Eléments importants d’une méthodologie de caractérisation des facteurs naturels du terroir, en relation avec la réponse de la vigne à travers le vin

“The Human being has an excellent ability to detect and discriminate odors but typically has great difficulty in identifying specific odorants”(1). Furthermore, “from a cognitive point of view the mechanism used to judge wines is closer to pattern recognition than descriptive analysis.” Therefore, when one wants to reveal the volatile “wine-like feature” pattern recognition techniques are required. Sensomics is one of the most recent “omics”, i.e. a holistic perspective of a complex system, which deals with the description of substances originated from microorganism metabolism that are “active” to human senses (2). Depicting the relevant volatile fraction in wines has been an ongoing task in recent decades to which several research groups have allocated important resources. The most common strategy has been the “target approach” in order to identify the “key odorants” for a given wine varietal.

The study of the interaction between vineyards and the environment to establish the grapevines in the appropriate places has been applied in wine science for 5000 years. Advances in the field of the zoning have not been uniform in time, and have occupied a preferential place in the contributions of Roman writers of the 1st Century AC, the contemplations of Tokay (1700) and Porto (1756) and works of the second half of the 20th century. Zoning practices today integrate multidisciplinary methodologies (viticulture, enology, soils, climatology, cartography, statistics, computer science) and require further development for future application.

Cis-acting regulatory elements are DNA sequences that can be bound by transcription factors to regulate the expression of genes in a condition-dependent and tissue-specific way. It is nowadays possible to search for DNA motives and sequences that a given transcription factor is binding or at least can, but it is still hard to have a glance at all the transcription factors that are contemporaneously located at the same locus. Inspired by an existing technique that uses the CRISPR-Cas system in mammal cells, we are trying to develop a protocol to study such regulation in Vitis vinifera. Using the highly sequence-specific binding capacity of a catalytically inactive Cas9 protein (dCas9), our idea is to set up a system to target a desired sequence and precipitate all the crosslinked proteins and distantly interacting chromatin at this locus and analyze them.

The aging potential of Burgundy chardonnay wines is considered as quality indicator. However, some of them exhibit higher oxidative sensitivity and premature oxidative aging symptoms, which are potentially induced by no-enzymatic oxidation such as Fenton-type reaction (Danilewicz, 2003). This chemical mechanism involves the action of transition metal, native phenolic compounds and oxygen which promote the generation of highly reactive oxygen species (ROS) such as hydroxyl radicals (OH) or 1-hydroxyethyl radicals (1-HER) from oxidation of ethanol. Such mechanism is involved in the radical oxidation occurring during bottle aging. According to Elias et al.,(2009a), the 1-HER is the most abundant radical in forced oxidation treated wines. Consequently, understanding its evolution kinetic in dry white wines is of great importance.

Grapevine is one of the crops that may suffer more negative impacts
under climate change, due not only to changes in temperature but also due to water available. Some of the most direct effects of climate variability on grapevines are the changes in the onset and timing of phenology events and changes in the length of the growing season, which may have further effects on grape quality. The aim of this research was to analyze the changes in vine phenology of some red varieties (Tempranillo, Grenache and Carignan) cultivated in Rioja Oriental (Rioja DOCa), under different climate change scenarios.