The goal of this work was to determine the spatial variability of terroir using the isotope composition of wine. Carbon (δ13C) and oxygen (δ18O) stable isotope composition was measured in wines from Tempranillo (Vitis vinifera L.) vineyard, located in Rioja Appellation (Spain). Stable isotope composition, leaf area, vigour, yield components, grape and wine composition were determined in a grid of 85 geo-referenced points, that was drawn across the 5 ha vineyard area