Macrowine 2021
IVES 9 IVES Conference Series 9 Effect of plant fining agents in the must flotation process. Functional characterization

Effect of plant fining agents in the must flotation process. Functional characterization

Abstract

Flotation is one of the most used processes for clarifying white grape must after the pressing process. To date, gelatine is the more used fining agent, its action being improved when combined with bentonite and silica sol. However, in recent years, there is a growing commercial interest in replacing this animal origin protein with plant proteins, due, on the one hand, to the problems associated with allergies and, on the other hand, also thinking in the vegan wine consumers. However the efficiency of plant proteins as floculating agents are lower than gelatine and varies among them, the reason behind the different behaviour being unknown (Marchal et al., 2003; Gambuti et al., 2016; Petinelli et al., 2020). The objective of this work was to compare the flocculating efficiency of a commercial gelatine, a pure pea protein and the same pea protein chemically modified and to relate this efficiency to their amino acid composition and protein functions.The determination of the efficiency was carried out by measuring the percentage of clean must after 1 h from flotation beginning and the measuring of the absorbance at 440 nm to control the browning, while the content of amino acids and peptides was carried out by HPLC-MS. The UNIPROT database was used to obtain the protein functions associated with the peptidesThe results showed that the modified pea protein showed very similar effciency as flotation agent to that of the commercial gelatine and higher than the pure pea protein. The important structural modification made to the pea protein made it more reactive, probably due to a higher exposure of its structure and the apolar and positively charged amino acids, and to the appearance of peptides with protein functions of binding to carbohydrates and proteins, which are also present in animal protein. With these results it can be concluded that pure vegetal proteins may not have sufficientt functional properties to behave as good flocculating agents, although certain chemical modification in their structure may further simulate the behaviour shown by animal protein.

DOI:

Publication date: September 7, 2021

Issue: Macrowine 2021

Type: Article

Authors

Ana Belén Bautista-Ortín, Sonia, Albendea-Roa,  Jurado

University of Murcia,Bermúdez-Galvez, (University of Murcia) Gómez-Plaza, Encarna (University of Murcia), Mar (Agrovin S.A.), Ricardo (Agrovin, S.A.)

Contact the author

Keywords

finning, amioacids, proteins, flotation, white wine

Citation

Related articles…

Isolation, biofilm formation and control of the wine spoilage yeast Brettanomyces bruxellensis

Brettanomyces bruxellensis, commonly referred to as “Brett,” is one of the most notorious microorganisms implicated in wine spoilage. This yeast species has developed a noteworthy resistance to sulfur dioxide, a widely used preservative in winemaking, prompting the wine industry to seek new antimicrobial agents.

New breeding frontiers: application of the CRISPR-cas9 system in grapevine (V. vinifera L.) and improvements in plant regeneration

Nowadays, many policies are being adopted for direct agriculture towards more sustainable approaches. To continue to maintain a high production using fewer fertilizers, pesticides and water resources, agronomic techniques must be combined with biotechnological approaches. In grapevine, the breeding programs are restricted by the fact that it has a highly heterozygous genome, therefore, if on the one hand, we try to improve the characteristics, on the other hand it is necessary to preserve the original genome of the varieties. CRISPR-cas9 system is one of the smartest tools to carry out highly precise genetic modifications leaving the genetic background unchanged.

Permanent vs temporary cover crops in a Sangiovese vineyard: preliminary results on vine physiology and productive traits

Cover crops in vineyards have been extensively studied, as the choice of grass species and their management significantly influence soil properties and vine performance.

MOVING FROM SULFITES TO BIOPROTECTION: WHICH IMPACT ON CHARDONNAY WINE?

Over the last few years, several tools have been developed to reduce the quantity of sulfites used during winemaking, including bioprotection. Although its effectiveness in preventing the development of spoilage microorganisms has been proven, few data are available on the impact of sulfite substitution by bioprotection on the final product. The objective of this study was therefore to characterize Chardonnay wines with the addition of sulfite or bioprotection in the pre-fermentation stage. The effects of both treatments on resulting matrices was evaluated at several scales: analysis of classical oenological parameters, antioxidant capacity, phenolic compounds, non-volatile metabolome and sensory profile.

Evaluation of the effects of pruning methodology on the development of young vines 

Grapevine pruning is one of the most important practices in the vineyards. Winegrowers use it to provide the vines the shape needed, or to maintain it once achieved, and also to balance vegetative growth and fruit production. In the last decades, careless pruning has been blamed, among other factors, as responsible of the vineyard decay that is been observed even in young vines. However, to our knowledge, there is a lack of systematic research trying to elucidate to which extent the pruning method used affects plant development or its susceptibility to grapevine trunk diseases (GTD). Within this context, the aim of this work is to study the influence of different pruning method strategies on the development of field-planted young vines.