Effect of ozone application for low-input postharvest dehydration of wine grapes 

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Developing a tractable genetic engineering and gene editing system is an essential tool for grapevine. We initiated a plant transformation and biotechnology program at Oregon State University using the grape microvine system (V. vinifera) in 2018 to interrogate gene-to-trait relationships using traditional genetic engineering and gene editing. The microvine model is also used for nanomaterial-assisted RNP, DNA, and RNA delivery. Most reference genomes and annotations for grapevine are collapsed assemblies of homologous chromosomes and do not represent the specific microvine cultivar ‘043023V004’ under study at our institution.

From the effect of global climate change, wildfire occurrence during grape ripening has increased. These wildfires produce smoke that can carry organic compounds to a vineyard. These smoke compounds are adsorbed in the grape berry and result in wines with elevated levels of smoke-related phenols. These wines are described as having a smokey, burnt, and dirty aroma (Kristic et al, 2015). Not only are volatile phenols carried by smoke, but additionally glycoconjugate forms of these phenols are present as will. These have been found to have a large impact on the flavor of wines, being the cause of a lasting ashy aftertaste post consumption (Parker et al, 2012). When evaluating the sensory profile of these wines when tasted one after the other, there is an observed problem due to the lasting nature of these undesirable attributes and high level of carry-over from sample to sample. The aim of this work is to evaluate the extent this carryover occurs, along with the best sensory practices to mitigate its influence via different inter-stimulus rinse protocols.

In table grapes seedlessness is a crucial breeding target, mainly results from stenospermocarpy, linked to the Thompson Seedless variety. Several studies investigated the genetic control of seedlessness identifying AGL11, a MADS-box transcription factor, as a crucial gene.
We performed a deep investigation of the whole AGL11 gene sequence in a collection of grapevine varieties revealing three different promoter-CDS combinations. By investigating the expression of the three AGL11 alleles and evaluating their ability to activate the promoter region, we show that AGL11 regulates its transcription in a specific promoter-CDS manner. By a multi-AGL11 co-expression analysis we identified a methyl jasmonate esterase, an indole-3-acetate beta-glucosyltransferase, and an isoflavone reductase as top AGL11 candidate targets. In vivo experiments further confirmed AGL11 role in regulating these genes, demonstrating its significant influence in seed development and thus in seedlessness trait.

The Registered Appellation of Origin Mark (RAOM) « Jerez-Xérès-Sherry and Manzanilla Sanlucar de Barrameda » is one of the oldest and more important zone in wine history and production. «Albarizas» unit (white calcareous marls with sea-fossils) is the most representative geological material of the RAOM (75%) and even more in the central-NW area of the RAOM, known as «Jerez Superior» area (Superior Quality Sherry Area). « Albarizas » form undulated hillocks (3-10% slope) and hills (>10% slope), the litologic unit has E-W and S-W direction, and Regosols and Leptosols are the principal soils.