Grapevine nitrogen (N) monitoring is essential for efficient N management plans that optimize fruit yield and quality while reducing fertilizer costs and the risk of environmental contamination. Unlike traditional vegetative-tissue sampling methods, remote sensing technologies, including hyperspectral imaging, have the potential to allow monitoring of the N status of entire vineyards at a per-vine resolution. However, differential N partitioning, variable spectral properties, and complex canopy structures hinder the development of a robust N retrieval algorithm. The present study aimed to establish a solid understanding of vine spectroscopic response at leaf and canopy levels by evaluating the different nitrogen retrieval approaches, including the radiative transfer model.