Quelles cibles moléculaires pourraient expliquer l’effet du terroir sur la composition des baies en sucres et acides?

Situated at the beginning of the 20th century on the territory of the Chablis municipality, delimited according to specialists of the time to plots of “kimmeridgian” origin, the vineyard producing Chablis

Astringency is one of the leading quality factors in red wines.

The southern Côtes-du-Rhône vineyard shows a significant variety of ecological facets over the Lower Rhone Valley. Intending to characterize such a variety of “terroir “called vineyard situations, a spatial approach based on identification of soil landscapes has been initiated.

The evolution of cultivated plants played important role in the ascent of humanity. Research of their origin and evolution started at the beginning of the20th century, but till nowadays a lot of questions remain open. A large number of theories exist about the evolution of the European grapevine (Vitis vinifera L.). The Vitis sylvestris GMEL. in Hungary is a protected species.

Developments in high-throughput sequencing (HTS) technologies allow us to obtain large amounts of microbial information from wine and must samples. Thus approaches, that are aimed at characterising the microbial diversity during fermentation, can be enhanced, or possibly even replaced, with HTS-based metabarcoding. To reduce experimental biases and increase data reproducibility, we compared 3 DNA extraction methods by evaluating differences in the fungal diversity with Riesling alcoholic fermentation samples at four different vineyards. The fungal diversity profiling was done using the genetic markers ITS2 and D2 using metabarcoding. The extraction methods compared consisted of a commercial kit, a recently published protocol that includes a DNA enhancer, and a protocol based on a buffer containing common inhibitor removal reagents. All methods were able to distinguish vineyard effects on the fungal diversity, but the results differed quantitatively.