terclim by ICS banner
IVES 9 IVES Conference Series 9 Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Volatile Organic Compound markers of Botrytis cinerea infection in artificially inoculated intact grape berries

Abstract

The addition of partially dehydrated grapes to enrich must composition for producing complex dry/sweet wines represents a traditional practice in several regions of the world. However, the environmental conditions of dehydration chambers may facilitate the infection of Botrytis cinerea Pers. by promoting disease and provoking large grape losses. B. cinerea attack can induce alterations in the profile of volatile organic compounds (VOCs), which could be detected by sensors specifically trained to detect infection/disease-related compounds. These sensors could facilitate the early detection of the infection, consequently allowing to adjust some dehydration parameters. To deepen the understanding on alterations induced by B. cinerea on intact grape VOCs profile, berries from Sangiovese and Corvina cultivars were collected and analysed by SPME-GC-MS as such and following artificial inoculation with a spore suspension of B. cinerea (10 μl, 105 spores ml-1) or mock inoculation by using the same volume of sterile growth medium (control). Preliminary results have shown that high levels of a set of primary and secondary alcohols appeared to be emitted by inoculated berries. Some of these molecules are already reported as correlated with B. cinerea infection, while others are not mentioned as infection markers yet. Moreover, the dynamics of the emission of some of these compounds during the in vitro development of B. cinerea cultures have also been studied. Setting up sensors capable of detecting the identified volatile markers in the dehydration chambers represents an ambitious goal for reducing spoilage and grape losses via targeted interventions.

DOI:

Publication date: June 14, 2024

Issue: Open GPB 2024

Type: Poster

Authors

Pietro Emilio Nepi1*, Claudia Pisuttu2, Cristina Nali2, Elige Salame1, Pietro Tonutti1, Stefano Brizzolara1

1Crop Science Research Center, Scuola Superiore Sant’Anna di Studi Universitari, Piazza Martiri della Libertà,33,56127, PISA, ITALY
2Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80,56124, PISA, ITALY.(Left-aligned, italic, Arial 9)

Contact the author*

Keywords

grape, grey mould, VOCs, sensors, sustainability

Tags

IVES Conference Series | Open GPB | Open GPB 2024

Citation

Related articles…

Budburst delay and berry ripening after vegetal oil application in Austria

Occurrence of freezing temperatures in early spring when grapevine shoots are developing is termed late frost in viticulture. Young green tissues are very sensible to temperatures below zero and damages often lead to important yield and quality losses such as the case in Europe in 2017. An indirect method to avoid late frost damage in vineyards consist in delaying the budburst. Previous research reported similar effects by applying vegetal oil on dormant buds. Here, we tested the application of rapeseed vegetal oil during late winter to delay the budburst on two V.vinifera cultivars of interest in Austria, Grüner Veltliner (GV) and Zweigelt (ZW).

EFFECTS OF DIFFERENT PRUNING TYPES ON CHARENTE UGNI BLANC GRAPE AND WINE QUALITY

Since the use of sodium arsenite was banned in 2001, Grapevine Trunk Diseases (GTDs) have become even more widespread increasing (1).To avoid pathogen entry, pruning, an age-old practice, is increa- singly coming to the fore. As the vine is a liana (2), any excessive woody proliferation has to be stopped. This can preserve grapevine life, provided it does not damage the diaphragm.

The impact of global warming on Ontario’s icewine industry

Ontario’s wine regions lie at the climatic margins of commercial viticulture owing to their cold winters and short cool growing season. The gradual warming of northern latitudes projected under a human-induced climate change scenario could bring mixed benefits to these wine regions.

Effect of rootstock and preplant fumigation on plant parasitic nematode development in Washington wine grapes

In Washington State, the majority of winegrape (Vitis vinifera) vineyards are planted to their own roots. This practice is possible due to the lack of established phylloxera populations, and is preferred due to the ease of retraining after damaging winter cold events. However, own-rooted V. vinifera is generally susceptible to most plant parasitic nematodes that attack grape. In Washington State, management of nematodes is dominated by preplant soil fumigation. One practice that may mitigate economic loss due to nematodes is the adoption of nematode-“resistant” rootstocks.

Characterization of resistant varieties produced in the context of a search for regional typicality

Planted between 2018 and 2019, the ‘New Vine’ system is a vineplot, comprising 169 individuals genotypes (5 vines/individual), located on a gravelous soil, in the INRAE Grande-Ferrade site (Villenave d’Ornon, France).