PGP endophytes of crops, including grapevine. Preliminary in vitro experiments confirmed that those strains can increase the concentration of auxins, ammonium and soluble phosphate.

DOI:

Publication date: June 13, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Dalila Crucitti1*, Irene Doro2, Michela Zottini2, Alessandra Tondello3, Andrea Squartini3, Roberto De Michele1, Francesco Carimi1, Davide Pacifico1

1 IBBR CNR – Institute of Biosciences and Bioresources, via Ugo La Malfa 153, 90146 Palermo
2 Department of Biology, Università degli Studi di Padova, via U. Bassi 58b, 35131 Padova, Italy
3 Department of Agronomy, Animals, Food, Natural Resources, and Environment, DAFNAE Università degli Studi di Padova, Viale dell’Università 16, 35020 Legnaro (PD), Italy

Contact the author*

Keywords

drought stress, culturable endophytes, bacteria, fungi

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

INVESTIGATION OF MALIC ACID METABOLIC PATHWAYS DURING ALCOHOLIC FERMENTATION USING GC-MS, LC-MS, AND NMR DERIVED 13C-LABELED DATA

Malic acid has a strong impact on wine pH and the contribution of fermenting yeasts to modulate its concentration has been intensively investigated in the past. Recent advances in yeast genetics have shed light on the unexpected property of some strains to produce large amounts of malic acid (“acidic strains”) while most of the wine starters consume it during the alcoholic fermentation. Being a key metabolite of the central carbohydrate metabolism, malic acid participates to TCA and glyoxylate cycles as well as neoglucogenesis. Although present at important concentrations in grape juice, the metabolic fate of malic acid has been poorly investigated.

Viticultural practices: past, present and future

Practices in viticulture have greatly evolved in the last five decades. There were three objectives: improvement in the quality of the products, reduction in the production costs through mechanization

Influence of methyl jasmonate foliar application to vineyard on grape volatile composition over three consecutive vintages

An alternative to improve grape quality is the application to the vineyard of elicitors. Although these compounds were first used to increase resistance of plants against pathogens, it has been found that they are also able to induce mechanisms involved in the synthesis of phenolic compounds and some amino acids. However, researches about the influence of elicitors on grape volatile composition are scarcely. Therefore, the aim of this work was to study the influence of methyl jasmonate (MeJ) foliar application on grape aroma composition over three consecutive vintages. MeJ was applied to Tempranillo grapevines at a concentration of 10 mM in 2013, 2014, and 2015 years. Control plants were sprayed with water.

Decline of rootstock-mediated physiological responses in Tempranillo grapevines by prolonged extreme conditions

Agriculture faces many global warming challenges especially in the Mediterranean region. Many strategies have been proposed in viticulture to manage global warming. Rootstocks are among them since they may affect water uptake and the scion’s performance.
The study conducted in La Rioja, Spain, aimed to investigate the impact of different rootstocks (1103P and 161-49C) on the performance of the Tempranillo grapevine scion over a three-day cycles under drought and extreme conditions, specifically during a heatwave in July 2022, with maximum air temperatures up to 40ºC and average daily temperatures of 29.1ºC.

Tolerance to sunburn: a variable to consider in the context of climate change

Climate change effects on grapevine phenology and grape primary and secondary metabolites are well described in recent literature. Increasing frequency and intensity of heat waves may be responsible for important yield losses in the future. However, the impact of this event is not so well described in literature. The present study highlights the importance of grape variety tolerance as a mitigation tool to climate change.