Tracking the origin of Tempranillo Tinto through whole genome resequencing and high-throughput genotyping  

The composition of grape berry cell walls was studied on two grape varieties, two years and two maturation levels at the same time as the extraction of anthocyanins and tannins. The chemical composition of skins, seeds, and pulps, focused on polyphenols and polysaccharides, was compared to the chemical composition in polyphenols after extraction from the skins in model solutions or after wine making of the berries. Polyphenols were mainly characterized by UPLC-MS and HPLC-SEC. Polysaccharides were characterized by analysis of the neutral sugar compositions, and also by the CoMPP (comprehensive micropolymer profiling) analysis, a new method which targets the functional groups of cell wall polysaccharides.

Aim: The aim of this work was to evaluate the use of Variable Rate Application technologies based on prescription maps in commercial vineyards with large intra-parcel variability to achieve a more sustainable distribution of Plant Protection Products (PPP)

The research is part of the “Ecovinegoals” project, financed by Interreg Adrion funds. It aims to encourage the adoption and dissemination of agroecological practices in intensive wine-growing areas. The study focuses on cost analysis of the wine-growing landscape enhancement in an organic winery in order to provide a useful tool for winemakers to direct their investments in green infrastructures. One of the Italian pilot areas of the Ecovinegoals project is the Venezia Biodistrict, characterized by viticulture in a flat reclamation area of 105,800 hectares.

Botrytis cinerea (Bc) is one of the main pathogens affecting the cultivated grapevine. A key role in grapevine tissue colonization is played by cell wall (CW) remodeling driven by CW Modifying Enzymes (CWMEs), expressed both by the host and the pathogen. Their action can impact CW integrity and trigger specific immune signaling, thus influencing Bc infection outcome. To further characterize the role of the CW in the grapevine response to Bc, two contrasting genotypes in their resistance to the fungus were artificially inoculated at full bloom. RNA-seq analysis and biochemical characterization of the CW and its modification in samples collected at 24 hours post-inoculation highlighted significant differences between genotypes.

Apart from pro(antho)cyanidins and tannins, other phenolic compounds in wine or grapes have been shown to interact with salivary proteins and may contribute to overall sensory in-mouth sensations [1, 2]. Anthocyanins are the dominant phenolics in red wine and grape skin [3] , so it is expected that they come into contact and interact with salivary proteins after ingestion.